A kind of high-efficiency fermentation method of aflatoxin degrading bacteria

An aflatoxin and fermentation method technology is applied in the field of high-efficiency fermentation of aflatoxin-degrading bacteria, which can solve the problems of increasing production cost, increasing energy consumption, etc. Effect

Active Publication Date: 2021-08-06
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Traditional ways to increase ventilation include increasing stirring rate, increasing ventilation, etc., but these methods will undoubtedly increase energy consumption and increase production costs in actual production applications

Method used

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  • A kind of high-efficiency fermentation method of aflatoxin degrading bacteria
  • A kind of high-efficiency fermentation method of aflatoxin degrading bacteria
  • A kind of high-efficiency fermentation method of aflatoxin degrading bacteria

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] (1) Strain cultivation: Before the experiment, the strains were taken out from the refrigerator, kept warm with crushed ice, and an appropriate amount of strains were picked up with sterilized bamboo sticks or pipette tips and inoculated on LB agar medium, and cultivated in a 37°C incubator for 12- 24 hours to activate the strains, and when colonies are formed, pick a single colony with a sterilized bamboo stick and inoculate it on a new LB agar medium, and cultivate it in a 37°C incubator for 12-24 hours before use;

[0028] (2) Preparation of seed solution: pick a single colony from the secondary transformation medium with a sterilized bamboo stick and inoculate it into LB medium, and cultivate it at 37°C and a shaker speed of 180rpm for 12h to obtain a seed solution;

[0029] (3) Gradient experiment of the oxygen supply condition of the strain: the seed solution was inoculated into the LB medium (250mL Erlenmeyer flask, liquid volume 50mL) at a ratio of 2% by volume f...

Embodiment 2

[0033] (1) Strain cultivation: Before the experiment, the strains were taken out from the refrigerator, kept warm with crushed ice, and an appropriate amount of strains were picked up with sterilized bamboo sticks or pipette tips and inoculated on LB agar medium, and cultivated in a 37°C incubator for 12- 24 hours to activate the strains, and when colonies are formed, pick a single colony with a sterilized bamboo stick and inoculate it on a new LB agar medium, and cultivate it in a 37°C incubator for 12-24 hours before use;

[0034] (2) Preparation of seed solution: pick a single colony from the secondary transformation medium with a sterilized bamboo stick and inoculate it into LB medium, and cultivate it at 37°C and a shaker speed of 180rpm for 12h to obtain a seed solution;

[0035] (3) Selection of oxygen-carrying agent types and comparison of addition amount: Three hydrocarbons were selected as the screening objects of oxygen-carrying agents: n-hexadecane, dodecane, and n-...

Embodiment 3

[0040] (1) Strain cultivation: Before the experiment, the strains were taken out from the refrigerator, kept warm with crushed ice, and an appropriate amount of strains were picked up with sterilized bamboo sticks or pipette tips and inoculated on LB agar medium, and cultivated in a 37°C incubator for 12- 24 hours to activate the strains, and when colonies are formed, pick a single colony with a sterilized bamboo stick and inoculate it on a new LB agar medium, and cultivate it in a 37°C incubator for 12-24 hours before use;

[0041] (2) Preparation of seed solution: pick a single colony from the secondary transformation medium with a sterilized bamboo stick and inoculate it into LB medium, and cultivate it at 37°C and a shaker speed of 180rpm for 12h to obtain a seed solution;

[0042] (3) The effect of oxygen-carrying agent addition time on fermentation: add 1% of the three oxygen-carrying agents at the time of inoculation and after 4 hours of cultivation, culture for about 24...

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Abstract

The invention discloses a high-efficiency fermentation method of aflatoxin-degrading bacteria, which belongs to the field of biotechnology and includes the steps of seed liquid preparation, fermentation culture, adding oxygen-carrying agent, and measuring the content of aflatoxin; The method of adding dodecane as an oxygen-carrying agent in the fermentation system of toxin-degrading bacteria can improve the fermentation efficiency of the target strain, and the obtained fermentation product of the target strain can significantly reduce the residual rate of aflatoxin. The supernatant fermented for 20 hours Compared with the blank control group without oxygen carrier, the residual rate of aflatoxin was reduced by 24.41%, and the residual rate of aflatoxin was only 9.6%.

Description

technical field [0001] The invention belongs to (relates to) the technical field of microorganisms, and in particular relates to a high-efficiency fermentation method of aflatoxin-degrading bacteria added with an oxygen-carrying agent. Background technique [0002] Aflatoxins are a class of secondary metabolites containing a dihydrofuran ring structure, and are a class of highly toxic natural toxins. It is mainly biosynthesized by Aspergillus flavus and Aspergillus parasiticus under certain environmental conditions. Aflatoxins are highly toxic and highly carcinogenic to humans, and are widely distributed in moldy grains and nuts (including rice, corn, peanuts, cottonseed, etc.), contaminated milk and dairy products. There are 18 kinds of aflatoxins that have been discovered so far. The most common aflatoxins are mainly aflatoxins B1, B2, G1 and G2. carcinogens. The International Agency for Research on Cancer (IARC) lists aflatoxins as a class I carcinogen. Aflatoxins hav...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A23L5/20C12R1/07
CPCA23L5/28C12N1/20C12N1/205C12R2001/07
Inventor 周文文韦显雪王树雯钱超
Owner ZHEJIANG UNIV
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