Compound microorganism controlling cyanobacterial bloom as well as preparation method and application of compound microorganism

Abstract

The invention discloses a compound microorganism controlling cyanobacterial bloom. The compound microorganism comprises components in parts by weight as follows: 10-30 parts of bacillus laterosporus and 10-30 parts of bacillus amyloliquefaciens. The compound microorganism can effectively degrade organic pollutants in water, removes nitrogen and phosphorus, controls and reduces the cyanobacterial bloom, and is free of side effects on the environment and humans. The invention further discloses a preparation method and an application of the compound microorganism.

Description

technical field

[0001] The invention relates to the technical field of water treatment. More specifically, it relates to a compound microorganism for controlling cyanobacteria bloom, its preparation method and application. Background technique

[0002] With the rapid development of social economy and the rapid increase of population, a large amount of untreated industrial wastewater, domestic sewage and agricultural wastewater are discharged into natural waters indiscriminately, causing the limited fresh water resources of human beings to be seriously polluted by nitrogen, phosphorus and organic matter, and the water body is rich. The process of trophicization is accelerated, which in turn causes algal blooms to occur frequently, which has caused a food chain in terms of human health, ecology and landscape, affecting the health of humans and aquatic animals, especially certain strains of cyanobacteria or the secondary metabolism produced The product microcystin can damage t...

Examples

preparation example Construction

[0044] According to yet another embodiment of the present invention, the preparation method of above-mentioned compound microorganism is provided, and this method comprises the steps:

[0045] Carrying out single-strain fermentation of Bacillus lateralis and Bacillus amyloliquefaciens;

[0046] The composite microorganisms are obtained by mixing raw materials including fermented Bacillus disporosporum and Bacillus amyloliquefaciens.

[0047] The above-mentioned Bacillus lateralis and Bacillus amyloliquefaciens are preferably extracted from water bodies where cyanobacteria blooms occur. The extraction method preferably comprises the steps of:

[0048] Take the water body where the cyanobacteria bloom broke out, shake it to disperse the microbial cells in the water body evenly, and dilute the water body to 10 -1 、10 -2 、10 -3 、10 -4 、10 -5 、10 -6 、10 -7 、10 -8 diluent;

[0049] the 10 -6 、10 -7 、10 -8 Mixed with the diluted solution, mixed evenly with the culture me...

Embodiment 1

[0056] A compound microorganism for controlling cyanobacteria bloom, its weight percentage includes the following components: TC bacteria A 20%, TC bacteria B 20%, complex enzyme 10%, trace elements 30%, carrier 20%;

[0057] Wherein, the above-mentioned TC bacteria A is Bacillus lateralis;

[0058] TC bacteria B is Bacillus amyloliquefaciens;

[0059] The compound enzyme includes: 30% pectinase, 30% dextranase, 40% cellulase according to the mass percentage, and the three are mixed;

[0060] Trace elements include: potassium dihydrogen phosphate 15%, magnesium sulfate 15%, sodium acetate 15%, ferrous sulfate 15%, sodium silicate 40%, mixed according to mass percentage;

[0061] The carrier is amino polysaccharide, which is a biosynthetic natural polymer with a molecular weight between 1,000,000-2,000,000 and a degree of deacetylation as high as 80%. 1→4) The glycoside chains are connected with each other.

[0062] The preparation method of above-mentioned composite microor...

Embodiment 2

[0086] Repeat embodiment 1, difference is, the composite microorganism of controlling cyanobacteria bloom, its percentage by weight comprises the following components:

[0087] TC bacteria A30%, TC bacteria B 10%, compound enzyme 20%, trace elements 20%, carrier 20%;

[0088] Wherein, the above-mentioned TC bacteria A is Bacillus lateralis;

[0089] TC bacteria B is Bacillus amyloliquefaciens;

[0090]The compound enzyme includes: 25% pectinase, 30% glucanase, 45% cellulase according to the mass percentage, and the three are mixed;

[0091] Trace elements include: 14% potassium dihydrogen phosphate, 13% magnesium sulfate, 14% sodium acetate, 16% ferrous sulfate, 43% sodium silicate,

[0092] The rest of the conditions remained unchanged, and the composite microorganism was prepared.

[0093] The above-mentioned composite microorganisms are added into the water body where the cyanobacteria bloom explodes in an amount of 4 ppm.

technical field

[0001] The invention relates to the technical field of water treatment. More specifically, it relates to a compound microorganism for controlling cyanobacteria bloom, its preparation method and application. Background technique

[0002] With the rapid development of social economy and the rapid increase of population, a large amount of untreated industrial wastewater, domestic sewage and agricultural wastewater are discharged into natural waters indiscriminately, causing the limited fresh water resources of human beings to be seriously polluted by nitrogen, phosphorus and organic matter, and the water body is rich. The process of trophicization is accelerated, which in turn causes algal blooms to occur frequently, which has caused a food chain in terms of human health, ecology and landscape, affecting the health of humans and aquatic animals, especially certain strains of cyanobacteria or the secondary metabolism produced The product microcystin can damage t...

Examples

preparation example Construction

[0044] According to yet another embodiment of the present invention, the preparation method of above-mentioned compound microorganism is provided, and this method comprises the steps:

[0045] Carrying out single-strain fermentation of Bacillus lateralis and Bacillus amyloliquefaciens;

[0046] The composite microorganisms are obtained by mixing raw materials including fermented Bacillus disporosporum and Bacillus amyloliquefaciens.

[0047] The above-mentioned Bacillus lateralis and Bacillus amyloliquefaciens are preferably extracted from water bodies where cyanobacteria blooms occur. The extraction method preferably comprises the steps of:

[0048] Take the water body where the cyanobacteria bloom broke out, shake it to disperse the microbial cells in the water body evenly, and dilute the water body to 10 -1 、10 -2 、10 -3 、10 -4 、10 -5 、10 -6 、10 -7 、10 -8 diluent;

[0049] the 10 -6 、10 -7 、10 -8 Mixed with the diluted solution, mixed evenly with the culture me...

Embodiment 1

[0056] A compound microorganism for controlling cyanobacteria bloom, its weight percentage includes the following components: TC bacteria A 20%, TC bacteria B 20%, complex enzyme 10%, trace elements 30%, carrier 20%;

[0057] Wherein, the above-mentioned TC bacteria A is Bacillus lateralis;

[0058] TC bacteria B is Bacillus amyloliquefaciens;

[0059] The compound enzyme includes: 30% pectinase, 30% dextranase, 40% cellulase according to the mass percentage, and the three are mixed;

[0060] Trace elements include: potassium dihydrogen phosphate 15%, magnesium sulfate 15%, sodium acetate 15%, ferrous sulfate 15%, sodium silicate 40%, mixed according to mass percentage;

[0061] The carrier is amino polysaccharide, which is a biosynthetic natural polymer with a molecular weight between 1,000,000-2,000,000 and a degree of deacetylation as high as 80%. 1→4) The glycoside chains are connected with each other.

[0062] The preparation method of above-mentioned composite microor...

Embodiment 2

[0086] Repeat embodiment 1, difference is, the composite microorganism of controlling cyanobacteria bloom, its percentage by weight comprises the following components:

[0087] TC bacteria A30%, TC bacteria B 10%, compound enzyme 20%, trace elements 20%, carrier 20%;

[0088] Wherein, the above-mentioned TC bacteria A is Bacillus lateralis;

[0089] TC bacteria B is Bacillus amyloliquefaciens;

[0090]The compound enzyme includes: 25% pectinase, 30% glucanase, 45% cellulase according to the mass percentage, and the three are mixed;

[0091] Trace elements include: 14% potassium dihydrogen phosphate, 13% magnesium sulfate, 14% sodium acetate, 16% ferrous sulfate, 43% sodium silicate,

[0092] The rest of the conditions remained unchanged, and the composite microorganism was prepared.

[0093] The above-mentioned composite microorganisms are added into the water body where the cyanobacteria bloom explodes in an amount of 4 ppm.