Polyculture type microalgae cultivation method capable of inhibiting growth of microcystis aeruginosa

A technology for culturing Microcystis aeruginosa and microalgae, which is applied in the directions of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of influence, secondary pollution, and high cost, and achieves strong operability and growth inhibition. , the effect of promoting growth

Inactive Publication Date: 2016-08-24
EAST CHINA UNIV OF SCI & TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

If chemical agents and other methods are used to inhibit the growth of toxic microalgae in the water body, while killing the toxic microalgae, it will also affect other beneficial microorganisms in the water body. In addition, the cost is high and it will cause secondary pollution.

Method used

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  • Polyculture type microalgae cultivation method capable of inhibiting growth of microcystis aeruginosa
  • Polyculture type microalgae cultivation method capable of inhibiting growth of microcystis aeruginosa

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Embodiment 1-3

[0039] Three kinds of non-toxic microalgae (Chlorella pyrenoidosa FACHB-1222, Chlorella ellipsoides FACHB-40 and Scenedesmus obliquus FACHB-417) were mixed with Microcystis aeruginosa.

[0040] Chlorella pyrenoidosa FACHB-1222, Chlorella ellipsoides FACHB-40, Scenedesmus obliquus FACHB-417, and Microcystis aeruginosa were cultured in BG11 medium, and expanded.

[0041] Take three kinds of non-toxic microalgae and Microcystis aeruginosa in good growth condition respectively into 100mL centrifuge tubes, and put them in a refrigerated centrifuge at 5000r min -1 Centrifuge for 5 minutes; discard the supernatant, wash the centrifuged algae repeatedly with sterile deionized water for 3 times, and dilute with sterile deionized water to be inoculated.

[0042] Three kinds of non-toxic microalgae and Microcystis aeruginosa were inoculated in TN=15mg L according to the ratio shown in Table 1 -1 ,TP=0.3mg·L -1 The secondary effluent water, the cultivation condition is under the natural...

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Abstract

The invention relates to a polyculture type microalgae cultivation method capable of inhibiting growth of microcystis aeruginosa. Secondary effluent water of an urban sewage plant serves as a culture medium, the nontoxic microalgae and toxic cyanobacteria are subjected to joint culture in the secondary effluent water of the urban sewage plant, the density proportion between the initial nontoxic microalgae and toxic cyanobacteria is controlled to be (2:1)-(10:1), in the culture process, sodium nitrate and monopotassium phosphate serve as complementation sources of nitrogen and phosphorus respectively, the concentration of the culture medium is controlled to meet the conditions that total nitrogen (TN)=14.0-16.0 mg.L<-1>, total phosphorus (TP)=0.28-0.40 mg.L<-1>, and the proportion between nitrogen and phosphorus is (40-50): 1. Compared with the prior art, the polyculture type microalgae cultivation method is applicable to nontoxic microalgae culture, inhibition to growth of the microcystis aeruginosa is achieved, and the method is a safe, reliable and economic cultivation method; besides, according to the technical method, the secondary effluent water of the urban sewage plant serves as a culture medium source, and the function of deeply purifying sewage is achieved.

Description

technical field [0001] The invention relates to a method for cultivating microalgae, in particular to a method for cultivating polyculture microalgae capable of inhibiting the growth of Microcystis aeruginosa. Background technique [0002] At present, in urban sewage treatment, the concentration of nitrogen and phosphorus in the effluent often exceeds the standard. If it is directly discharged into the natural water body, it will lead to eutrophication of the water body, cause the vicious reproduction of toxic microalgae, and cause algal blooms; the secondary effluent has a large amount of water and a low content of organic matter. If the deep denitrification and dephosphorization treatment technology is adopted, investment and operation costs will be increased. [0003] Non-toxic microalgae are a kind of microorganisms that are common in natural water bodies and have the value of ecological resource utilization. The use of non-toxic microalgae as biomass energy raw material...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C12N1/20C12R1/89
CPCC12N1/12C12N1/20
Inventor 陈秀荣王璐徐燕王晓晓张玉莹李佳慧于泽亚
Owner EAST CHINA UNIV OF SCI & TECH
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