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Application of ginkgolic acid in killing of blue-green algae

A technology of ginkgolic acid and blue-green algae, applied in the fields of application, biocide, and chemicals for biological control, etc., can solve the problems of less research on terrestrial plants, and achieve drug resistance, environmental friendliness, and simple use methods Effect

Inactive Publication Date: 2013-09-18
北京鑫洋水产高新技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of these studies focus on the interaction between aquatic plants and cyanobacteria, and there are few studies on terrestrial plants.

Method used

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  • Application of ginkgolic acid in killing of blue-green algae
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  • Application of ginkgolic acid in killing of blue-green algae

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0070] 1. Experimental steps

[0071] (1) Extraction and preliminary separation of test samples

[0072] Under normal temperature conditions, 20kg of Ginkgo biloba exocarp crushed material was extracted with absolute industrial ethanol (200L) at 75°C under reflux for 3 times, each time for 2h, the extracts were combined and concentrated under reduced pressure to obtain 6640g of ethanol extract. Then the obtained ethanol extract is extracted 3-5 times with an equal volume of sherwood oil, until the extract is almost colorless, the extract is concentrated under reduced pressure to obtain 3276g of sherwood oil extract, the extraction rate is 49.3% by weight, and the remaining residue is 3300g .

[0073] (2) Separation of petroleum ether extract

[0074] (a) Sample handling

[0075] Take 100g of petroleum ether extract extract, dissolve it in methanol, add 100g of silica gel, mix well, put it in a constant temperature water bath (temperature 50°C), evaporate to dryness, and gri...

Embodiment 2

[0103] B-2 grouped samples (wherein, the content of ginkgolic acid is 96.70% by weight) were tested for algicidal activity according to the above-mentioned method.

[0104] The algicidal experiment results (expressed as algae inhibition rate) were counted on the 3rd day and the 7th day respectively. Table 1 shows the results of algae inhibition experiments of different concentrations of B-2 stream grouping samples.

[0105] Table 1

[0106]

[0107] Calculate the LC on the 3rd and 7th day according to the algal inhibition rate on the 3rd and 7th day 50 They are 4.25mg / L and 3.41mg / L respectively.

[0108] The above results indicate that the B-2 subgroup sample, ie, ginkgolic acid has excellent killing activity against Microcystis aeruginosa.

Embodiment 3

[0110] Take 10.0 kg of dried Ginkgo biloba testa and grind it to 30-60 mesh. Use industrial methanol to reflux extraction, pressurize and concentrate to obtain a fluid extract, fully volatilize the methanol, stir and dissolve with industrial ethanol, take the supernatant, and remove sediment sugar, starch and other impurities. After concentrating the supernatant, dissolve and extract with petroleum ether, remove the residue, concentrate the extract to extract A, and use the HPLC method in Example 1 to measure the content of ginkgolic acid in this extract A to be 40% by weight.

[0111] Mix 62.5 volumes of extract A and 25 volumes of methanol evenly, add 12.5 volumes of emulsifier, and stir vigorously to obtain algicide A. When in use, first dilute the algicide A with 1000 times of water, and then sprinkle the whole pool, the volume of the algicide A per cubic meter is 1-3mL.

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Abstract

The invention discloses application of ginkgolic acid in killing of a blue-green algae, application of an alcohol extract of ginkgo biloba sarcotesta in killing of the blue-green algae, a composition and application of the composition in killing of the blue-green algae. It is observed that the ginkgolic acid and a preparation containing the ginkgolic acid have strong activity of killing the blue-green algae from the embodiment of the invention. In addition, the ginkgolic acid is plant source algicide, is taken from the natural world and applied to the natural world, is low in toxicity and residue, and is an algicide which is friendly to the environment, and simple in using method; and the drug resistance is not easily generated.

Description

technical field [0001] The invention relates to the application of ginkgolic acid in killing cyanobacteria. Background technique [0002] In the past ten years, the frequency of water blooms (cyanobacteria) in some waters of my country has become higher and higher, and the economic losses brought about have become larger and larger (Zhao Yuheng, Yang Hongsheng. Research on a kind of euglena bloom in fish ponds. Journal of Hydrobiology, 1994, 18(2): 186-188), algal blooms have become a problem that seriously affects the quality of the water environment and the ecological safety of water bodies, not only greatly endangering human health and other biological safety, but also causing huge economic losses. According to literature reports, the species that form water blooms in these waters mainly include Cyanophyta Microcystis and Astringentia, among which Microcystis aeruginosa dominates (Heath R.L, Parker L. Photoperitation in isolated choloplasts kinetics and stoichiometry of f...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N37/40A01P1/00A01N65/00
Inventor 夏磊赵明军张洪玉范毛毛杨仲明彭翔王高学张超
Owner 北京鑫洋水产高新技术有限公司
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