Complex microbial inoculant for preventing and treating cyanobacterial bloom in aquaculture as well as preparation method and application of complex microbial inoculant

A compound bacterial agent, cyanobacterial bloom technology, applied in the directions of microorganism-based methods, biochemical equipment and methods, chemical instruments and methods, etc., to achieve the effect of improving water quality aging and water turbidity regulation

Active Publication Date: 2019-04-09
天津开发区坤禾生物技术有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

After retrieval, there is no report on the prevention and treatment of cyanobacteria blooms with only compound bacteria agents (Bacillus subtilis and Bacillus amyloliquefaciens)

Method used

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  • Complex microbial inoculant for preventing and treating cyanobacterial bloom in aquaculture as well as preparation method and application of complex microbial inoculant
  • Complex microbial inoculant for preventing and treating cyanobacterial bloom in aquaculture as well as preparation method and application of complex microbial inoculant
  • Complex microbial inoculant for preventing and treating cyanobacterial bloom in aquaculture as well as preparation method and application of complex microbial inoculant

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Embodiment 1

[0044] The invention provides a composite bacterial agent for preventing and controlling cyanobacteria blooms in aquaculture. The composite bacterial agent includes fermented bacterial powder of Bacillus subtilis MES810 and fermented bacterial powder of Bacillus amyloliquefaciens MES 812. The mass ratio of the latter is 1:1. Among them, the number of live bacteria in the fermentation powder of Bacillus subtilis MES810 is not less than 2.0×10 10 cfu / g, the number of live bacteria in the fermentation product of Bacillus amyloliquefaciens MES 812 is not less than 4.0×10 9 cfu / g.

[0045] Among the composite bacterial agents for preventing and controlling cyanobacteria blooms in aquaculture of the present invention, Bacillus subtilis MES 810 is preserved in the China General Microorganism Culture Preservation Management Center, and the preservation address is No. 3, No. 1, Beichen West Road, Chaoyang District, Beijing, and the preservation date is As of August 10, 2017, the depo...

Embodiment 2

[0073] Bacillus subtilis and Bacillus amyloliquefaciens that adopt are identical with embodiment 1, and difference only is that the preparation technology of the fermented bacterium powder of Bacillus subtilis and Bacillus amyloliquefaciens is different, specifically as follows:

[0074] The preparation method of the compound microbial agent for preventing and controlling aquaculture cyanobacteria bloom of the present invention is: after the fermentation product of Bacillus subtilis (Bacillus subtilis) MES810 and the fermentation product of Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) MES812 are dried respectively, the dried fermented The bacteria powder is mixed in proportion to prepare the composite bacteria agent for preventing and controlling cyanobacteria bloom in aquaculture.

[0075] Wherein, (1) the fermentation bacterium powder preparation method of Bacillus subtilis is as follows:

[0076] ① Shake flask culture: The strain MES810 activated on the slant was...

Embodiment 3

[0096] Bacillus subtilis and Bacillus amyloliquefaciens that adopt are identical with embodiment 1, and difference only is that the preparation technology of the fermented bacterium powder of Bacillus subtilis and Bacillus amyloliquefaciens is different, specifically as follows:

[0097] The preparation method of the compound microbial agent for preventing and controlling aquaculture cyanobacteria bloom of the present invention is: after the fermentation product of Bacillus subtilis (Bacillus subtilis) MES 810 and the fermentation product of Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) MES 812 are dried respectively, dry The fermented bacteria powder is mixed in proportion to prepare the composite bacterial agent for preventing and controlling cyanobacteria bloom in aquaculture.

[0098] Wherein, (1) the fermentation bacterium powder preparation method of Bacillus subtilis is as follows:

[0099] ① Shake flask culture: The strain MES 810 activated on the slant was u...

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Abstract

The invention relates to a complex microbial inoculant for preventing and treating cyanobacterial bloom in aquaculture as well as a preparation method and an application of the complex microbial inoculant. The complex microbial inoculant comprises fermented Bacillus subtilis MES 810 powder and fermented Bacillus amyloliquefaciens MES812 powder in a mass ratio being 1:1. The complex microbial inoculant is prepared from the fermented Bacillus subtilis powder and the fermented Bacillus amyloliquefaciens powder by sufficient mixing in a mass ratio being 1:1, the viable count of the fermented Bacillus subtilis powder is not lower than 2.0*10<10>cfu / g, and the viable count of the fermented Bacillus amyloliquefaciens powder is not lower than 4.0*10<9>cfu / g. The prepared complex microbial inoculant for preventing and treating cyanobacterial bloom in aquaculture not only has an inhibition effect on cyanophyta algae, but also has no influence on other beneficial algae, thereby being an environment-friendly product.

Description

technical field [0001] The invention belongs to the technical field of microbial agent water quality adjustment, and in particular relates to a compound bacterial agent for preventing and controlling cyanobacteria bloom in aquaculture, a preparation method and application thereof. Background technique [0002] Oscillatoria, Phormidium, and Microcystis belong to Cyanophyta, Oscillatoraceae, Oscillatoria and Oscillatoria respectively, and are two types of algae that are harmful to water quality. Become the dominant algae and easily form cyanobacteria blooms. As a result, other algae cannot grow, and the pH value and ammonia nitrogen of the water body increase; when the cyanobacteria dies, the dissolved oxygen in the water body will drop sharply, and a large amount of cyanobacteria toxins will be produced, causing the death of farmed animals. Frequent outbreaks of cyanobacterial blooms have a huge impact on the ecological environment of water bodies and breeding benefits. The...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/125C12R1/07C02F101/16
CPCC02F3/34C02F2101/16C02F2101/166C12N1/20
Inventor 孙海远刘刚顾欣燕刘金龙肖培英赵晓琛
Owner 天津开发区坤禾生物技术有限公司
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