The invention discloses a formation and enrichment evaluation method for continental facies mud shale oil in a large freshwater lake basin. According to the method, the method comprises the steps of:utilizing shale oil exploration drilling coring, lithology and lithofacies accurate description and geological experiment matching analysis, carrying out formation and enrichment evaluation on continental facies shale oil; respectively adopting parameters indexes such as depth, Ro, diagenetic stage, kerogen, free oil quantity, the total porosity, organic cleavage seams, inorganic cleavage seams, areservoir evolution mode, a shale oil evolution mode, establishing a matching relationship among a continental facies shale oil formation and evolution mode, a reservoir stratum pore formation and evolution mode and a reservoir stratum evolution history and generation evolution history; and determining for the first time that a window and a secondary pore development zone which are favorable forformation of shale oil are formed in the late middle diagenetic stage Ro1.1%-1.6%, so that necessary conditions are created for large-scale aggregation of shale oil through high coupling in time and space, optimal selection and exploration breakthrough of the continental facies shale oil dessert in the Songliao basin are guided, the lower limit of the exploration depth can be expanded to 2,600 m,and the dessert area is expanded to 13,000 km<2> from 5,800 km<2>.