A highly lytic Salmonella phage rdp-sa-19009 and its application

Abstract

The invention discloses a highly lytic Salmonella phage RDP‑SA‑19009, the preservation number is CGMCCNo.21993, and the gene length is 87591bp. After sequencing the whole gene, it is found that it does not have virulence genes and lysogenic genes; Under the high temperature condition of ℃ for 30min, the titer remains at 1.0×10 6 More than pfu / mL, it has higher tolerance to temperature; the Salmonella phage RDP-SA-19009 provided by the present invention can crack including Salmonella typhimurium, Salmonella Stanley, Salmonella enteritidis, Salmonella Kentucky, Salmonella typhi, Salmonella Shenfudenburg Pathogenic bacteria, including Salmonella, have the characteristics of wide lysis spectrum and high lysis rate. The phage can be used alone or in combination with other Salmonella phages and probiotics, which can effectively treat and prevent poultry salmonellosis .

Description

A highly lytic Salmonella phage RDP‑SA‑19009 and its application technical field The present invention relates to the field of biotechnology, particularly relate to a wide cracking Salmonella pluvialis bacteriophage RDP-SA- 19009 and its use in pathogenic Salmonella infections in avian animals. Background technique Salmonellosis is one of the most important zoonotic diseases in public health, and it is a common food source. Sexual pathogens. Chickens are the most important host of Salmonella, Salmonella can cause acute or chronic diseases in chickens and infection with Salmonella The chickens can also lead to vertical transmission of Salmonella by laying infected eggs, thus forming a vicious circle. When chicken flocks break out Salmon Mortality rate can be as high as 80%, causing huge economic losses to the poultry industry. Once a person has ingested a large amount of Salmonella Bacterial animal food can lead to food-borne poisoning. According to statistics, a...

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a strain of wide lytic Salmonella phage RDP-SA-19009, aiming to solve the problems of acute onset of poultry due to Salmonella infection in farms and untimely treatment, and ineffective medication due to drug resistance of pathogenic bacteria.

Images