Bdellovibrio bacteriovorus for preventing and curing rice bacterial diseases and application thereof

A leech vibrio and bacterial technology, which is applied in the field of leech vibrio for preventing and treating bacterial diseases of rice, can solve the problem of lack of high-efficiency biocontrol strain preparations, etc., and achieve excellent elimination ability, rapid growth, and enhanced rice resistance. Effect

Inactive Publication Date: 2010-02-03
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In order to overcome the deficiencies in the current situation of the current situation of biocontrol strain preparations that do not have a high control effect against bacterial diseases of crops, especially bacterial diseases of rice, the primary purpose of the present invention is to provide a leech fungus for preventing and treating bacterial diseases of rice. bacteria

Method used

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  • Bdellovibrio bacteriovorus for preventing and curing rice bacterial diseases and application thereof
  • Bdellovibrio bacteriovorus for preventing and curing rice bacterial diseases and application thereof
  • Bdellovibrio bacteriovorus for preventing and curing rice bacterial diseases and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] (1) Obtain Bdellovibrio BDFM05 by ultraviolet mutagenesis method

[0039] The starting strain is Bdellovibrio BDS02. BDS02 was isolated from mud samples in our laboratory, and was deposited in the Chinese Type Culture Collection Center of Wuhan University in Wuhan, China on August 7, 2009, with the preservation number CCTCC NO : M 209170; After negative staining, BDS02 was observed under the electron microscope, showing a single cell, arc-shaped, with a size of 0.65×0.2μm, terminal flagella, and the length of the flagella was at least 1.8μm; Cultivate at 28°C for four days to form transparent circular plaques with a diameter of 1-2mm;

[0040] According to the application number "2008100220488.3" of Bdellovibrio BDS02, the method in the national invention patent application titled "Ultraviolet-induced mutation in enhancing the lysis performance of Bdellovibrio" was used, and the power of the ultraviolet lamp was 15W, and the power of the auxiliary infrared light source ...

Embodiment 2

[0053] (1) Preparation of bacterial agents for preventing and treating bacterial diseases of crops

[0054] Inoculate Aeromonas hydrophila (Aeromonas hydrophila, purchased from the Culture Collection Center of Guangdong Institute of Microbiology, No. GIM1.172) in LB liquid medium, culture at 26 ° C for 20 h, and the culture liquid was centrifuged and concentrated at 7000 rpm for 10 min. The precipitate was added to the DNB liquid medium, inserted into Bdellovibrio BDFM05, cultured at 28°C for 40 hours, the culture solution was centrifuged at 8000rpm for 18min, and the supernatant was centrifuged at 16000rpm for 20min, and the precipitate was suspended with an appropriate amount of normal saline to prepare into a concentration of 10 8 pfu / L of Bdellovibrio swimming body bacteria liquid.

[0055] Add agar oligosaccharide to the obtained bdellovibrio swimming body bacterial liquid to make the concentration 20g / L, mix well, and obtain the preparation for preventing and treating b...

Embodiment 3

[0062] (1) Preparation of bacterial agents for preventing and treating bacterial diseases of crops

[0063] Inoculate Aeromonas hydrophila (Aeromonas hydrophila, purchased from the Culture Collection Center of Guangdong Institute of Microbiology, No. GIM1.172) in LB liquid medium, culture at 30 ° C for 20 h, and the culture liquid was centrifuged and concentrated at 5000 rpm for 20 min, then added Into phosphate buffer (0.2mol / L, pH7.2~7.6), insert Bdellovibrio BDFM05, culture at 28°C for 42 hours, the culture solution is the mixed bacterial solution of hiruplasma and swimming body, and the hiruplasma The concentration ratio of body and swimming body is 1:1, and the concentration of bacteria solution is 10 10 pfu / L.

[0064] Add chitosan oligosaccharide and agarose oligosaccharide to the obtained bacterial liquid to make the concentration respectively 15g / L, mix well, and obtain the preparation for preventing and treating bacterial diseases of rice.

[0065] (2) The bacteria...

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Abstract

The invention discloses a bdellovibrio bacteriovorus for preventing and curing rice bacterial diseases and application thereof. The invention can obtain bdellovibrio bacteriovorus BDFM05 by separationand ultraviolet mutation. The bdellovibrio bacteriovorus BDFM05 is combined with oligosaccharide to obtain bdellovibrio bacteriovorus for preventing and curing crop bacterial diseases. The inventionapplies bdellovibrio bacteriovorus BDFM05 to prevent and cure crop bacterial diseases for the first time. The bdellovibrio bacteriovorus prepared by the method has better rice bacterial diseases preventing and curing effect than common Saisentong, has no toxicity or pollution, is safe to natural enemies of crop diseases and pests, produces no pathogenic bacteria resistance, has no toxic or side effects to people and animals, improves the quality and safety of agricultural products and conforms to the requirements of people on healthy and green food and sustainable-development for agriculturalproduction.

Description

technical field [0001] The invention belongs to the field of biotechnology, in particular to a Bdellovibrio for preventing and treating bacterial diseases of rice and its application. Background technique [0002] Bacterial diseases of crops are common worldwide epidemic diseases, many of which are listed as plant quarantine diseases, which are more serious and difficult to control than fungal diseases. With global climate anomalies and local warming, typhoons, rainstorms and thermals occur year by year, creating external climatic conditions for the aggravation of bacterial diseases. With the reduction of cultivated land, the multiple cropping index and the multiple cropping area are increasing, and a large amount of cultivated land cannot be fallow and rotated, and the accumulation of pathogenic bacteria and pathogenic bacteria base in the soil is gradually increasing. [0003] Bacterial diseases of rice are the main diseases in the growth process of rice, among which bact...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/02A01P1/00C12R1/63
Inventor 蔡俊鹏韩晓宁
Owner SOUTH CHINA UNIV OF TECH
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