Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Serum-free anaerobic high-density fermentation culture process for Streptococcus equi subsp. zooepidemicu

A high-density fermentation, Streptococcus equi technology, applied in the field of agricultural microorganisms, can solve problems such as vaccine safety hazards, and achieve the effects of improving immunogenicity, easy control of process parameters, and process stability

Active Publication Date: 2016-09-07
山东滨州沃华生物工程有限公司
View PDF2 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Streptococcus equi subspecies zooepidemicus is a facultative anaerobic microorganism that requires serum and glucose as nutrients during the growth process, but the residue of these heterologous substances will pose a safety hazard to the vaccine

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Serum-free anaerobic high-density fermentation culture process for Streptococcus equi subsp. zooepidemicu
  • Serum-free anaerobic high-density fermentation culture process for Streptococcus equi subsp. zooepidemicu

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] A serum-free anaerobic high-density fermentation process for Streptococcus equi subspecies zooepidemicus, comprising the steps of:

[0025] 1) Take the freeze-dried Streptococcus equi subsp. zooepidemicus strain ST171 fermented seed bacteria and activate them in TSA medium supplemented with 5% adult bovine serum for 12-16 hours until a single colony grows;

[0026] 2) Pick a single colony to the slant of TSA agar supplemented with 5% adult bovine serum, and cultivate it for 12-16 hours to obtain first-class seeds;

[0027] 3) Wash the slant of TSA agar supplemented with 5% adult bovine serum with 2 mL of TSB liquid, inoculate the primary seeds on the TSB medium supplemented with 5% adult bovine serum, and shake on a shaker at 37°C and 100rpm Cultivate for 3-4h until OD 600 When the value reaches 0.4-0.6, the secondary seed is obtained;

[0028] 4) Transfer the secondary seed solution to the fermentation medium (enriched casein medium) in a 10L fermenter with an inocul...

Embodiment 2

[0030] A serum-free anaerobic high-density fermentation process for Streptococcus equi subspecies zooepidemicus, comprising the steps of:

[0031] 1) Take the freeze-dried Streptococcus equi subsp. zooepidemicus strain ST171 fermented seed bacteria and activate them in TSA medium supplemented with 7% adult bovine serum for 12-16 hours until a single colony grows;

[0032] 2) Pick a single colony to the slant of TSA agar supplemented with 7% adult bovine serum, and cultivate it for 12-16 hours to obtain first-class seeds;

[0033] 3) Wash the slant of TSA agar supplemented with 7% adult bovine serum with 3 mL of TSB liquid, inoculate the primary seeds on the TSB medium supplemented with 7% adult bovine serum, and shake on a shaker at 38°C and 100rpm Cultivate for 3-4h until OD 600 When the value reaches 0.4-0.6, the secondary seed is obtained;

[0034] 4) Transfer the secondary seed solution to the fermentation medium (enriched casein medium) in a 15L fermenter with an inocul...

Embodiment 3

[0036] A serum-free anaerobic high-density fermentation process for Streptococcus equi subspecies zooepidemicus, comprising the steps of:

[0037] 1) Take the freeze-dried Streptococcus equi subsp. zooepidemicus strain ST171 fermented seed bacteria and activate them in TSA medium supplemented with 5% adult bovine serum for 12-16 hours until a single colony grows;

[0038] 2) Pick a single colony to the slant of TSA agar supplemented with 5% adult bovine serum, and cultivate it for 12-16 hours to obtain first-class seeds;

[0039] 3) Wash the slant of TSA agar supplemented with 5% adult bovine serum with 2 mL of TSB liquid, inoculate the primary seeds on the TSB medium supplemented with 5% adult bovine serum, and shake on a shaker at 37°C and 100rpm Cultivate for 3-4h until OD 600 When the value reaches 0.4-0.6, the secondary seed is obtained;

[0040] 4) Transfer the secondary seed solution to the fermentation medium (enriched casein medium) in a 10L fermenter with an inocul...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses serum-free anaerobic high-density fermentation culture process for Streptococcus equi subsp. Zooepidemicu and belongs to the field of agricultural microbiology. Streptococcus equi subsp. Zooepidemicu fermented high-density antigens are obtained through the sequential steps of resuscitating lyophilized seeds with TSA, carrying out primary culture on a slant of TS agar, carrying out secondary seed culture on a TSB medium, and anaerobically fermenting in enriched trypsin-casein medium free of serum and glucose. Study on industrial enlarged production is carried out through the method of anaerobic fermentation culture without adding any serum or glucose, the content of fermented antigens is not reduced, three is no need for ventilation regulation and dissolved oxygen control during fermentation, the antigen content is high, process parameters are easy to control, and supplements are reduced; the process is stable and high in growth speed compared with the existing fermentation process and is easy for implementing large-scale high-density culture of Streptococcus equinus antigens; immunogenicity of a strain is improved, and protection for vaccines is improved.

Description

technical field [0001] The invention relates to the technical field of agricultural microorganisms, in particular to a serum-free anaerobic high-density fermentation culture process of Streptococcus equi subspecies zooepidemicus. Background technique [0002] Streptococcus equi subspecies zooepidemicus belongs to Group C Streptococcus according to Langer's classification. This bacterium can cause a variety of animal infections. The clinical symptoms are sepsis, meningitis, arthritis, etc., and it is generally endemic. With the development of intensive pig farming, streptococcal suis occurs frequently in China. Epidemiological surveys showed that, in addition to Streptococcus suis type 2, Streptococcus equi subspecies zooepidemicus was still the main pathogen of Streptococcus suis in various parts of China. At present, the main means of controlling the disease is still vaccines, mainly attenuated vaccines and inactivated vaccines. [0003] Streptococcus equi attenuated stra...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N1/20C12R1/46
Inventor 朱杰王楠牛成明董新荣
Owner 山东滨州沃华生物工程有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com