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Streptococcus equi subsp. Equi HLJ2018D-LX strain and application thereof in preparation of streptococcus equi subsp. Equi inactivated vaccine

A HLJ2018D-LX, Streptococcus equi technology, applied in vaccines, veterinary vaccines, bacteria and other directions, can solve the threats to the sustainable development of the donkey industry, hinder the development and spread of the donkey industry and other problems, achieve good immunogenicity and reduce morbidity. , good effect of cultivating characteristics

Active Publication Date: 2020-12-01
HARBIN VETERINARY RES INST CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The occurrence of the disease has caused serious indirect economic losses to the donkey industry, and the disease is still spreading, which has a potential threat and a huge impact on the sustainable development of my country's donkey industry, seriously hindering the development of my country's donkey industry

Method used

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  • Streptococcus equi subsp. Equi HLJ2018D-LX strain and application thereof in preparation of streptococcus equi subsp. Equi inactivated vaccine
  • Streptococcus equi subsp. Equi HLJ2018D-LX strain and application thereof in preparation of streptococcus equi subsp. Equi inactivated vaccine
  • Streptococcus equi subsp. Equi HLJ2018D-LX strain and application thereof in preparation of streptococcus equi subsp. Equi inactivated vaccine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1 Isolation and culture identification of Streptococcus equi equi subspecies donkey-derived strain HLJ2018D-LX strain

[0026] According to the new biological product declaration requirement, in conjunction with a large amount of test data that the present invention obtains, with reference to " Chinese Veterinary Pharmacopoeia " (2005 edition), the poisonous species used for making seedlings has been identified, and the poisoned species standard of the trial procedure (draft) is now carried out as follows illustrate.

[0027] 1 Source of Poison Seeds

[0028] The HLJ2018D-LX strain of the present invention is obtained from the viscous purulent nasal fluid discharged from young foals caused by typical Streptococcus equi subsp. equi infection. Inoculate the nose swab of donkey foal infected with adenovirus by streaking on Columbia blood plate culture medium where typical β hemolysis occurs, such as figure 1 shown.

[0029] Inoculate the isolated and purified β-...

Embodiment 2

[0044] The preparation of embodiment 2 inactivated vaccines

[0045] 1 inactivation process

[0046] Formaldehyde solutions with final concentrations of 0.1% (v / v), 0.15% (v / v), and 0.2% (v / v) were selected for inactivation at 4, 28°C, and 37°C, and the results showed that 0.2% (v / v) Formaldehyde is inactivated at 28°C for 48 hours, and the inactivation effect is complete. Therefore, it is finally determined that 0.2% formaldehyde is inactivated at 28°C for 48 hours, coated with Columbia blood plate, cultured at 37°C for 3 days, and sterile, the inactivation is qualified, and then the inactivation is continued for 12 hours. Wash 2 times with PBS to remove formaldehyde.

[0047] 2 emulsification process

[0048] Use the commercialized MONTANIDE PET GEL A adjuvant from Sepic Company of France to mix and emulsify to make oil-in-water inactivated vaccine. Before emulsification, mix the same batch of inactivated Streptococcus equi subsp. Mixed at a ratio of 5%. Make up to volu...

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Abstract

The invention discloses a donkey-derived streptococcus equi subsp. Equi HLJ2018D-LX strain and application thereof in preparation of a streptococcus equi subsp. Equi inactivated vaccine, and belongs to the field of biological vaccines. The donkey-derived streptococcus equi subsp. Equi HLJ2018D-LX strain has the advantages of good immunogenicity, good culture characteristics and the like, and the inactivated vaccine is prepared by inoculating the streptococcus equi subsp. Equi HLJ2018D-LX strain into a streptococcus enrichment liquid, performing culturing, and performing inactivating with formaldehyde (the final concentration is 0.2% v / v). Experimental proves that the streptococcus equi subsp. Equi inactivated vaccine prepared by the invention is used for immunizing mice, the streptococcusequi subsp. Equi inactivated vaccine can prevent mice from being attacked by virulent streptococcus equi subsp. Equi, and greatly reduces the morbidity in donkey body tests. The vaccine has the advantages of safety, low-dose immunization and the like, is the first streptococcus equi subsp. Equi inactivated vaccine for donkeys, and can be used for preventing strangles caused by streptococcus equi subsp. Equi.

Description

technical field [0001] The present invention relates to a Streptococcus equi subspecies equi and its application in the preparation of Streptococcus equi subsp. Prepared inactivated vaccine. It belongs to the field of biological vaccines. Background technique [0002] Strangles is an acute contagious disease caused by Streptococcus equi subspeciesequi and the main symptom is swelling of the mandibular lymph nodes in equine animals. It is pathogenic to equine animals, and has various antigens such as hemolysin S, phospholipase A2, and surface antigen seM protein. Except for Iceland, glandular epidemics have occurred all over the world. Streptococcus equi subspecies mainly infects horses, followed by donkeys and mules. Horses from 4 months to 4 years old are susceptible, among which young foals under 2 years old are most affected, while foals within 2 months and horses over 5 years old are not easily infected. The disease mainly occurs in spring and autumn, usually from Se...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61K39/09A61P31/04C12R1/46
CPCA61K39/092A61P31/04A61K2039/521A61K2039/552C12R2001/46C12N1/205
Inventor 王晓钧郭巍胡哲戚亭刘荻荻
Owner HARBIN VETERINARY RES INST CHINESE ACADEMY OF AGRI SCI
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