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Method for screening candidate bacterial strain from fish streptococcus agalactiae vaccine

A technology for screening Streptococcus lactis and strains, which is applied in the direction of microorganism-based methods, biochemical equipment and methods, and microorganism measurement/inspection to achieve a high immune protection rate

Active Publication Date: 2014-02-26
GUANGXI INST OF FISHERIES
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Problems solved by technology

[0005] Aiming at the difficulties in research, development, production and application of fish streptococcus agalactiae vaccines, the main problem to be solved by the present invention is to provide a screening method for candidate strains of fish streptococcus agalactiae vaccines, which is used to guide the development, production and application of vaccines

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  • Method for screening candidate bacterial strain from fish streptococcus agalactiae vaccine
  • Method for screening candidate bacterial strain from fish streptococcus agalactiae vaccine
  • Method for screening candidate bacterial strain from fish streptococcus agalactiae vaccine

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Embodiment Construction

[0055] Take my country's tilapia streptococcus agalactiae vaccine candidate bacterial strain screening as example below, in conjunction with accompanying drawing, technical application of the present invention is described in detail:

[0056] 1. Isolation and preservation of prevalent strains of tilapia streptococcosis in my country

[0057] The epidemic strains of tilapia streptococcosis in my country were obtained by field isolation and laboratory isolation.

[0058] Field isolation: From 2006 to 2011, our laboratory personnel went to 12 cities in Zhanjiang, Maoming, Huazhou, Gaozhou, Yangjiang, Zhuhai, Yangchun, Gaoyao, Guangzhou, Qingyuan, Huizhou, Shantou in Guangdong Province, Nanning, 6 cities of Beihai, Yulin, Qinzhou, Baise, and Liuzhou, 5 cities of Haikou, Qionghai, Chengmai, Lingao, and Wenchang in Hainan Province, Zhangzhou City in Fujian Province, and Luoping County in Yunnan Province were isolated from streptococci on-site. The typical symptoms of streptococcal ...

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Abstract

The invention discloses a method for screening candidate bacterial strains from fish streptococcus agalactiae vaccines. The method comprises the steps: separation and breed conservation of tilapia streptococcicosis agalactiae epidemic strains, identification of the epidemic strains and the establishing of a pathogenic library, PFGE genotype analysis of epidemic strains, toxicity determination of PFGE genotype representative strains, and immunogenicity and protection domain test of the PFGE genotype representative strains so as to obtain a candidate bacterial strains combination of a tilapia streptococcicosis agalactiae immunoprophylaxis vaccine in China. The technology has the characterizes of strong pertinence, high screening efficiency, remarkable effect and the like, and the screen candidate bacterial strains combination of the tilapia streptococcosis agalactiae immunoprophylaxis vaccine in China can protect 90% of genotypes and 96.47% of epidemic strains in the pathogenic library. The technology provides a new method for screening the candidate bacterial strains from the fish streptococcus agalactiae vaccines, is suitable for screening the candidate bacterial strains from the fish streptococcus agalactiae vaccines, and has significance and using value in immune prevention and control on fish streptococcicosis.

Description

technical field [0001] The invention relates to a method for screening candidate strains of fish streptococcus agalactiae vaccine, in particular to the application of pulse field gel electrophoresis technology in the screening of candidate strains of fish streptococcus agalactiae vaccine. Background technique [0002] Streptococcus disease is one of the most serious diseases to fish farming at present, and it causes huge economic losses to the fish farming industry in the world every year. my country is a big aquaculture country, and the direct economic loss caused by streptococcal disease to aquaculture is about 1 billion US dollars every year. my country is the largest country in tilapia farming, and its annual output accounts for more than 50% of the world's total output. Since 2001, streptococcal infection has appeared in tilapia farming in China, and caused a high mortality rate of 30% to 50% in individual farms. For three consecutive years from 2009 to 2011, streptoc...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68C12Q1/14C12R1/46
Inventor 陈明王瑞李莉萍甘西梁万文黄婷雷爱莹罗永巨李健陈福艳
Owner GUANGXI INST OF FISHERIES
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