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Dual inactivated vaccine lymph for ascites disease of cultivated flounder and preparation method

A technology of inactivating bacteria and ascites disease, applied in antibacterial drugs, pharmaceutical formulas, bacterial antigen components, etc., can solve problems such as food safety, instability, and restrictions on the healthy development of the flounder aquaculture industry, so as to improve safety and avoid Harmful effect

Inactive Publication Date: 2009-04-22
天津市水产养殖病害防治中心
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The traditional treatment method is to use large doses of antibiotics to soak or take orally. The therapeutic effect is not obvious and unstable. The mortality rate of diseased fish is as high as 70%, and the economic loss is huge, which seriously restricts the healthy development of flounder aquaculture.
The long-term use of large doses of antibiotics not only fails to achieve good therapeutic effects, but also produces drug resistance while seriously polluting the environment. The residues of antibiotics in fish have also caused food safety problems, which has aroused people's great concern

Method used

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  • Dual inactivated vaccine lymph for ascites disease of cultivated flounder and preparation method
  • Dual inactivated vaccine lymph for ascites disease of cultivated flounder and preparation method

Examples

Experimental program
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Effect test

Embodiment 1

[0024] A dual inactivated bacterium vaccine for cultured flounder ascites disease, the preparation method of which is as follows:

[0025] (1) prepare inactivated bacterial vaccine of Edwardsiella tarda: activate Edwardsiella tarda bacterial strain (ATCC 15947 bacterial strain), inoculate in liquid culture medium (liquid culture medium is that 25gNaCl is added in 1 liter of nutrient broth culture medium to make) cultured culture medium) at 32°C to 37°C in an air bath shaker for 36 hours, terminate the culture when Edwardsiella tarda grows to the plateau stage, harvest, add formaldehyde, so that the final volume percentage concentration of formaldehyde is 0.5%, the temperature The temperature is 35°C, the inactivation time is 48 hours, shake well every 8 hours during this period, carry out the sterility test and formaldehyde residue test according to the "Chinese Veterinary Pharmacopoeia" sterility test and formaldehyde residue test, and the test is qualified, that is, the slow ...

Embodiment 2

[0037] A dual inactivated bacterium vaccine for cultured flounder ascites disease, the preparation method of which is as follows:

[0038] Edwardsiella tarda and Vibrio alginolyticus isolated from the kidney of flounder with ascites disease were purified by single colonies to obtain Edwardsiella tarda and Vibrio alginolyticus strains, which were respectively inoculated on ordinary nutrient agar plates (1 liter of ordinary nutrient agar Add 20g NaCl), culture in a 33°C-35°C bacterial incubator for 24h, wash the bacterial lawn with 0.85% sterile saline, vortex repeatedly to disperse the sticky bacteria, add an appropriate amount of formaldehyde to make the formaldehyde in the bacteria The final volume percent concentration in the liquid is 0.5%, inactivated at room temperature for 48 hours, and shaken every 8 hours. According to the sterility test and formaldehyde residue test method of "Chinese Veterinary Pharmacopoeia", the sterility test and formaldehyde residue test were car...

Embodiment 3

[0049] A double inactivated bacterium vaccine for cultured flounder ascites disease is prepared by the following method:

[0050] (1) Preparation of an inactivated vaccine of Edwardsiella tarda: activate the strain of Edwardsiella tarda, inoculate it in a solid medium, cultivate it at 32° C. to 35° C. for 48 hours, harvest when the Edwardsiella tarda grows to the plateau stage, Inactivate, carry out sterility test, make Edwardia tarda inactivated bacterin vaccine after passing the test, and described solid culture medium is 2216E culture medium;

[0051] (2) Preparation of inactivated Vibrio alginolyticus vaccine: activate the Vibrio alginolyticus strain, inoculate it in a solid medium, culture it at 28°C to 30°C for 48 hours, harvest when the Vibrio alginolyticus grows to the plateau stage, Inactivate, carry out sterility test, promptly make Vibrio alginolyticus inactivated bacterin vaccine after passing the test, and described solid culture medium is 2216E culture medium;

...

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Abstract

The invention discloses a flounder abdominal dropsy dual inactivated vaccine and a preparation method thereof. The flounder cultivating abdominal dropsy dual inactivated vaccine is prepared by the following method: (1) Edwardsiella tarda inactivated vaccine is prepared; (2) Vibro alginolyticus inactivated vaccine is prepared; and (3) the concentrations of the Edwardsiella tarda inactivated vaccine and the Vibro alginolyticus inactivated vaccine are adjusted to between 10<8> and 10<12> CFU / mL by physiological saline or 0.1 M sterile phosphate buffer the pH of which is 7.2 respectively, then the Edwardsiella tarda inactivated vaccine and the Vibro alginolyticus inactivated vaccine are mixed evenly according to the fact that the ratio of Edwardsiella tarda to Vibro alginolyticus is 1 to between 0.1 and 3, thereby preparing the flounder cultivating abdominal dropsy dual inactivated vaccine. The prepared vaccine can effectively prevent the occurrence and spread of the flounder abdominal dropsy, avoids the harm to the human body due to the residue of a large amount of antibiotics in fish bodies, improves the safety of cultivating products, and can not cause environmental pollution at the same time.

Description

technical field [0001] The invention belongs to the field of fish vaccines, and in particular relates to a dual inactivated vaccine for ascites disease of cultured flounder and a preparation method thereof. Background technique [0002] Farmed flounder (including turbot and brown flounder) is the main species of fish cultured in seawater industrialization in my country, and the aquaculture output accounts for more than 60% of the production of seawater industrialized fish farming, with significant economic benefits. With the expansion of the breeding scale, a kind of ascites disease appeared during the breeding process. The symptoms are: liver and kidney enlargement, abdominal distension, abdominal cavity filled with yellowish red liquid, and intestinal fluid accumulation. The traditional treatment method is to use large doses of antibiotics to soak or take orally. The therapeutic effect is not obvious and unstable. The mortality rate of diseased fish is as high as 70%, and ...

Claims

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Application Information

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IPC IPC(8): A61K39/116A61P31/04A61K39/02A61K39/106
Inventor 孙金生耿绪云王雪惠薛淑霞李翔董学旺
Owner 天津市水产养殖病害防治中心
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