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Breeding method of excellent strains of hybrid pelteobagrus fulvidraco resistant to Edwardsiella ictaluri

A technology of Wachener's yellow catfish and yellow catfish, applied in animal husbandry, etc., can solve the problems of mixed germplasm, supply shortage of YY super male fish, high price, etc., and achieve fast growth, huge economic and social benefits, Reduce the effect of using

Inactive Publication Date: 2019-11-19
NEIJIANG NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although all-male fish breeding has recently emerged across the country, the supply of YY super-male fish used in the production of all-male fry is tight and the price is high; and the germplasm is mixed in society, and the detection technology is only in the hands of a few people. It is difficult to carry out DNA detection and identification on all-male parent super-male male fish and all-male seedlings
Therefore, there are great limitations in the market

Method used

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  • Breeding method of excellent strains of hybrid pelteobagrus fulvidraco resistant to Edwardsiella ictaluri
  • Breeding method of excellent strains of hybrid pelteobagrus fulvidraco resistant to Edwardsiella ictaluri
  • Breeding method of excellent strains of hybrid pelteobagrus fulvidraco resistant to Edwardsiella ictaluri

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Embodiment Construction

[0022] The technical solution of the present invention is described in conjunction with the embodiments.

[0023] 1. Screening of populations of yellow catfish and yellow catfish resistant to catfish and Edwardsiella: use natural selection or artificial infection to screen populations of yellow catfish and yellow catfish that are resistant to catfish and Edwardsiella. according to figure 1 The specific procedures are as follows:

[0024] (1) Source collection of catfish Edwardsiella disease

[0025] ①Isolation and identification of bacteria

[0026] The pathogenic bacteria were isolated and purified according to the requirements of aseptic operation. Samples were taken from the liver, kidney and brain tissues of diseased yellow catfish, streaked on a BHI plate, cultured at a constant temperature of 28°C for 24-48 hours, picked dominant colonies with the same shape, and further streaked and purified to obtain pure culture strains, and then transformed into Received on the s...

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Abstract

The invention provides a breeding method of excellent strains of hybrid pelteobagrus fulvidraco resistant to Edwardsiella ictaluri.The method comprises the following steps: collecting the pelteobagrusfulvidraco and pelteobagrus vachelli, cultivating till mature, and then reproducing to obtain the fry of purebred pelteobagrus fulvidraco and the pelteobagrus vachelli with different populations; soaking all the fingerlings of the pelteobagrus fulvidraco and the pelteobagrus vachelli with a soaking method according to determined medial lethal concentration of the Edwardsiella ictaluri; performingbreeding on the population resistant to the Edwardsiella ictaluri twice, screening the excellent disease-resistant strains by the survival rate of mixed-cultured fry, respectively cultivating the strains of the pelteobagrus fulvidraco resistant to the Edwardsiella ictaluri, and the pelteobagrus vachelli to sexual maturity, and then producing the hybrid pelteobagrus fulvidraco according to a universal artificial reproduction method of fish. According to the breeding method of the excellent strains of the hybrid pelteobagrus fulvidraco resistant to the Edwardsiella ictaluri, hole-in-head-disease-resisitant parents of the pelteobagrus fulvidraco and the pelteobagrus vachelli can be cultivated, the incidence and mortality of hole-in-head disease in the seedling and culture process are improved, and the survival rate of the culture is improved.

Description

technical field [0001] The invention belongs to the technical field of fish breeding, and in particular relates to a method for breeding an excellent strain of hybrid yellow catfish resistant to Edwardsiella catfish. Background technique [0002] Selective breeding is a traditional fish breeding method, which uses the genetic variation of fish itself to isolate and retain excellent traits, quickly fix and develop excellent variations, and purify and rejuvenate fish species. At present, the theory of selective breeding, hybrid breeding, cell engineering breeding, molecular assisted breeding and genome selective breeding has been adopted at home and abroad. New species have been bred from farmed species such as tilapia, abalone, scallop, oyster, pearl oyster and meretrix meretrix. Using the fish sex control technology assisted by sex-linked genetic markers, the yellow catfish "Quanxiong 1", the all-female California perch "Northern flounder 1" and "Northern flounder 2", and t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/02
CPCA01K67/02
Inventor 覃川杰王均贺扬胡鹏史庆超吕云云
Owner NEIJIANG NORMAL UNIV
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