The invention discloses a nucleic acid combination for detecting pathogenic vibrio and its application, and relates to the detection technology of pathogenic vibrio. The nucleic acid combination includes one or more of the following primer pairs: SEQ ID NO.1-2, SEQ ID NO.3-4, SEQ ID NO.5-6, SEQ ID NO.7-8, SEQ ID NO.7-8, SEQ ID NO. ID NO.9-10, SEQ ID NO.11-12, SEQ ID NO.13-14, SEQ ID NO.15-16, SEQ ID NO.17-18 and SEQ ID NO.19-20; the nucleic acid combination Through the PCR method, it can detect common pathogenic Vibrio such as Vibrio cholerae, Vibrio parahaemolyticus, Vibrio vulnificus, Vibrio mimicus, Vibrio riverine, Vibrio alginolyticus, etc., to avoid non-specific amplification, with Strong specificity and high sensitivity.