The application discloses a method for identifying
pathogenic vibrio based on multiple LAMP technology and belongs to the technical field of
pathogen detection. The application overcomes the problem of mutual interference of multiple amplification primers, and a group of primer probe combinations suitable for a five-fold LAMP amplification
system are screened, which can simultaneously identify
Vibrio mimicus,
Vibrio fluvialis,
Vibrio alginolyticus, Vibrio metschnikovii and Vibrio furnissii. Further, the core detection
reagent is subjected to freeze-
drying treatment, is easy to store and transport, has high commercialization degree, and is convenient for primary or on-site detection. The technical scheme provided by the application has important significance for preventing and controlling the
outbreak of
pathogenic vibrio infection, tracing the source of epidemic diseases and
public health monitoring.