gi Genotype Norovirus Reverse Transcription Rolling Circle Amplification Method

Abstract

The invention relates to a GI genome type norovirus reverse transcription roll loop amplification detection method. A gene chip comprises a solid phase carrier and an oligonucleotide probe, wherein the oligonucleotide probe comprises one or more selected from the following nucleotide sequences: 1) DNA sequences selected from genes of vibrio hollisae, vibrio vulnificus, vibrio cholera, vibrio parahaemolyticus, vibrio harveyi, vibrio alginolyticus, vibrio furnissii, vibrio mimicus and vibrio damsel; 2) complementary DNA sequences of the DNA sequences selected from the 1); 3) complementary DNA sequences of the DNA sequences selected from 1) or 2). A kit comprises the gene chip. The gene chip and the kit provided by the invention are used for detecting nine pathogenic vibrios in ocean products, and are simple and convenient to operate, good in sensitivity and high in repeatability.

Description

technical field [0001] The invention belongs to the technical field of molecular biology virus detection, and in particular relates to a reverse transcription rolling circle amplification method for GI genotype norovirus. Background technique [0002] Zhejiang Province is a large marine province with a rich variety of seafood products. Seafood products are very popular among local people, and many residents have the habit of eating raw seafood. Seafood, especially shellfish, because of its filter-feeding characteristics, has become the carrier of food-borne viruses such as hepatitis A virus and norovirus, which may lead to the epidemic of human diseases. Seafood virus pollution generally refers to the virus enrichment pollution of shellfish. Usually, the virus is transmitted to humans after the water body is contaminated by viruses and then enriched by shellfish. So far, there is no specific drug to treat human viral diseases, and virus monitoring has become an effective me...

AI Technical Summary

Problems solved by technology

However, the PCR method is limited in application due to the influence of false positives and high detection cost; at the same time, there are many factors in the PCR method that affect the sensitivity and specificity, including the tested sample itself, the purification of viral nucleic acid, and the DNA fragment amplified by PCR. Less than 1kb cannot be used for whole genome amplification etc.

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