Detection method of vibrio vulnificus

Abstract

The invention discloses a detection method of vibrio vulnificus. The detection method comprises the following steps of A, detection sample making, B, gene extraction, C, 25ul reaction system preparing, D, multiplex fluorescence PCR, and E, data analysis. According to the detection method, the creative bacterial enrichment reagent technology, DNA extraction reagent technology, specific fluorescence probe hybridization PCR detection technology and multiplex PCR technology are adopted, no enriched culture is needed for samples, and false positive interference is reduced to the greatest extent on the premise of keeping high sensitivity. The detection method has the main beneficial effects that sample pre-enrichment is not needed, the bacterial detection sensitivity is high, specificity is good, and the false positive rate of conventional PCR amplification is reduced; and fast, accurate and specific detection on the vibrio vulnificus, vibrio alginolyticus, vibrio mimicus and vibrio fluvailis can be achieved at the same time.

Description

technical field [0001] The invention relates to a detection method, in particular to a detection method of Vibrio vulnificus. Background technique [0002] Vibrio vulnificus widely exists in the marine environment and is a common pathogenic Vibrio in mariculture animals. It mainly causes disease in eels, groupers, Chinese prawns, rainbow trout and other farmed animals, and can cause acute sepsis and tissue function decline in mariculture animals. Diseases such as fulminant, high mortality, extensive impact, once brought greater economic losses to marine animals. Vibrio vulnificus is also a pathogenic bacterium that is common to humans and animals. In recent years, it has caused several cases of acute septicemia in coastal areas such as the United States, South Korea, and Japan. It also has the characteristics of rapid onset, high mortality, and great harm. Widely concerned by the society, it is reported that most of the patients were injured during work or had acute infecti...

AI Technical Summary

Problems solved by technology

[0002] Vibrio vulnificus widely exists in the marine environment and is a common pathogenic Vibrio in mariculture animals. It mainly causes disease in eels, groupers, Chinese prawns, rainbow trout and other farmed animals, and can cause acute sepsis and tissue function decline in mariculture animals. and other diseases, with strong fulminance, high mortality rate and wide-ranging influence, which once brought great economic losses to seawater cultured animals.