Prepn and usage of extracellular product subunit vaccine of seawater fish morbid vibrio

A subunit vaccine and extracellular product technology is applied in the field of preparation of marine fish pathogenic Vibrio extracellular product subunit vaccine, which can solve the problems of large dosage, easy rebound, poor immune effect, etc., and achieve immune specificity. Stable and easy-to-use effects

Inactive Publication Date: 2007-08-22
GUANGDONG OCEAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The Vibrio anguillarum vaccine has poor immune effect due to the virulence that is easy to rebound, the dosage is large, and the body’s immune response level is low.
Minimal Vibrio conjugated subunit vaccines have specificity against minimal Vibrio in aquaculture production, and have poor defense against diseases caused by other Vibrio pathogenic bacteria, which has obvious limitations in production and application

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Vibrio alginolyticus and Vibrio harveyi were first activated with trypticase soytone liquid medium (TSB), cultured at 28°C with shaking at 150r / min for 18h, and then 1mL of the bacterial solution was spread on sterile cellophane The tryptone soy agar plate medium (TSA) containing 2% NaCl and the initial pH of 7.2 was cultured statically at 28° C. for 18 h. Then, extracellular products of Vibrio alginolyticus and Vibrio harveyi were extracted respectively. The method is as follows: wash the bacterial liquid with PBS of pH 7.2, and centrifuge at 10,000 r / min for 30 min at 4°C. The supernatant was filtered through a microporous membrane with a pore size of 0.22 μm to obtain Vibrio extracellular products (ECP). Determine the protein concentration in the samples with a UV spectrophotometer. Next, the extracellular product subunit vaccines of Vibrio alginolyticus and Vibrio harveyi were prepared respectively. The method is as follows: add 0.1% formalin to the extracellular...

Embodiment 2

[0037] Vibrio alginolyticus, Vibrio harveyi and Vibrio vulnificus were first activated with trypticase soytone liquid medium (TSB), cultured at 28°C with shaking at 150r / min for 18h, and then 1mL of the bacterial solution was spread on the shop floor respectively. Trypticase soy agar plate medium (TSA) containing 2% NaCl and an initial pH of 7.2 with sterile cellophane was cultured statically at 28°C for 18 hours. Then, extracellular products of Vibrio alginolyticus, Vibrio harveyi and Vibrio vulnificus were extracted respectively. The method is as follows: wash the bacterial liquid with PBS of pH 7.2, and centrifuge at 10,000 r / min for 30 min at 4°C. The supernatant was filtered through a microporous membrane with a pore size of 0.22 μm to obtain Vibrio extracellular products (ECP). Determine the protein concentration in the samples with a UV spectrophotometer.

[0038] Next, the extracellular product subunit vaccines of Vibrio alginolyticus, Vibrio harveyi and Vibrio vulni...

Embodiment 3

[0040] Vibrio alginolyticus, Vibrio harveyi, Vibrio vulnificus and Vibrio anguillarum were first activated with trypticase soytone liquid medium (TSB), cultured at 28°C with shaking at 150r / min for 18h, and then 1 mL of the bacterial liquid was taken separately Spread on tryptone soy agar plate medium (TSA) containing 2% NaCl and initial pH 7.2 covered with sterile cellophane, and cultured statically at 28°C for 18h. Then, the extracellular products of Vibrio alginolyticus, Vibrio harveyi, Vibrio vulnificus and Vibrio anguillarum were extracted respectively. The method is as follows: wash the bacterial liquid with PBS of pH 7.2, and centrifuge at 10,000 r / min for 30 min at 4°C. The supernatant was filtered through a microporous membrane with a pore size of 0.22 μm to obtain Vibrio extracellular products (ECP). Determine the protein concentration in the samples with a UV spectrophotometer. Next, the extracellular product subunit vaccines of Vibrio alginolyticus, Vibrio harvey...

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PUM

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Abstract

The present invention is preparation and usage of extracellular product subunit morbid vibrio vaccine for seawater fish. The vibrio spawn is two or more of Vibrio alginolyticus, Vibrio Hrveyi, Vibrio vulnificus, Vibrio parahaemolyticus, etc. The vaccine preparing process includes culturing vibrio, extracting extracellular product and preparing vaccine. The vaccine containing partial pathogen and no infecting component has stable immunological specificity and no hidden danger of restoring toxici. The vaccine is used in preventing fishes' skin ulcer, eyeball turbidity and other vibrio caused diseases and may be used widely for immunizing seawater fish.

Description

Technical field: [0001] The invention relates to a method for preparing and using a seawater fish pathogenic Vibrio extracellular product subunit vaccine. Background technique: [0002] In recent years, with the rapid development of the mariculture industry, the breeding density has increased, the environment of the aquaculture waters has gradually deteriorated, and the occurrence of diseases has become more and more frequent. Bacterial disease has become one of the most common and most harmful diseases in aquaculture, often leading to a large number of deaths of farmed fish, among which Vibrosis (Vibrosis) is the most common and most harmful disease in seawater fish farming. Fish farming causes huge economic losses. [0003] For a long time, traditional control methods have generally used chemical drugs and antibiotics. Extensive use of these drugs can easily cause pathogenic bacteria to develop drug resistance, destroy the micro-ecosystem of aquaculture water, and cause d...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/106A61P31/04
Inventor 简纪常庞欢瑛左凤琴吴灶和
Owner GUANGDONG OCEAN UNIVERSITY
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