Detection kit and detection method for 5 new pathogens in foods

Abstract

The invention relates to a detection kit and a detection method for 5 new pathogens in foods. The kit comprises 5 detection solutions each of which contains 10 mm of Tri*Cl, 50 mm of KCl, 25 mm of MgCl2, 2.5 mm of each dNTP component and 5U/microlitre of DNA polymerase. Besides, the detection solution A, B, C, D or E respectively contains specific primer pairs of Laribacter hongkongensis, Serratiamarcescens, Serratia odorifera, Plesiomonas shigelloides or Vibrio mimicus, each of which is 10 micrometers in amount. The detection kit and the detection method can detect the Laribacter hongkongensis, the Serratia marcescens, the Serratia odorifera, the Plesiomonas shigelloides and the Vibrio mimicus at a high sensitivity, have little detection time and simple operation, save large labor and financial resources, and meet the requirements of the quick detection.

Description

technical field [0001] The present invention relates to a kit for the detection of 5 newly emerging pathogenic bacteria in food, i.e. Hong Kong gull-type bacteria, Serratia marcescens, Serratia odorifera, Pseudomonas shigella-like and Vibrio meeserii And a detection method, in particular to a PCR-DHPLC-based rapid detection kit and detection method. Background technique [0002] Food microorganisms are widely found in various foods such as aquatic products, meat products, and dairy products. The research on rapid detection technology for microorganisms is the direction that food safety workers have been working hard for. Judging from literature reports, people's research mainly focuses on several common microbial items, such as Salmonella, Listeria monocytogenes, etc. Gulliformis, Proteus vulgaris, Proteus mirabilis, Serratia marcescens, Serratia odoriferus, etc. are relatively less studied, and little is known about their existence in food and their disease hazards to huma...

AI Technical Summary

Problems solved by technology

[0008] At present, rapid detection technologies for food microorganisms mainly include: PCR-based gel electrophoresis, real-time fluorescent PCR, fluorescent immunoassay (VIDAS), colloidal gold immunoassay strips and gene chip testing, etc. There are respective advantages and disadvantages and limitations in the scope of application

PCR electrophoresis technology has the problems of cumbersome operation steps and easy to cause pollution. At present, no PCR-based technology has been used to detect Vibrio melini, P. bacteria, Proteus vulgaris, Proteus mirabilis, Serratia marcescens, and Serratia odoriferous; real-time fluorescent PCR technology has the problems of high testing cost and small range of target bacteria that can be detected; Fluorescent immunoassay technology (VIDAS) technology has the problems of high test cost and a small range of target bacteria that can be detected; colloidal gold immunoassay technology has the problems of high false positive rate and a smaller range of target bacteria that can be detected; gene chips The detection range of technology detection technology is also very limited, and the test cost is high, and there are still unresolved technical bottlenecks before the actual application

The use of traditional microbial detection methods is time-consuming and complicated, and it is not suitable for the detection of large-scale samples. In other aspects such as food and cosmetics that require large-scale testing, traditional detection methods have exposed their various drawbacks

In clinical practice, the long detection time is more likely to lead to delayed diagnosis and treatment

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