Kit for detecting equine piroplasmosis and preparation method and using method thereof

Abstract

The invention discloses a detection kit for identifying and diagnosing whether an equine animal is infected with piroplasmosis or not, and a preparation method and a using method thereof. A detection film for detecting equine piroplasmosis and a piroplasmosis universal primer are arranged in the kit for detecting the equine piroplasmosis. The using method of the kit comprises the following steps:extracting DNA (deoxyribonucleic acid) from blood of a horse to be detected, further using the piroplasmosis universal primer to perform amplification, combining an amplification product with the detection film, enabling biotin at the terminal of a PCR (polymerase chain reaction) product to be in action with streptavidin-peroxidase, and then playing a catalysis role in enabling a substrate of peroxidase, namely an enhanced chemiluminescence reagent (ECL-detection) to emit fluorescence, thereby exposing an X-photosensitive film; and performing development and fixing treatment on the film so asto emerge a shadow in a region corresponding to specificity hybridization of a probe on the detection film and the PCR product, and further judging whether the detected animal is infected with the piroplasmosis or not according to whether the shade is left on the film or not.

Description

technical field [0001] The invention relates to a molecular biology identification and detection technology of animal parasites, specifically, the invention provides a detection kit for differential diagnosis of whether equine animals are infected with piriformis and a preparation and use method thereof. Background technique [0002] Equine piroplasmosis is an acute, subacute or chronic protozoal parasite caused by Babesia solani and Theileria equi parasitic in the red blood cells of equines (horses, donkeys, mules and zebras). The disease, which is transmitted by ticks, is mainly characterized by causing animal fever (sometimes periodically), anemia, jaundice, and hepatosplenomegaly. In the later stage of the disease, animals often have symptoms such as bilirubinuria and hemoglobinuria. Babesia caballi Nutall et Strickland, 1910 (old name horse coke, proplasma caballi) is a sub-kingdom of Protista (Protozoa), Apicomplexa, Sporozoea (Sporozoea) A member of the genus Babesia...

AI Technical Summary

Problems solved by technology

At present, the detection and diagnosis of equine piriplasmosis mainly include the following types of means: (1) Traditional methods such as blood smear staining microscope detection, lymph node puncture test, in vitro culture, clinical diagnosis and animal inoculation test. The rate is low, and the requirements for the quality of testing personnel are very high

Images