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Vibrio mimicus chromogenic culture medium

A technology of chromogenic medium and mimetic Vibrio, applied in the field of food hygiene and microbial safety monitoring, can solve problems such as low specificity, and achieve the effects of overcoming poor selectivity, shortening detection time, and reducing workload

Inactive Publication Date: 2009-10-14
ZHEJIANG GONGSHANG UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In view of the above situation, in order to overcome the defect of low specificity of the existing Vibrio mimicus selective medium, the present invention provides a highly specific chromogenic medium for Vibrio mimicus, which can quickly detect Vibrio mimicus

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Embodiment 1: Verification of the specificity of Vibrio mimicus chromogenic medium according to the present invention

[0017] 1. Preparation of Vibrio mimicus chromogenic medium: take 5g of yeast extract powder, 5g of fish peptone, 5g of peptone, 10g of sodium chloride, 10g of sodium citrate, 10g of sodium thiosulfate, 3g of sodium cholate, and 5g of ox gall powder , 1g of ferric citrate, 15g of agar, 1000mL of distilled water, heated and stirred to dissolve, boiled for 1-2min, cooled to 50-55°C, added 20g of sucrose, 20g of cellobiose, 20g of arabinose, bromothyme Phenol blue 0.04g, thymol blue 0.04g, shake well, adjust the pH to 8.6±0.2 with 1mol / L NaOH, pour the culture medium into the plate, cool it and place it upside down in a 15°C incubator for later use.

[0018] 2. Vaccination: Vibrio mimeticus, Vibrio alginolyticus, Vibrio parahaemolyticus, Vibrio vulnificus, Vibrio cholerae non-O1, Vibrio cincinnati, Vibrio riverine, Vibrio meeseri, Vibrio furnisis, Vibrio m...

Embodiment 2

[0020] Embodiment 2: Verification of the plate efficiency of Vibrio mimicus chromogenic medium plate according to the present invention

[0021] 1. Preparation of Vibrio mimicus chromogenic medium: take 5g of yeast extract powder, 5g of fish peptone, 5g of peptone, 10g of sodium chloride, 10g of sodium citrate, 10g of sodium thiosulfate, 3g of sodium cholate, and 5g of ox gall powder , 1g of ferric citrate, 15g of agar, 1000mL of distilled water, heated and stirred to dissolve, boiled for 1-2min, cooled to 50-55°C, added 20g of sucrose, 20g of cellobiose, 20g of arabinose, bromothyme Phenol blue 0.04g, thymol blue 0.04g, shake well, adjust the pH to 8.6±0.2 with 1mol / L NaOH, pour the culture medium into the plate, cool it and place it upside down in a 15°C incubator for later use.

[0022] 2. Vaccination:

[0023] After resuscitating the standard Vibrio mimetic strain on 3.5% NaCl nutrient agar for 24 hours, pick 1 loop from the inoculation loop, add it to 10mL 0.85% normal s...

Embodiment 3

[0032] Embodiment 3: Verification of the accuracy and sensitivity of Vibrio mimicus chromogenic medium according to the present invention

[0033] 1. Preparation of Vibrio mimicus chromogenic medium: take 5g of yeast extract powder, 5g of fish peptone, 5g of peptone, 10g of sodium chloride, 10g of sodium citrate, 10g of sodium thiosulfate, 3g of sodium cholate, and 5g of ox gall powder , 1g of ferric citrate, 15g of agar, 1000mL of distilled water, heated and stirred to dissolve, boiled for 1-2min, cooled to 50-55℃, added 20g of sucrose, 20g of cellobiose, 20g of arabinose, and musk bromide Shake 0.04g of grass mol blue and 0.04g of thymol blue, shake well, adjust the pH to 8.6±0.2 with 1mol / L NaOH, pour the culture medium into the plate, cool it and place it upside down in a 15°C incubator for later use.

[0034] 2. Vaccination:

[0035] (1) Samples contaminated by pure bacteria: resuscitate Vibrio mimicus MCCC 1H00078 (the strain number of the China Marine Microorganism Cul...

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Abstract

The invention discloses a vibrio mimicus chromogenic culture medium with the following formula: 5g of yeast extract powder, 5g of fish peptone, 5g of peptone, 10g of sodium chloride, 8g to 10g of sodium citrate, 8.5g to 11g of sodium thiosulfate, 1g to 5g of sodium cholate, 2g to 7g of ox-bile powder, 15g to 20g of sucrose, 15g to 20g of cellobiose, 15g to 20g of arabinose, 1g to 3g of ironic citrate, 12g to 20g of agar, 0.04g of bromothymol blue, 0.04g of thymol blue and 1000mL of distilled water. The invention designs the vibrio mimicus chromogenic culture medium which is obviously different from other bacteria, especially other vibrio or other food pathogenic bacteria, according to the basic nutritional need, the specific ecological environment, the specific biochemical reaction, namely, an enzyme system and the metabolization rule, and the toleration to specific bacteria-resistant substances and the like of the vibrio mimicus, overcomes the defects of poor selectivity and the like of the traditional culture medium, and can quickly detect the vibrio mimicus in marine food products.

Description

technical field [0001] The invention relates to a microbial chromogenic culture medium, which belongs to the field of food sanitation microbial safety monitoring. Background technique [0002] Among the hundreds of millions of food-borne disease patients in the world every year, 70% are caused by eating food and drinking water contaminated by various pathogenic microorganisms. Vibrio mimicus (Vibrio mimicus) is a Gram-negative Vibrio with a single flagella and power. Like Vibrio parahaemolyticus and Vibrio cholerae, it is a normal marine bacterium and a low-grade halophilic bacterium. It is widely distributed in natural rivers, In seawater and seafood. Vibrio mimicus is one of twelve species of pathogenic Vibrio. It can cause diarrhea, otitis media, trauma infection and vibriosis in aquatic organisms in humans, cause severe ascites disease in fish, crustaceans and other aquatic animals, and can also infect humans through aquatic animals or aquatic products, causing intesti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/04G01N21/78C12R1/63
Inventor 赵广英申科敏
Owner ZHEJIANG GONGSHANG UNIVERSITY
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