83 results about "Thymol blue" patented technology

The invention relates to the field of food detection, and relates to a preparation method of intelligent indicator labels for freshness of meat, and application, and the preparation method comprises the following steps of firstly preparing a fluorescent carbon quantum dot, dissolving the fluorescent carbon quantum dot in ethanol with bromothymol blue, and adjusting the pH value to obtain a freshness indicator; mixing the chitosan solution, polyvinyl alcohol solution and freshness indicator, forming an indicator film after being de-foamed and dried by ultrasound, and wrapping with a low densitypolyethylene film to obtain intelligent freshness indicator labels; recording the visible light color pictures of the labels for different storage days after coexisting the labels with the meat to form a visible light colorimetric card, and recording the fluorescent color pictures to form a fluorescent color colorimetric card; and when the colors of the labels change after the meat to be tested and the labels coexist, realizing the visual detection of the freshness of the meat through the color comparison of the colorimetric cards. The labels prepared by the invention are safe and environmentally friendly, and the fluorescent nano-materials combined with the traditional color indicators make the color signals of the indicators more abundant and the measurement results more accurate.

The invention relates to the technical field of fish detection, and particularly relates to fish freshness detection test paper. Each liter of an indicator solution in a reagent layer contains 0.5-1g of bromophenol red, 0.6-1.1g of bromcresol purple, 0.3-0.5g of bromothymol blue, 0.3-0.8g of neutral red, 0.2-0.6g of phenol red and the balance of a solvent. The fish freshness is detected rapidly with low cost and high flexibility. The detection test paper can be used for detecting the freshness of chilled, refrigerated and frozen fish. The using method is simple; and only less amount of fish body surface mucus is dipped when the test paper is used, and the fish freshness can be obtained by comparing the color instantly developed by the test paper with a standard color chart.

The invention relates to a piece of highly sensitive pH test paper and a preparation method thereof, wherein a developing strip is in the form of a piece of pH body paper, which is dipped in a reagent solution and then dried by hot air at constant temperature; the reagent solution contains an indicator; the formula of the indicator contains bromothymol blue, thymol blue, metanil yellow, m-methyl red, bromocresol green, nitrazine yellow, cresol red, phenolphthalein, para-nitrophenylazosalicylate sodium, titan yellow, fluorescent pigment orange red, 5% 4-nitrobenzenediazo-1-naphthol-3.6-sodium disulfonate solution and 30% dissolved ethanol aqueous solution; and the formula of the indicator further contains an aqueous colour fixing agent, a cotton wooden fibre mildew preventive, a special paper mildew preventive and a buffer neutralizer formed by modulating sodium hydroxide and phosphoric acid. The preparation method comprises the following steps of: adjusting the reagent solution till a pH value is up to 6-7.5, uniformly wetting the pH body paper, and drying through hot air at constant temperature. According to the invention, the reagent solution can be adjusted, so that the pH value is up to 6-7.5; steady and precise high sensitivity of the pH1-14 test paper is effectively increased; and the test paper is convenient and simple to use.

The invention provides a humidity indicator which is really effective in environmental protection and comprises a carrier and solution used for soaking the carrier; wherein, the solution takes 30-80% of ethanol as a solvent and contains 0.00862-0.00992% of acid-base indicator and 0.8-13.8% of hygroscopic salt. The acid-base indicator is one of bromocresol green, bromothymol blue, thymol blue, thymolphthalein, bromophenol blue, methyl red, methyl orange, methyl yellow , neutral red and phenol red or the mixture thereof. The hygroscopic salt is alkali halide or alkali-earth metal halide. The humidity indicator of the invention has wide indicating range (capable of indicating 5-90% of ambient humidity) and high sensitivity. Diverse humidity indicators different in discoloration can be manufactured by adjusting the components. The humidity indicator of the invention has evident discoloration effect, simple use, reutilization, good suitability and extensive application.

The invention relates to a rice metabolism detecting reagent and method, concretely used in the condition of detecting rice metabolism. It mainly takes and dissolves nitrazine yellow/chlorophenol red, p-nitrophenol, bromocresol green/bromothymol blue/resorcinol blue, bromocresol purple, and hematoxylin/cresol red/bromophenol red/methyl red into alcohol, and mixing them uniformly to obtain finished product of raw reagent liquid. As detecting, taking a raw reagent liquid and adding distilled water to dilute the raw reagent liquid so as to form a liquid reagent, adding rice to the liquid reagent to react, observing the colors of rice and solution, judging the metalolism degree of the rice detected, the metabolism situation and color of the rice corresponds to green and passes through yellow and salmon pink into red region, where the green expresses new rice, yellow expresses old rice and salmon pink expresses deeply aged rice. The reagent of the invention is lower-cost and has simple manufacturing process; the operation is quick, accurate and convenient; the detecting method is simple, able to be used in both qualitative detection and quantitative detection.

The invention discloses a hydraulic pressure welding seam fast leak detection agent, which contains constituents of (mass percent): ethanol of 65-70%, poly(vinyl butyral) of 0.13-0.18%, light magnesium oxide of 28.5-33.5%, thymol blue of 0.10-0.15%, and anti-precipitation agent of 1.22-1.5%. Preparation method for the leak detection agent includes steps: adding ethanol into a intermittent sand mill, actuating the sand mill, firstly adding poly(vinyl butyral), and then adding light magnesium oxide, thymol blue and anti-precipitation agent, grinding the mixer until no particle, and obtaining the leak detection agent. Advantages of the invention are: drying of coating is fast, adhesive force is good, color contrast is obvious, color change of water seepage in welding seam and outside spitting water can be differentiated, and estimation effect is fast and exact.

The present invention discloses a method for determination of magnesium content in aluminum alloy, including: dissolving an aluminum alloy sample, using one or more compounds selected from the group consisting of mercapto-containing compound, acetone cyanohydrin, β-aminoethyl mercaptan, triethanolamine, tetraethylenepentamine, ethylene diamine and oxydol as masking agent, using eriochrome black T or methyl thymol blue as indicator, and using EDTA or CDTA to titrate the sample.

The invention discloses an amniotic fluid detection indicator composition, preparation thereof and a corresponding amniotic fluid detection pad. The amniotic fluid detection indicator composition is used by taking one or two of water, ethyl alcohol, acetone and N,N-dimethylformamide as a solvent or solvents, taking one or more than one of pulullan, sodium alginate, ethyl cellulose and sodium carboxymethylcellulose as a film-forming agent or film-forming agents, taking dioctyl phthalate as a plasticizer, taking ethylene glycol, glycerol and glycol ethylene ether as wetting agents, taking bromothymol blue sodium salt, bromoxylenol blue, sodium alizarine sulfonate and bromcresol purple as indicators, preparing an amniotic fluid detection indicator at the normal temperature, then coating a medical sanitary pad with the amniotic fluid detection indicator, and drying the medical sanitary pad to prepare an amniotic fluid detection pad. The amniotic fluid detection indicator composition is convenient to use, not easy to decolor and more easily acceptable to patients, and is capable of distinguishing amniotic fluid and urine; the whole process is simple to operate; the large-scale production is easy to implement.

The invention relates to the technical field of medical treatment, in particular to a pancreatic leakage detection material based on acid-base indication compound chemical color changes and application thereof. The pancreatic leakage detection material is a water absorption material comprising a solution loaded with acid-base indication compounds, and is prepared by preparing a solution through acid-base indication compounds and dropwise adding the solution to the water absorption material. The acid-base indication compounds are thymol blue or a mixture of thymol blue and bromothymol blue. By means of the pancreatic leakage detection material, tissue liquid with pancreatic juice and tissue liquid without pancreatic juice can be distinguished through obvious color changes under the condition of low-dosage pancreatic juice, and the high detection flexibility and the large detection range are achieved.

The invention discloses a selectivity differential medium-vibrio alginolyticus selectivity differential medium (VaDM) which uses rapid screened vibrio alginolyticus as a unique target bacterium. The vibrio alginolyticus selectivity differential medium is prepared from leucine, yeast leaching powder, sodium chloride, sodium thiosulfate, sodium cholate, bile powder, ferric citrate, calcium chloride, agar, bromothymol blue, thymol blue and distilled water. In the invention, all components of the VaDM are optimized and combined according to a special leucine aminoase system of vibrio alginolyticus, basic and special nutrition requirement, halophilism and resistance on some bacteriostats, favorable growth of the bacterium is guaranteed, the growth of a plurality of other miscellaneous bacteriacan be effectively inhibited, the target bacterium presents special bacterial colony color and achieves good selecting and distinguishing effects with other bacteria. The invention verifies that VaDMhas an ideal effect on the rapid screening of vibrio alginolyticus by testing bacterium yield, accuracy, precision and sensitivity and comparing the conventional detecting method for detecting a practical sample.

The invention discloses a concrete carbonation depth measurement reagent and preparation and application methods thereof. The concrete carbonation depth measurement reagent is prepared from thymol blue, phenolphthalein, alizarin yellow R, sodium hydroxide, absolute ethyl alcohol and distilled water. In the components, the thymol blue, the phenolphthalein and the alizarin yellow R and alkaline substances with different pH values in carbonated concrete are correspondingly subjected to color reactions, and thus color development relations with obvious distinctions are obtained for the concrete with different degrees of carbonization, and the effect that the concrete carbonation depth is measured accurately is achieved. Color overlapping caused by different reagents is taken into account by adopting a color superposition principle; the sodium hydroxide, the absolute ethyl alcohol and the distilled water are taken as lytic agents of the thymol blue, the phenolphthalein and the alizarin yellow R; the protective agent is good in stability, is non-toxic and environment-friendly, is easy to manufacture and high in accuracy, can be widely used for depth measurement of various carbonated concrete, and rapidly measuring of the concrete carbonation depth is facilitated.

The invention discloses a vibrio mimicus chromogenic culture medium with the following formula: 5g of yeast extract powder, 5g of fish peptone, 5g of peptone, 10g of sodium chloride, 8g to 10g of sodium citrate, 8.5g to 11g of sodium thiosulfate, 1g to 5g of sodium cholate, 2g to 7g of ox-bile powder, 15g to 20g of sucrose, 15g to 20g of cellobiose, 15g to 20g of arabinose, 1g to 3g of ironic citrate, 12g to 20g of agar, 0.04g of bromothymol blue, 0.04g of thymol blue and 1000mL of distilled water. The invention designs the vibrio mimicus chromogenic culture medium which is obviously different from other bacteria, especially other vibrio or other food pathogenic bacteria, according to the basic nutritional need, the specific ecological environment, the specific biochemical reaction, namely, an enzyme system and the metabolization rule, and the toleration to specific bacteria-resistant substances and the like of the vibrio mimicus, overcomes the defects of poor selectivity and the like of the traditional culture medium, and can quickly detect the vibrio mimicus in marine food products.

The invention relates to a needle mushroom strain detection culture medium and a usage method thereof, and belongs to the technical field of edible fungi strain degeneration detection. The invention aims at solving the technical problem of providing a needle mushroom strain activity degradation detection method which has the advantages of short detection period and high accuracy and a detection culture medium formula. The technical scheme is as follows: the needle mushroom strain detection culture medium comprises the following raw materials in parts by weight: 100-250 parts of potatoes, 10-30 parts of lactose, 1-3 parts of NH4NO3, 1-3 parts of KH2PO4, 0.1-1.5 parts of MgSO4.7H2O and 0.01-0.5 part of bromothymol blue. The needle mushroom strain detection culture medium disclosed by the invention can be used for culturing the strains to be detected; and according to the invention, the strains with good activity can decolorize the culture medium, and degraded strains or inactivated strains enable the color of the culture medium to be shallow or unchanged.

The invention discloses pH test paper for water quality testing and a water quality testing method. The pH test paper for water quality testing is prepared by soaking a carrier in a pH color-developing reagent, and drying at 50 to 60 DEG C for 10 to 40 minutes, wherein the pH color-developing reagent is a bromine thymol blue solution with the concentration being 0.7 to 0.8g/L, a phenol red solution with the concentration being 0.7 to 0.8g/L, or a bromocresol green solution with the concentration being 0.5 to 0.6g/L; the carrier is a chromatography test strip or chromatography filter paper. According to the pH test paper for water quality testing and the water quality testing method provided by the invention, pH of water quality can be quickly, conveniently and accurately detected without the need for using a special instrument, so that a detection range is wide; a bromine thymol blue and phenol red indicator developing in an alkaline zone but not developing in an acid zone is selectedfor detecting an alkaline part, and a bromocresol green indicator developing in the acid zone but not developing in the alkaline zone is selected for detecting an acid part, so that a detection resultis more accurate; phenol red pH test paper prepared by a color-developing reagent phenol red can be also used for carrying out quantitive pH detection.

The invention discloses a vibrio test piece, which comprises a test piece body, a vibrio culture medium and a hydrogel carrier. The vibrio culture medium is prepared from the following components in percentage by mass: 2.2 to 3.5 percent of peptone, 1.5 to 2.0 percent of saccharose, 0.2 to 0.4 percent of ox gallbladder powder, 0.08 to 0.15 percent of sodium thiosulfate, 0.05 to 0.12 percent of ferric citrate, 0.002 to 0.005 percent of thymol blue, 1.0 to 1.5 percent of yeast, 0.8 to 1.0 percent of sodium chloride, 0.12 to 0.2 percent of deoxysodium cholate, 0.5 to 1.0 percent of trisodium citrate, 0.002 to 0.004 percent of bromothymol blue, 0.1 to 0.3 percent of xanthan gum, and the balance of pure water, wherein the pH value of the vibrio culture medium is 9.0 to 9.3. The invention also discloses a preparation method of the vibrio test piece. The vibrio test piece provided by the invention is convenient for a water body sample to absorb and diffuse in the culture medium, and providesa favorable growth environment for vibrio.

The invention relates to a preparation method of universal pH test paper. An indicator is prepared by dissolving Metanil yellow, m-methyl red, nitrazine yellow, titan yellow, alizarin yellow R, bromothymol blue, thymol blue, thymolphthalein, phenolphthalein, bromocresol green and o-cresolphthalein in a 30% ethanol water solution. The preparation method comprises the following steps: previously impregnating base paper of pH test paper in the prepared indicator solution, and drying with heating steam of 95-105 DEG C at normal pressure. The preparation method of universal pH test paper is simple in process, stable in product quality and higher in sensitivity and accuracy in comparison with the common universal test paper, and can be widely used in the fields of scientific research, industry, agriculture, environmental protection, medicine and food.

The invention provides a detection composition for detecting helicobacter pylori. The composition contains urea, dipotassium phosphate, sodium hydroxide, methyl p-hydroxybenzoate, bromothymol blue and distilled water. The invention also provides a kit containing the composition and used for detecting helicobacter pylori. The kit comprises an oral cavity sampling device, a detection device, an extrusion type plastic bottle filled with the composition and an extrusion type plastic bottle filled with 95% v/v ethanol. The kit has the following technical effects that the kit is simple and convenient to operate, is suitable for general consumers without professional knowledge and equipment, and can be used for accurately and quickly detecting helicobacter pylori in an oral cavity sample.

The invention provides a method for simply detecting lead content in a working environment. The method comprises the following steps: absorbing lead-containing particles in a certain volume of air on a working site for sampling; taking out an absorber, soaking the absorber in an accommodating bottle for accommodating a mixed solution of sulfuric acid and sodium chloride, and washing the lead-containing particles completely; using ultrasonic waves to smash and disperse the lead-containing particles in the accommodating bottle; filtering impurities; dropping the quantitative lead ion solution in a methyl thymol blue chelate resin solid detection agent; measuring the length of a color-changing strip, contrasting with a standard curve to obtain lead ion content in the lead ion solution, and then calculating to obtain the lead content in the certain volume of air on the working site. Therefore, the whole process of sampling and detection can be realized smoothly and quickly, and accurate and efficient lead content detection on the working site can be achieved. The invention further provides a sampling device with high portability and a detachable combination function, the sealing of a sampling absorption working area can be effectively realized, and the working effectiveness and sampling index stability are guaranteed.

The invention relates to a method for determining the effective substance content of petroleum sulfonate, which comprises the following steps: weighing 0.5-1.0g of petroleum sulfonate; putting the petroleum sulfonate in a 25mL measuring flask, shaking up, and diluting to the specified volume, thus obtaining a detection sample; putting 1mL of detection sample in a 100mL measuring cylinder with a stopper, adding 5-8 drops of mixed indicator, 5-10 drops of thymol blue indicator and 5-10mL of sodium sulfate acidic solution, adding water to keep the water phase at 30-35mL, adding 15-20mL of dichloromethane, oscillating, and shaking up to obtain a mixed sample, wherein the mixed indicator is a mixture of methylene blue and methyl orange; titrating the mixed sample with a CTMAB (cetyl trimethyl ammonium bromide) solution until the titration end point (namely the bottom phase is changed from blue to green); and calculating the concentration of the sulfonate according to the titration result. The method has the advantages of high precision, simple and quick operation process, high reliability, clear end point and less interference.

The invention relates to a thermosensitive time indicating device. The thermosensitive time indicating device comprises a base layer, a first substrate layer which forms an accommodation body with the base layer, a volatile functional layer which is accommodated in the accommodation body and comprises a phase change material selected from at least one of oleic acid, elaidic acid, n-nonanoic acid, octanoic acid, glyceryl triacetate and lauric acid, an adsorption indicating layer which is formed on the surface of the first substrate layer and contains an indicator composition, and a second substrate layer which is formed on the surface of the adsorption indicating layer and has the advantages of transparency and airtightness. The indicator composition comprises at least one of a pH sensitive dye. The pH sensitive dye is selected from at least one of m-cresol violet, anthocyanins, thymolphidin, o-cresolphthalein, bromothymol blue, cresol red, cetyltrimethylammonium cation, neutral red, phenol red, rhodamine, sulfo rhodamine 101 and thymol blue. The thermosensitive time indicating device is simple in structure, and is advantageous for miniaturization.

The invention discloses a method for determining the lead content in rapeseed oil. The method for determining the lead content in the rapeseed oil comprises the following steps that 1, a 10-phenanthroline solution, a hydroxylammonium chloride solution and a 0.1% thymol blue indicator are prepared; 2, a lead standard solution is prepared; 3, the double-lead content is regarded as an x-coordinate and the absorbance is regarded as a y-coordinate to make a lead standard curve; 4, the rapeseed oil is weighed, concentrated hydrochloric acid, water and glass beads are added, reflux boiling is performed, lower-layer acid extract liquid is filtered by double-layer filter paper after standing and layering, filtrate is fed into a beaker, a conical flask is cleaned, the liquid is poured into a funnel and then is mixed with the filtrate in the beaker after layering, the filter paper is flushed, drying by distillation is performed, and water is added for constant volume so as to obtain a liquid sample; 5, the liquid sample obtained in the step 4 is absorbed in a colorimetric tube, and a lead marking curve is made as the step 3. The method for determining the lead content in the rapeseed oil is quick, accurate, simple and convenient to operate, high in sensitivity and smaller in environment pollution, does not use a highly toxic agent, has a minor hazard to human health and can meet the testing demands of ordinary experimenters.

The invention relates to stability augmentation test paper for determining ammonia in air by a drying method and preparation and detection methods thereof, and belongs to the fields of environmental monitoring technology and industrial hygiene. The stability augmentation test paper for determining ammonia in the air by the drying method is the test paper loaded with a bromocresol purple-bromothymol blue mixed indicator and a cationic surfactant on base paper, which has a mass ratio of bromocresol purple: bromothymol blue: cationic surfactant = (0.1-0.55): (0.025-0.2): (X-X'). An X-X' value varies depending on the surfactant. The preparation method comprises the following steps of: preparing a soaking liquid according to a reagent ratio; soaking the base paper; and drying and carrying out plastic packaging. The detection method comprises the following steps of: detecting the reaction of ammonia in the air and a mixed reagent of the test paper; turning from yellow to bluish violet; and obtaining the concentration of the ammonia in the air of a detection area according to the relationship between the length of a color changing belt of the test paper and the ammonia concentration. Thedetection method can be used to measure the ammonia concentration in the air of a workshop or a production site or the emergency monitoring of the ammonia leakage.

The invention discloses a vibrio medium for detecting the content of vibrio in aquaculture water. Specifically, the vibrio medium comprises 2.0g of glutamic acid, 15.0g of tryptone, 15.0g of sodium thiosulfate, 5.0g of glycerol monolaurate, 20.0g of lactose, 15.0g of sodium chloride, 1.5g of ammonium ferric citrate, 0.04g of thymol blue, 20.0g of agar powder, and 1000ml of distilled water, and has a pH value of 7.5. The components are mixed, and heated to boiling for 35min, then the product is poured into 22ml/dish culture dish, and after solidification, the medium can be used for vibrio detection. The vibrio medium for detecting the content of vibrio in aquaculture water provided by the invention has high vibrio detection rate, can accurately reflect the total amount of vibrios in culture water, has a vibrio detection rate significantly superior to that of a TCBS medium, avoids the phenomenon of serious discrepancy between vibrio amount detection by a TCBS medium and an actual value during culture, and has important technological value for prevention of aquaculture vibriosis.