8 results about "BROMTHYMOL BLUE" patented technology

The present invention provides a visual indicator film for the freshness of fresh-cut fruits, a preparation method, the use and a detection method. The preparation method comprises the following steps: preparing a Co-based MOF; washing and activating the Co-MOF; preparing a CMC-Na-based thin film; and preparing a CMC-Na-based thin film, and thereby obtaining an indicator film for phenol red (PR) or an indicator film for bromothymol blue (BTB). In the present invention, an irregular spherical Co-based metal organic framework (Co-MOF) loaded with PR or BTB is prepared, and the nanocomposites Co-MOF / PR and Co-MOF / BTB are obtained after dyes and the MOF are adsorbed by means of the hydrogen bond interaction. Then, the nanocomposites are embedded into a sodium carboxymethyl cellulose (CMC-Na) base material to prepare CO2-responsive packaging materials, namely, a CMC-Na / PR / Co-MOF and a CMC-Na / BTB / Co-MOF, which are used for monitoring the freshness of fruits in real time and in situ. The Co-MOF-based composite thin film has good water resistance, time-temperature stability and mechanical properties, and also exhibits enhanced ultraviolet-visible light barrier properties, dye migration resistance and thermal stability. The visual indicator film for the freshness of fresh-cut fruits can effectively reflect the concentration of CO2, and is suitable for real-time and in-situ monitoring of the freshness of fruits.

The application discloses a chromogenic culture medium for detecting enteropathogenic Escherichia coli, which contains peptone, sodium pyruvate, sodium chloride, D-sorbitol, D-malic acid, bile salt, dipotassium hydrogen phosphate, potassium dihydrogen phosphate, sodium hydroxide, kaolin, bromothymol blue, crystal violet, polyethylene glycol and agar, and the rest is water. The chromogenic culture medium is simple in composition, convenient to prepare and use, and can be used for identifying enteropathogenic Escherichia coli EPEC according to the color of bacterial colonies, and has the advantages of specificity, high efficiency, rapidness, low cost, simple result judgment and the like, and has a wide application prospect in food safety monitoring.

The invention discloses a detection method of inflammatory marker protein. According to the method, the micro-fluidic chip with a sample loading area, a buffer treatment area, a sample channel and a detection area is prepared on filter paper through wax printing; chitosan is preset in the sample loading area and is used for treating a sample, and bromothymol blue is fixed in the detection area and is used as a pH indicator. During detection, a sample is driven by a phosphate buffer solution with the pH value of 6.0 to flow to a detection area through capillary action, the C-reactive protein and the indicator are subjected to non-specific binding, so that the color of the detection area is changed from yellow to green, the length of a green developing signal is in direct proportion to the concentration of the CRP, and quantitative detection can be realized by measuring the length. The method does not need an antibody, is low in cost, is simple and convenient to operate, can complete detection within 15 minutes, has the detection limit of 1.56 ng / ml and the linear range of 5-25 ng / ml, and is suitable for rapid screening and field detection of basic medical institutions.

The application provides a preparation method of a water-resistant intelligent colorimetric label for indicating food freshness, and belongs to the technical field of food packaging. A mixed dye is prepared by taking methyl red and bromothymol blue as raw materials, then the aqueous phase solution is obtained by adding the mixed dye into a chitosan gelatin solution, and the aqueous phase solution is added into an oil phase containing an emulsifier to perform emulsification and crosslinking, so that pH color developing microspheres are formed, and finally, the pouring method is adopted to prepare the water-resistant intelligent colorimetric label for indicating food freshness. The colorimetric label prepared by the application embeds the methyl red and the bromothymol blue in the ethyl cellulose film by using the microsphere method, which not only ensures the response of the colorimetric label to the acidic gas, but also avoids the influence of the environmental humidity on the indication accuracy, and the methyl red and the bromothymol blue cannot be dissolved out under high humidity, so that the stability of the film in the high humidity environment is ensured.

The invention discloses a double-layer hydrogel dressing for chronic wound diagnosis and treatment and a preparation method thereof. The dressing is formed by compounding a pH response monitoring layer and an anti-inflammatory and antibacterial treatment layer. The monitoring layer is loaded with bromothymol blue, visual color response can be generated according to the pH change of the wound microenvironment, and real-time monitoring of infection is achieved; the treatment layer takes a methacrylated gelatin / sodium alginate dual-network as a base material, is loaded with quercetin liposome and copper ions, and has long-acting anti-inflammatory and efficient antibacterial functions. The mechanical performance of the hydrogel is enhanced by constructing a double-network structure, and in-vitro experiments prove that the hydrogel has excellent pH visual response performance and broad-spectrum killing capacity on various pathogenic bacteria. The real-time diagnosis and treatment functions are integrated, and the method has important application prospects in the aspects of accurate management and healing promotion of diabetic chronic wounds.

This invention discloses a method for preparing and applying an antibacterial hydrogel biomimetic skin. The antibacterial hydrogel biomimetic skin comprises an N-isopropylacrylamide and [2-(methacryloyloxy)ethyl]dimethyl-(3-sulfopropyl)ammonium hydroxide hydrogel matrix, and a photothermal filler and bromothymol blue indicator loaded within the hydrogel matrix. The photothermal filler is assembled from silver nanoparticles with antibacterial properties and two-dimensional transition metal carbon / nitrides. This antibacterial hydrogel biomimetic skin resembles human skin, possessing multiple resistance mechanisms against bacteria: antifouling, detection, inactivation, and self-cleaning. The hydrogel matrix of this antibacterial hydrogel biomimetic skin is temperature-sensitive, with a controllable peak temperature, achieving intelligent antibacterial protection when combined with photothermal sterilization technology; the inactivation rate against *Escherichia coli* and *Staphylococcus aureus* is 100%. The preparation method of this invention is simple, low-cost, and requires minimal equipment investment, making it suitable for medical and health applications, especially for the protection of medical devices and biomaterials.

This invention relates to the field of polymer materials technology, and in particular to an aromatic dissolution-separation type color-changing microneedle patch, its preparation, and its application in the preparation of products for treating diabetic foot ulcers. The microneedle patch comprises a needle layer and a substrate layer placed beneath the needle layer; the needle layer is formed by compounding self-assembled carrier-free nanoparticles containing thymol and an aqueous solution of hyaluronic acid; the substrate layer is formed by compounding an aqueous solution of bromothymol blue and polyvinyl alcohol. The aromatic dissolution-separation type color-changing microneedle patch prepared by this invention possesses the ability to rapidly absorb moisture and self-dissolve after penetrating the skin, without remaining in the body, thus improving biocompatibility and promoting transdermal absorption of active substances, thereby achieving a slow and controllable release of fragrance from within the skin tissue; this microneedle patch has triple effects of antibacterial, anti-inflammatory, and antioxidant properties, opening a new path for the clinical application of diabetic foot ulcers.

This invention relates to a charge transfer complex hydrogel, its preparation method, and its application. The charge transfer complex hydrogel comprises: a solution of charge transfer complex nanoparticles and a bromothymol blue solution, wherein the charge transfer complex nanoparticles are 3-AP-TQ NPs, Per-TQ NPs, 3-BrP-TQ NPs, or 3-PCA-TQ NPs. The charge transfer complex hydrogel of this invention possesses the following functions: highly efficient photothermal antibacterial properties: utilizing the photothermal effect of NIR-II CTCs, localized high temperatures are generated under laser irradiation to kill drug-resistant bacteria; real-time infection monitoring: the degree of infection is visually indicated by the sensitive color change of BTB on wound pH; ideal dressing properties: relying on a carrageenan base, it provides a suitable moist environment, permeability, and mechanical support.