17 results about "BROMTHYMOL BLUE" patented technology

The invention relates to the technical field of biomedical materials, in particular to a microneedle patch for diabetes wound healing and a preparation method and application thereof. The microneedle patch comprises a substrate and a microneedle array arranged on the surface of the substrate, the substrate is prepared from a first raw material system comprising polyvinyl alcohol, phenylboronic acid modified oxidized hyaluronic acid, bromothymol blue and first drug micromolecules; the microneedle array is prepared from a second raw material system which comprises methylacryloylated gelatin, 2-(methylacryloyloxy) ethyl choline phosphate and platelet cell membrane wrapped MOF loaded second drug micromolecules. The microneedle patch has the characteristics of promoting angiogenesis, being excellent in antibacterial property and slow release, also has sensitive pH responsiveness and ROS responsiveness at the same time, can adjust the release dose of the medicine according to the wound surface microenvironment change (pH value change and ROS concentration change), can monitor the wound surface state in real time according to the wound surface pH value change indication, and is suitable for being popularized and used clinically.

The present invention belongs to the technical field of rapid detection of drug sensitivity for bacteria. Disclosed are an enrichment culture medium, and a preparation method therefor and the use thereof. The enrichment culture medium comprises the following raw materials in parts by mass: 15.0-20.0 parts of tryptone, 3.5-5.5 parts of peptone, 1.5-3.5 parts of glucose, 5.0-6.5 parts of yeast extract, 2.0-2.5 parts of dipotassium phosphate, 2.0-3.5 parts of disodium hydrogen phosphate, 1.0-1.5 parts of monopotassium phosphate, 2.5-7.5 parts of sodium chloride, 12.0-18.0 parts of proteose peptone, 15.0-22.0 parts of beef heart infusion powder, 8.0-12.0 parts of soya peptone, 1.0-5.0 parts of 0.1% nicotinamide adenine dinucleotide (NAD), 30-100 parts of newborn calf serum inactivated at 56°C for 30 min, 2.0-4.0 parts of mannitol, 3.0-5.0 parts of sodium pyruvate, 0.23-0.45 parts of cycloheximide, 0.8-2.0 parts of 0.05% bromothymol blue, 2.5-4.5 parts of 0.1% methylene blue, and 1000 parts of distilled water, with the pH value of 6.8-7.5. The enrichment culture medium is used to screen effective therapeutic drugs for bacterial diseases in poultry, and is convenient to use and has a single enrichment effect superior to that of respective selective enrichment broths of target bacteria. Therefore, the culture medium can realize rapid proliferation of common pathogenic bacteria in poultry.

The present invention provides a visual indicator film for the freshness of fresh-cut fruits, a preparation method, the use and a detection method. The preparation method comprises the following steps: preparing a Co-based MOF; washing and activating the Co-MOF; preparing a CMC-Na-based thin film; and preparing a CMC-Na-based thin film, and thereby obtaining an indicator film for phenol red (PR) or an indicator film for bromothymol blue (BTB). In the present invention, an irregular spherical Co-based metal organic framework (Co-MOF) loaded with PR or BTB is prepared, and the nanocomposites Co-MOF / PR and Co-MOF / BTB are obtained after dyes and the MOF are adsorbed by means of the hydrogen bond interaction. Then, the nanocomposites are embedded into a sodium carboxymethyl cellulose (CMC-Na) base material to prepare CO2-responsive packaging materials, namely, a CMC-Na / PR / Co-MOF and a CMC-Na / BTB / Co-MOF, which are used for monitoring the freshness of fruits in real time and in situ. The Co-MOF-based composite thin film has good water resistance, time-temperature stability and mechanical properties, and also exhibits enhanced ultraviolet-visible light barrier properties, dye migration resistance and thermal stability. The visual indicator film for the freshness of fresh-cut fruits can effectively reflect the concentration of CO2, and is suitable for real-time and in-situ monitoring of the freshness of fruits.

The application discloses a chromogenic culture medium for detecting enteropathogenic Escherichia coli, which contains peptone, sodium pyruvate, sodium chloride, D-sorbitol, D-malic acid, bile salt, dipotassium hydrogen phosphate, potassium dihydrogen phosphate, sodium hydroxide, kaolin, bromothymol blue, crystal violet, polyethylene glycol and agar, and the rest is water. The chromogenic culture medium is simple in composition, convenient to prepare and use, and can be used for identifying enteropathogenic Escherichia coli EPEC according to the color of bacterial colonies, and has the advantages of specificity, high efficiency, rapidness, low cost, simple result judgment and the like, and has a wide application prospect in food safety monitoring.

The invention discloses a detection method of inflammatory marker protein. According to the method, the micro-fluidic chip with a sample loading area, a buffer treatment area, a sample channel and a detection area is prepared on filter paper through wax printing; chitosan is preset in the sample loading area and is used for treating a sample, and bromothymol blue is fixed in the detection area and is used as a pH indicator. During detection, a sample is driven by a phosphate buffer solution with the pH value of 6.0 to flow to a detection area through capillary action, the C-reactive protein and the indicator are subjected to non-specific binding, so that the color of the detection area is changed from yellow to green, the length of a green developing signal is in direct proportion to the concentration of the CRP, and quantitative detection can be realized by measuring the length. The method does not need an antibody, is low in cost, is simple and convenient to operate, can complete detection within 15 minutes, has the detection limit of 1.56 ng / ml and the linear range of 5-25 ng / ml, and is suitable for rapid screening and field detection of basic medical institutions.

The application provides a preparation method of a water-resistant intelligent colorimetric label for indicating food freshness, and belongs to the technical field of food packaging. A mixed dye is prepared by taking methyl red and bromothymol blue as raw materials, then the aqueous phase solution is obtained by adding the mixed dye into a chitosan gelatin solution, and the aqueous phase solution is added into an oil phase containing an emulsifier to perform emulsification and crosslinking, so that pH color developing microspheres are formed, and finally, the pouring method is adopted to prepare the water-resistant intelligent colorimetric label for indicating food freshness. The colorimetric label prepared by the application embeds the methyl red and the bromothymol blue in the ethyl cellulose film by using the microsphere method, which not only ensures the response of the colorimetric label to the acidic gas, but also avoids the influence of the environmental humidity on the indication accuracy, and the methyl red and the bromothymol blue cannot be dissolved out under high humidity, so that the stability of the film in the high humidity environment is ensured.

This invention relates to a method for preparing dimensionally stable smart indicator labels using wood with retained lignin. It addresses the technical problems of existing blending methods for preparing smart indicator films, such as high cost and difficulty in identifying color changes due to humidity and transparency variations. The method involves: mixing epichlorohydrin, triethylamine, and ethanol to obtain a reaction solution; soaking poplar veneer in an alkaline solution to swell it before adding it to the reaction solution to obtain quaternized poplar veneer; then immersing the quaternized poplar veneer in a dye solution to adsorb bromothymol blue, followed by soaking in deionized water until neutral, and freeze-drying to obtain the smart indicator label. This label is highly sensitive to acetic acid gas, exhibiting a clear color change. When indicating milk freshness, the label changes from blue to green when °T is near the critical value specified by the national standard, providing a quick and accurate indication of milk freshness. This invention's smart indicator label can be used in the field of food testing.

The invention provides a mixture containing microcapsules, a preparation method of the mixture and a nursing pad, and belongs to the technical field of medical monitoring nursing materials. Methyl red and bromothymol blue in the microcapsule-containing mixture provided by the invention are acid-base indicators, so that the nursing pad has a pH value indication effect; the persimmon extract has antibacterial and deodorizing effects; the polyhexamethylene guanidine has a broad-spectrum antibacterial effect; the polyethylene glycol has good solubility, lubricity, moisture retention and the like, so that the solubility, the stability and the bioavailability of the raw materials are improved; part of substances (methyl red, bromothymol blue, persimmon extract, polyhexamethylene guanidine and polyethylene glycol) in the raw materials can be contained in hydrophobic cavities of cyclodextrine to form a microcapsule structure, so that the stability and dispersity of the raw materials are improved, slow and continuous release is realized, the nursing pad has long-time antibacterial and deodorant effects, and the service life of the nursing pad is prolonged. And the color developing uniformity of the nursing pad can be improved.

The invention provides a helicobacter pylori allochroic isolation medium as well as a preparation method and application thereof, and belongs to the technical field of helicobacter pylori culture. The helicobacter pylori color-changing separation culture medium is prepared from the following components in concentration: 4 to 6 g / L of bovine brain extract powder, 4 to 6 g / L of bovine heart extract powder, 10 to 15 g / L of agar, 21 to 25 g / L of casein peptone, 2.5 to 3.5 g / L of glucose, 4 to 6 g / L of sodium chloride, 2.5 to 3.5 g / L of disodium hydrogen phosphate, 0.5 to 1.5 g / L of beta-cyclodextrin, 1.5 to 2.5 g / L of urea, 0.1 to 0.3 g / L of bromothymol blue, 0.01 to 0.05 mL / L of bacteriostatic agent, 80 to 100 mL / L of calf serum and a solvent of water. The culture medium can obviously improve the separation culture success rate of the helicobacter pylori clinical strain, and has the characteristics of high efficiency, specificity, high bacterial colony activity and color change.

The invention discloses hydrogel with pH responsiveness as well as a preparation method and application of the hydrogel. The preparation method comprises the following steps: stirring and dispersing the hyaluronic acid, the N, N-diisopropylethylamine, the 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide and the N-hydroxysuccinimide into water; gentamicin sulfate is added, and stirring is continued; performing freeze-drying; carrying out hydration to form hydrogel; soaking in a mesoporous silica suspension; and soaking in a bromothymol blue solution. According to the invention, hyaluronic acid, gentamicin sulfate, mesoporous silica and bromothymol blue are combined to prepare the hydrogel with pH responsiveness. The hydrogel can release gentamicin sulfate through hydrolysis to effectively resist bacteria, and the color of the loaded pH sensitive indicator bromothymol blue can also generate characteristic change along with the change of the pH value of the wound microenvironment, so that the visual monitoring of the wound state is realized.

The invention relates to the technical field of medical dressings, and particularly discloses a discoloring dressing capable of tracing retention time and a preparation method thereof, the discoloring dressing comprises a base material layer, a time indication layer, an oxygen sensitive composite film and a microbial response layer; the base material layer is formed by one of medical non-woven fabric and polyurethane foam; the time indication layer is arranged on the upper surface of the base material layer and comprises a pH response type color-changing material; the oxygen-sensitive composite film covers the surface of the time indicating layer and comprises platinum (II)-tetraphenyl porphyrin ketone (PtOEPK) dispersed in a polydimethylsiloxane (PDMS) matrix, and the color change of the oxygen-sensitive composite film is related to the tissue oxygen partial pressure; through the arrangement of the time indication layer, a compound of bromcresol purple and bromothymol blue is adopted as a pH response type color change material, so that the dressing can present gradient color changes of yellow, green and blue according to the pH change of a wound in the retention process, the retention time of the dressing is visually displayed, and medical staff can conveniently judge the replacement time.

The invention discloses a double-layer hydrogel dressing for chronic wound diagnosis and treatment and a preparation method thereof. The dressing is formed by compounding a pH response monitoring layer and an anti-inflammatory and antibacterial treatment layer. The monitoring layer is loaded with bromothymol blue, visual color response can be generated according to the pH change of the wound microenvironment, and real-time monitoring of infection is achieved; the treatment layer takes a methacrylated gelatin / sodium alginate dual-network as a base material, is loaded with quercetin liposome and copper ions, and has long-acting anti-inflammatory and efficient antibacterial functions. The mechanical performance of the hydrogel is enhanced by constructing a double-network structure, and in-vitro experiments prove that the hydrogel has excellent pH visual response performance and broad-spectrum killing capacity on various pathogenic bacteria. The real-time diagnosis and treatment functions are integrated, and the method has important application prospects in the aspects of accurate management and healing promotion of diabetic chronic wounds.

The invention discloses a dressing material for naked eye visual wound infection monitoring and a preparation method thereof, and the preparation method comprises the following steps: adding thionyl chloride into a mixed system of carboxymethyl cellulose fiber and a solvent to obtain acylating chlorinated carboxymethyl cellulose fiber so as to activate carboxyl; and mixing the carboxymethyl cellulose fibers with pH-responsive color-changing dyes such as bromothymol blue or phenol red, and heating to react, so as to obtain the carboxymethyl cellulose fibers covalently grafted with the pH-responsive color-changing dyes. Carboxyl on carboxymethyl cellulose is subjected to acylating chlorination, the reaction activity of the site is improved, the site can chemically react with phenolic hydroxyl on the pH-responsive color-changing dye, the carboxymethyl cellulose fiber covalently grafted with the pH-responsive color-changing dye is obtained, the risk of dye exudation is greatly reduced, and excellent safety is achieved. The dressing material obtained by the invention has an excellent pH-responsive discoloration function, can generate pH-responsive discoloration in a key pH range (pH 6-8) of wound infection indication, achieves the purpose of wound infection indication, and has a wide actual clinical application prospect.

This invention discloses a method for preparing and applying an antibacterial hydrogel biomimetic skin. The antibacterial hydrogel biomimetic skin comprises an N-isopropylacrylamide and [2-(methacryloyloxy)ethyl]dimethyl-(3-sulfopropyl)ammonium hydroxide hydrogel matrix, and a photothermal filler and bromothymol blue indicator loaded within the hydrogel matrix. The photothermal filler is assembled from silver nanoparticles with antibacterial properties and two-dimensional transition metal carbon / nitrides. This antibacterial hydrogel biomimetic skin resembles human skin, possessing multiple resistance mechanisms against bacteria: antifouling, detection, inactivation, and self-cleaning. The hydrogel matrix of this antibacterial hydrogel biomimetic skin is temperature-sensitive, with a controllable peak temperature, achieving intelligent antibacterial protection when combined with photothermal sterilization technology; the inactivation rate against *Escherichia coli* and *Staphylococcus aureus* is 100%. The preparation method of this invention is simple, low-cost, and requires minimal equipment investment, making it suitable for medical and health applications, especially for the protection of medical devices and biomaterials.

This invention relates to the field of polymer materials technology, and in particular to an aromatic dissolution-separation type color-changing microneedle patch, its preparation, and its application in the preparation of products for treating diabetic foot ulcers. The microneedle patch comprises a needle layer and a substrate layer placed beneath the needle layer; the needle layer is formed by compounding self-assembled carrier-free nanoparticles containing thymol and an aqueous solution of hyaluronic acid; the substrate layer is formed by compounding an aqueous solution of bromothymol blue and polyvinyl alcohol. The aromatic dissolution-separation type color-changing microneedle patch prepared by this invention possesses the ability to rapidly absorb moisture and self-dissolve after penetrating the skin, without remaining in the body, thus improving biocompatibility and promoting transdermal absorption of active substances, thereby achieving a slow and controllable release of fragrance from within the skin tissue; this microneedle patch has triple effects of antibacterial, anti-inflammatory, and antioxidant properties, opening a new path for the clinical application of diabetic foot ulcers.

This invention relates to a charge transfer complex hydrogel, its preparation method, and its application. The charge transfer complex hydrogel comprises: a solution of charge transfer complex nanoparticles and a bromothymol blue solution, wherein the charge transfer complex nanoparticles are 3-AP-TQ NPs, Per-TQ NPs, 3-BrP-TQ NPs, or 3-PCA-TQ NPs. The charge transfer complex hydrogel of this invention possesses the following functions: highly efficient photothermal antibacterial properties: utilizing the photothermal effect of NIR-II CTCs, localized high temperatures are generated under laser irradiation to kill drug-resistant bacteria; real-time infection monitoring: the degree of infection is visually indicated by the sensitive color change of BTB on wound pH; ideal dressing properties: relying on a carrageenan base, it provides a suitable moist environment, permeability, and mechanical support.

The invention belongs to the technical field of detection, and particularly relates to a detection method of ammonium fluoride. The detection method comprises the following steps: S1, detecting the concentration of free acid in an ammonium fluoride product by adopting a titration method, S2, adding a formaldehyde aqueous solution into the product obtained after detection in the step S1, and S3, detecting the concentration of ammonium fluoride in the product in the step S2 by adopting a titration method. Compared with an existing detection method in which the concentrations of the free acid and the ammonium fluoride are respectively detected, the method has the advantages that two independent steps are combined into two continuous steps, the processes of reagent preparation and the like are reduced, the detection efficiency is improved, the method is more suitable for automatic detection, and the efficiency and the accuracy are further improved. During manual acid-base titration, bromothymol blue is used as an indicator, and compared with bromocresol purple and phenolphthalein which are used as titration indicators in the prior art, the titration end point judgment is accurate and is closer to neutral, so that the obtained concentrations of free acid and ammonium fluoride are more accurate compared with the prior art.