56 results about "BROMTHYMOL BLUE" patented technology

The invention relates to the field of food detection, and relates to a preparation method of intelligent indicator labels for freshness of meat, and application, and the preparation method comprises the following steps of firstly preparing a fluorescent carbon quantum dot, dissolving the fluorescent carbon quantum dot in ethanol with bromothymol blue, and adjusting the pH value to obtain a freshness indicator; mixing the chitosan solution, polyvinyl alcohol solution and freshness indicator, forming an indicator film after being de-foamed and dried by ultrasound, and wrapping with a low densitypolyethylene film to obtain intelligent freshness indicator labels; recording the visible light color pictures of the labels for different storage days after coexisting the labels with the meat to form a visible light colorimetric card, and recording the fluorescent color pictures to form a fluorescent color colorimetric card; and when the colors of the labels change after the meat to be tested and the labels coexist, realizing the visual detection of the freshness of the meat through the color comparison of the colorimetric cards. The labels prepared by the invention are safe and environmentally friendly, and the fluorescent nano-materials combined with the traditional color indicators make the color signals of the indicators more abundant and the measurement results more accurate.

The invention relates to the technical field of fish detection, and particularly relates to fish freshness detection test paper. Each liter of an indicator solution in a reagent layer contains 0.5-1g of bromophenol red, 0.6-1.1g of bromcresol purple, 0.3-0.5g of bromothymol blue, 0.3-0.8g of neutral red, 0.2-0.6g of phenol red and the balance of a solvent. The fish freshness is detected rapidly with low cost and high flexibility. The detection test paper can be used for detecting the freshness of chilled, refrigerated and frozen fish. The using method is simple; and only less amount of fish body surface mucus is dipped when the test paper is used, and the fish freshness can be obtained by comparing the color instantly developed by the test paper with a standard color chart.

A sensor for detecting a presence of bacteria in a perishable food includes a pH sensitive solution of bromothymol blue and methyl red mixed with an alkaline resulting in a pH value and a generally green color changing to a generally orange color responsive to exposure to a concentration of carbon dioxide. The solution is packaged in a gas permeable container using a TPX (PMP) thin film that allows an effective diffusion of carbon dioxide through the container. The pH level drops when acidic carbon dioxide comes into contact with the solution resulting from a formation of carbonic acid, making the solution an indicator of carbon dioxide concentration, and thus an indication of bacterial growth.

The invention relates to a rice metabolism detecting reagent and method, concretely used in the condition of detecting rice metabolism. It mainly takes and dissolves nitrazine yellow / chlorophenol red, p-nitrophenol, bromocresol green / bromothymol blue / resorcinol blue, bromocresol purple, and hematoxylin / cresol red / bromophenol red / methyl red into alcohol, and mixing them uniformly to obtain finished product of raw reagent liquid. As detecting, taking a raw reagent liquid and adding distilled water to dilute the raw reagent liquid so as to form a liquid reagent, adding rice to the liquid reagent to react, observing the colors of rice and solution, judging the metalolism degree of the rice detected, the metabolism situation and color of the rice corresponds to green and passes through yellow and salmon pink into red region, where the green expresses new rice, yellow expresses old rice and salmon pink expresses deeply aged rice. The reagent of the invention is lower-cost and has simple manufacturing process; the operation is quick, accurate and convenient; the detecting method is simple, able to be used in both qualitative detection and quantitative detection.

The invention discloses a legionnella selective separation medium. Dyes such as bromothymol blue and bromocresol purple are added to the basic formulation of the basic medium of legionnella BCYE alphaand legionnella selective antibiotics of glycin, vancocin, polymyxin B and actinone are also added. The added dyes produce pigment in the growth process of legionnella; the shape and the color of a colony are beneficial to identifying and distinguishing a target bacterium of the legionnella; the concentrations of the selective antibiotics are added and regulated, the inhibiting capability to non-legionnella and microbial class is enhanced and the inhibiting performance for the legionnella is reduced, thereby enhancing the positive rate for the separation culture of the legionnella; the detection cost is reduced and the legionnella selective separation medium is economic and practical and is convenient for popularization and application for clinical and health and epidemic prevention departments.

The invention discloses an amniotic fluid detection indicator composition, preparation thereof and a corresponding amniotic fluid detection pad. The amniotic fluid detection indicator composition is used by taking one or two of water, ethyl alcohol, acetone and N,N-dimethylformamide as a solvent or solvents, taking one or more than one of pulullan, sodium alginate, ethyl cellulose and sodium carboxymethylcellulose as a film-forming agent or film-forming agents, taking dioctyl phthalate as a plasticizer, taking ethylene glycol, glycerol and glycol ethylene ether as wetting agents, taking bromothymol blue sodium salt, bromoxylenol blue, sodium alizarine sulfonate and bromcresol purple as indicators, preparing an amniotic fluid detection indicator at the normal temperature, then coating a medical sanitary pad with the amniotic fluid detection indicator, and drying the medical sanitary pad to prepare an amniotic fluid detection pad. The amniotic fluid detection indicator composition is convenient to use, not easy to decolor and more easily acceptable to patients, and is capable of distinguishing amniotic fluid and urine; the whole process is simple to operate; the large-scale production is easy to implement.

Provided is a CO2 sensitive type indicator label manufacturing method. An ethanol solution of 10%-90% is prepared; methyl red of 0.07-0.12 g dissolves in the ethanol solution of 100 mL, and a methyl red ethanol solution is obtained; bromothymol blue of 0.08-0.15 g dissolves in the ethanol solution of 100 mL, and a bromothymol blue ethanol solution is obtained; the methyl red ethanol solution of 10-55 mL and the bromothymol blue ethanol solution of 15-50 mL are mixed and are diluted tenfold, and an indicator solution is obtained; glycerinum is added and is heated in a water bath at constant temperature, methylcellulose of 1.2-2.2 g is thrown in when the glycerinum reaches the rated temperature, and the mixture is continuously stirred at a high speed to form an even methylcellulose solution; as the temperature decreases, the viscosity of the solution is increased; indicator membrane liquid is obtained; the indicator membrane liquid is scratched off on cotton fiber paper, air blasting and drying are carried out, and a CO2 sensitive type indicator label is obtained. The CO2 sensitive type indicator label is applicable to rapid detection with 0-100% CO2 content in environment.

The invention relates to cat litter and a preparing method thereof. Bean dregs, corn flour, corn starch, flour, extruded corn flour, calcium carbonate, plant fibers, emulsified equilibrium oil powder,guar gum, sodium polyacrylate and the like are taken as raw materials of the cat litter, then bromothymol blue is added into the raw materials, and the pH of urine of a kitty can be monitored at anytime. Since the cat litter is reasonable in raw material and proportioning, monitoring is conducted timely and accurately, and the probability that the pet suffers from lithiases can be effectively reduced.

The invention provides an identification method for ginkgo seedling male and female plants, and the method is mainly directed at identification of the seedling stages of ginkgo seedling male and female plants in urban garden greening. By preparing a phosphate buffer solution, utilizing the chromogenic reaction of a bromothymol blue solution, and a proper treatment during a seedling stage, various conditions needed by early identification of ginkgo seedling male and female plants can be guaranteed. Comprising the steps of grinding, extraction, centrifugation and the like on the ginkgo seedling male and female plants, the provided method can be applied in large areas during production practice of urban garden greening, and can solve the identification problem of ginkgo seedling male and female plants. Being effective, the method has the advantages of simple operation, high accuracy, reduction of identification time, and cost saving.

The invention provides a reagent which is capable of sensitively indicating the grain fatty acid value change only by means of visual colorimetric analysis and a detection method of the reagent, and the grain aging degree is indicated by the grain fatty acid value change. The detection reagent is prepared by the components, by weight, of 0.01%-0.1% of neutral red, 0.01%-0.1% of Bromothymol blue, 0.005%-0.05% of sodium carbonate, 10%-50% by volume of propylene glycol and the balance of water; the detection method comprises the steps that (1) appropriate components are weighed and dissolved, and the detection reagent is prepared; (2) a to-be-detected grain sample is placed on a piece of flat paper, the detection reagent is aimed at the grain sample and sprayed to the grain sample by one time, and the color change of the grain sample is observed within 1-10 minutes. According to the grain fatty acid value detection reagent, the detection method and application of the detection reagent, the manner of spraying the reagent to the surface of the grain sample is adopted, and the grain fatty acid value and the aging degree are detected by comparing the grain surface color change with a standard color card. Therefore, the method is easier to operate, the detection is more convenient, no other auxiliary equipment is required, and the operation time is saved.

The modified dermatophyte test culture medium is compounded with soybean peptone 5g / L, glucose 5 g / L, chloromycetin 0.0625-0.25g / L, cycloheximide 0.1-0.5g / L, bromthymol blue 0.025-0.1g / L, agar 20g / L, and 1N HCl 4.8mL, and has pH value of 5.5 + / - 0.1. The present invention has the advantages of capacity of resulting in accurate interpretation, short culture period and low cost, except the advantages of traditional DTM culture medium. The present invention has latent clinical application foreground.

The invention provides a vibrio quantity detecting kit comprising a vibrio diluent and a solid culture medium, wherein the vibrio diluent is a 0.009g / ml sodium chloride solution; and the solid culture medium comprises 3-5g of yeast cream, 6-10g of peptone, 12-20g of sucrose, 6-10g of sodium thiosulfate, 6-10g of sodium citrate, 1.8-3g of glycocholate sodium, 3-5g of ox gallbladder powder, 6-10g of sodium chloride, 0.6-1g of ferric citrate, 12-20ml of 2% bromothymol blue solution, 2.4-4ml of 1% thymol blue solution and 9-15g of agar according to a formula. The invention also provides a preparation method of the vibrio quantity detecting kit and a vibrio quantity detecting method realized by using the vibrio quantity detecting kit. The vibrio quantity detecting kit, the preparation method thereof and the vibrio quantity detecting method provided by the invention have the following technical effects of simplicity in operation, shorter detecting time and capability of effectively preventing and controlling vibriosis and providing a certain basis and guidance for clinical medication.

The invention relates to a needle mushroom strain detection culture medium and a usage method thereof, and belongs to the technical field of edible fungi strain degeneration detection. The invention aims at solving the technical problem of providing a needle mushroom strain activity degradation detection method which has the advantages of short detection period and high accuracy and a detection culture medium formula. The technical scheme is as follows: the needle mushroom strain detection culture medium comprises the following raw materials in parts by weight: 100-250 parts of potatoes, 10-30 parts of lactose, 1-3 parts of NH4NO3, 1-3 parts of KH2PO4, 0.1-1.5 parts of MgSO4.7H2O and 0.01-0.5 part of bromothymol blue. The needle mushroom strain detection culture medium disclosed by the invention can be used for culturing the strains to be detected; and according to the invention, the strains with good activity can decolorize the culture medium, and degraded strains or inactivated strains enable the color of the culture medium to be shallow or unchanged.

The invention discloses a vibrio test piece, which comprises a test piece body, a vibrio culture medium and a hydrogel carrier. The vibrio culture medium is prepared from the following components in percentage by mass: 2.2 to 3.5 percent of peptone, 1.5 to 2.0 percent of saccharose, 0.2 to 0.4 percent of ox gallbladder powder, 0.08 to 0.15 percent of sodium thiosulfate, 0.05 to 0.12 percent of ferric citrate, 0.002 to 0.005 percent of thymol blue, 1.0 to 1.5 percent of yeast, 0.8 to 1.0 percent of sodium chloride, 0.12 to 0.2 percent of deoxysodium cholate, 0.5 to 1.0 percent of trisodium citrate, 0.002 to 0.004 percent of bromothymol blue, 0.1 to 0.3 percent of xanthan gum, and the balance of pure water, wherein the pH value of the vibrio culture medium is 9.0 to 9.3. The invention also discloses a preparation method of the vibrio test piece. The vibrio test piece provided by the invention is convenient for a water body sample to absorb and diffuse in the culture medium, and providesa favorable growth environment for vibrio.

The invention provides a CO2 sensitive indicator and a preparation method thereof. The CO2 sensitive indicator comprises the following ingredients by mass percent: 0.001 to 0.021 percent of methyl red, 0.002 to 0.025 percent of bromothymol blue, 3 to 9 percent of ethanol and the balance being pure water with pH value of 7. The preparation method comprises the following steps: mixing ethanol and water to form a 50% to 85% ethanol solution a and a 5% to 60% ethanol solution b at room temperature; adding methyl red into the ethanol solution a, mixing and uniformly stirring to obtain a methyl red ethanol solution, adding bromothymol blue into the ethanol solution b, and mixing and uniformly stirring to obtain a bromothymol blue ethanol solution; and adding the methyl red ethanol solution and the bromothymol blue ethanol solution into the pure water with pH value of 7 to obtain the CO2 sensitive indicator. The indicator is suitable for repaid detection on CO2 with the content of 0 to 100 percent in an environment.

The present invention relates to a daily test paper, comprising a coloration bar for detection and a standard color plate, wherein the coloration bar uses pH base paper, the pH base paper is soaked in a reagent solution and then dried at constant temperature by hot wind. The reagent solution contains an indicator. The formula of the indicator comprises: aqueous coloring stabilizer 100 grams, cotton wood fiber anti-fungus agent 8 grams, anti-fungus agent for special paper 3 grams and buffer neutralizer prepared by sodium hydroxide 5 grams and phosphoric acid 12.5 grams, and the above components are dissolved in 30% aqueous ethanol solution 100 kg. The reagent solution also contains a sensitive reagent and the formula of the sensitive reagent comprises: ethyl red 20 grams, bromocresol green 50 grams, and bromthymol blue 200 grams. According to the preparation method provided by the invention, the pH value of the reagent solution is adjusted to 5.5-8, and the pH base paper is uniformly soaked and then dried at constant temperature by hot wind. The daily test paper effectively and clearly tests the weak acid, weak base and neutral pH value areas. The daily test paper can be used for relatively idea, convenient, practical and accurate tests.

The invention discloses a test paper slip for testing adulterated milk rapidly. The test paper slip is characterized in that a starch test paper slip for composing the test paper slip is produced by the steps of taking 10 ml of an iodine-potassium iodide solution with an I2 concentration of 0.032 mol / mL and a KI concentration of 0.126 mol / mL, diluting it with equal amount of a solvent, immersing completely quantitative test paper into the diluted iodine-potassium iodide solution for 5 minutes, airing the test paper obtained from the previous step at a temperature of 5 to 6 DEG C in darkness, clipping, and preserving it in a dark place; a carbamide test paper slip for composing the test paper slip is produced by the steps of taking 6 ml of a sodium nitrite hydrochloric acid solution with a sodium nitrite content of 0.04%, adding 0.4 g of a Griess reagent into the sodium nitrite hydrochloric acid solution, mixing well, immersing completely quantitative test paper into the mixture for 10 minutes, taking out the immersed test paper, airing it in the shade, clipping, and preserving it in a dark place; an edible alkali test paper slip for composing the test paper slip is produced by the steps of taking 10 ml of a prepared Bromothymol Blue ethanol indicator with a Bromothymol Blue content of 0.04%, placing the indicator on a disk, immersing completely quantitative test paper into the indicator for 10 to 15 minutes, taking out it, airing it, clipping, and preserving it in a dry place; and a salt test paper slip for composing the test paper slip is produced by the steps of mixing well 15 ml of a silver nitrate solution with a silver nitrate concentration of 0.01 mol / mL and six drops of a potassium chromate solution with a potassium chromate content of 10% quickly, immersing completely quantitative test paper into the mixture for about 20 minutes, taking out it, airing the immersed test paper, clipping, and preserving it in a dry place. The test paper slip provided by the invention can carry out an on-site test on raw milk, has a high sensitivity of test and can produce an accurate test result.

A test system for the determination of the presence of an antibiotic in a fluid using Bromthymol Blue as an indicator.

The invention belongs to the technical field of agricultural analysis, specifically relates to a special ammonia indicator for analyzing ammonia content by flow injection instruments. An import flow injection instrument is used for analyzing the ammonia contents of samples such as animal and plant life, food, water and soil, which has been normally-used instrument device and technology in domestic universities and colleges as well as scientific research, animal and plant life and food detection and check units. However, the most important reagent that is ammonia indicator for analyzing ammonia content was monopolized by foreign companies all the time, and the cost is expensive. The invention provides the proper mass ratio of prepared special ammonia indicators of flow injection instruments that are cresol red, bromcresol purple and bromothymol blue. The main technical targets of the special ammonia indicator in the invention all meet analysis requirements, and the cost of the reagent is 3.3% of that of contrast reagent.

The invention relates to a long-chain alkyltrimethyl ammonium sensitizing mixed reagent paper for measuring ammonia in air and preparation method thereof, belongs to the field of rapid environmental detection and industrial hygiene. The test paper is loaded with bromocresol purple sodium salt, bromothymol blue sodium salt, and surfactant long-chain alkyl trimethyl ammonium on the base paper according to the mass ratio, bromocresol purple sodium salt: bromo thymol blue sodium salt: surfactant long-chain alkyl trimethyl ammonium = (4~8): (6~10): (0.02~0.2). The preparation method comprises the following steps of: preparing the soaking solution according to the load reagent; soaking the base paper; drying and encapsulating. The test paper is made to change from the yellow to blue-violet basedon the reaction between ammonia gas in combination with bromocresol purple sodium salt and bromothymol blue sodium salt. The color change is obvious, the color of the test paper reaction is clear, itis easy to observe and measure, and the precision is high. The long-chain alkyltrimethyl ammonium makes the bromocresol purple sodium salt and the bromothymol blue sodium salt disperse better, and makes its uniformity on the filter paper better, thus the length of the test paper discoloration band is increased, and the sensitivity is increased.

The invention discloses a ceramic cleaning agent and a preparation method thereof. The ceramic cleaning agent is composed of industrial alcohol, glycol, a cation activator, an amphoteric surfactant, sodium ortho-cyclohexanediaminetetraacetate, citric acid, bromothymol blue, an aromatic and softened deionized water according to a certain weight percentage. The preparation method comprises the following steps of at a normal temperature, adding softened deionized water into a container, then adding industrial alcohol and glycol into the container, carrying out stirring for full mixing, adding other raw materials into the solution, carrying out full stirring until the raw materials are completely dissolved, and putting the solution into a pot, a bottle or a bag. The ceramic cleaning agent has good cleaning effects, can clean old and stubborn stains on the ceramic surface, is not influenced by a temperature easily, has no corrosivity, does not damage surface enamel and ceramic body of ceramic and realizes good ceramic surface brightness after cleaning. Cleaning waste water of the ceramic cleaning agent does not pollute the environment. The ceramic cleaning agent is a novel environmentally-friendly product and has effects of sterilization, disinfection and peculiar smell removal. The preparation method has simple processes and a moderate cost and can be popularized.

The invention relates to a thermosensitive time indicating device. The thermosensitive time indicating device comprises a base layer, a first substrate layer which forms an accommodation body with the base layer, a volatile functional layer which is accommodated in the accommodation body and comprises a phase change material selected from at least one of oleic acid, elaidic acid, n-nonanoic acid, octanoic acid, glyceryl triacetate and lauric acid, an adsorption indicating layer which is formed on the surface of the first substrate layer and contains an indicator composition, and a second substrate layer which is formed on the surface of the adsorption indicating layer and has the advantages of transparency and airtightness. The indicator composition comprises at least one of a pH sensitive dye. The pH sensitive dye is selected from at least one of m-cresol violet, anthocyanins, thymolphidin, o-cresolphthalein, bromothymol blue, cresol red, cetyltrimethylammonium cation, neutral red, phenol red, rhodamine, sulfo rhodamine 101 and thymol blue. The thermosensitive time indicating device is simple in structure, and is advantageous for miniaturization.

The invention belongs to the technical field of air tightness testing, and particularly relates to an air tightness high-precision testboard for a motor cover. One end of the testboard body is provided with a tester, and the other end is provided with a workbench; an airtight tool is arranged at the lower end of the workbench; fixing seats are arranged at the tail ends of piston rods of the cylinders; four blocking rods are fixed at the lower ends of the fixing seats; the detection module comprises a test paper board shell, a test paper board upper cover, wet bromothymol blue test paper and a blocking block; a blocking block is arranged at the lower end of the blocking rod and is detachable; the wet bromothymol blue test paper is positioned in the test paper plate shell; the test paper plate upper cover is positioned above the wet bromothymol blue test paper; the lower end of the test paper board shell is unclosed, the area of the unclosed lower end is smaller than that of the wet bromothymol blue test paper, and the test paper board shell and the test paper board upper cover are both made of transparent materials. According to the invention, the air leakage part of the test piece can be accurately tested, and the waste of resources is avoided.

The invention discloses high-performance mixed cat litter with functional color development health indication and deodorization functions and a preparation method thereof, and belongs to the technical field of cat litter. The high-performance mixed cat litter with the functional color development health indication and deodorization functions is prepared from the following raw materials in parts by mass: 3-7 parts of tofu cat litter, 3-7 parts of bentonite cat litter, 3-7 parts of efficient deodorization zeolite cat litter and 1-4 parts of functional color development health indication ceramsite, wherein the functional color development health indication ceramsite is prepared from the following raw materials in parts by mass: 85-92 parts of ceramsite, 0.3-1 part of rosin, 8-15 parts of absolute ethyl alcohol, 0.01-0.05 part of bromocresol green, 0.02-0.05 part of bromothymol blue and 0.06-0.1 part of phenophthalein. The high-performance mixed cat litter is good in water absorption and the caking property, low in dust content and high in deodorization efficiency and has a urine pH indication effect.

The invention relates to the technical field of ammonia nitrogen detection, in particular to a making method of a reaction film and an ammonia nitrogen detection device and method based on the reaction film. The making method includes: preparing a polyvinylidene chloride-bromothymol blue solution for coating, and coating optical fiber to form the reaction film on the surface of the covering of theinclined fiber Bragg grating. The ammonia nitrogen detection device comprises a light source, signal-mode optical fiber, an inclined fiber Bragg grating, a sample pool and a spectrograph, and the reaction film is plated on a covering of the inclined fiber Bragg grating. Light is adopted as a signal carrier, so that the ammonia nitrogen detection method has the advantage of high speed. The reaction film is simple in preparation process and low in cost. The ammonia nitrogen detection device is simple in structure, needless of a complex sample pool reaction system and high in detection accuracy.

The invention discloses a preparation method of an accurate indicating film for monitoring freshness of sashimi, relates to a preparation method of a freshness indicating film, and aims to solve the technical problems that the existing freshness indicating film is poor in mechanical property and unobvious in color development. The method comprises the steps of 1 preparing quaternized rice straw powder; 2 preparing grafted bromothymol blue rice straw powder; 3 adding carrageenan and sodium carboxymethyl cellulose into water, uniformly stirring, then adding the grafted bromothymol blue rice straw powder and sorbitol, and continuously stirring to obtain a film forming solution; and carrying out film casting on the film forming solution, and drying to obtain the accurate indicating film for monitoring the freshness of the sashimi. The indicating film can indicate the fresh degree of the sashimi in real time through four obviously different colors presented under different TVB-N values. The indicating film can be used in the intelligent label field.

The invention discloses a method for screening PHA producing bacteria. The method comprises the following steps: 1) manufacturing a culture medium; 2) enriching PHA producing bacteria; and 3) screening the PHA producing bacteria: spraying a bromothymol blue indicator into a culture medium plate, marking bluing bacterial colonies around the bacterial colonies, placing the bacterial colonies under an ultraviolet lamp for observation, and determining that the bacterial colonies are PHA producing bacteria if orange fluorescence appears; and picking the single colonies by using a sterilization gun head, performing smearing, placing the single colonies under blue light of a fluorescence microscope for observation and verification, then, picking the single colonies, performing oscillation and enrichment in a shaking table for 2 hours, dipping a small amount of bacterial liquid by using an inoculating loop for line drawing separation on a new plate culture medium, after inverse culture at 37 DEG C for 24 hours, picking the single colonies and performing numbering, and performing repeated purification and separation in such a way. According to the method, two indicators are selected from numerous indicators for screening PHA producing bacteria, and a one-step screening method is adopted, so that time and materials are saved; meanwhile, the false positive rate can be reduced; and the period required by purification and separation can be shortened.

The invention discloses a chromogenic medium of carbapenem resisting klebsiella, and belongs to the field of disease microorganism culturing and identification. The culture medium comprises 4-6 partsby weight of sodium chloride, 0.1-0.3 part by weight of magnesium sulphate, 0.5-1.5 parts by weight of ammonium dihydrogen phosphate, 0.5-1.5 parts by weight of dipotassium hydrogen phosphate, 4-6 parts by weight of sodium citrate, 13-17 parts by weight of agar, 0.06-1 part by weight of bromothymol blue, 9-11 parts by weight of myo-inositol, 0.001-0.003 part by weight of meropenem and 900-1100 parts by weight of water. The culture medium is good in specificity, quickly cultures the carbapenem resisting klebsiella, and facilitates infection control of the carbapenem resisting klebsiella in hospitals.