18 results about "Parahaemolyticus vibrio" patented technology

The application provides a bacillus desiccabilis strain XT23 microbial agent, a preparation method and application thereof, and belongs to the technical field of biological agents. A biological agent comprises a bacillus desiccabilis strain XT23, a fermentation treatment liquid of the bacillus desiccabilis strain XT23, or supernatant separated from the fermentation treatment liquid of the bacillus desiccabilis strain XT23. The fermentation treatment liquid is obtained by acid treatment and heat treatment of the fermentation liquid of the bacillus desiccabilis strain XT23, which effectively promotes spore formation, inactivates miscellaneous bacteria, significantly improves the stability and storage period of effective live bacteria, maximally retains antibacterial active substances in the fermentation liquid, and makes the inhibition effect of the final biological agent on Vibrio parahaemolyticus equivalent to that of fresh fermentation liquid. Meanwhile, the addition of the auxiliary material has a protective and slow-release effect on the active ingredients, and further enhances the convenience and effect persistence of application.

The application discloses a lytic bacteriophage vB_VpaS_R40Z and application thereof in preventing and controlling Vibrio parahaemolyticus in aquaculture environment. The Vibrio parahaemolyticus bacteriophage vB_VpaS_R40Z has a preservation number of GDMCC No: 67440-B1. The R40Z can lyse multiple strains of Vibrio parahaemolyticus and Vibrio alginolyticus, and has the ability to adapt to the aquaculture environment, and remains high activity within the range of 4-25 DEG C and pH 4-10; genome analysis does not detect virulence genes or drug resistance genes; phylogenetic comparison with the whole genome shows that the similarity of R40Z with the related bacteriophage is lower than the species level threshold, and R40Z is a new bacteriophage species; R40Z can significantly inhibit the growth of the host under multiple infection multiplicities. R40Z has the advantages of strong lytic ability, good environmental stability, high genome safety and unique evolutionary characteristics, and is a water product vibrio disease biological prevention and control bacteriophage with application potential.

The application discloses a colorimetric-SERS dual-mode detection and photothermal sterilization method for specifically detecting Vibrio parahaemolyticus and application thereof, and belongs to the technical field of food safety biology. The application takes Vibrio parahaemolyticus as a target, and prepares a multifunctional magnetic composite nanomaterial through layer-by-layer assembly. Firstly, the superparamagnetic separation performance of the magnetic composite nanomaterial is combined with magnetic chromatography to achieve the effect of rapidly separating bacteria from a complex food matrix. Secondly, the colorimetric and SERS sensing signals of the magnetic composite nanomaterial are used to quantitatively detect bacteria in a short time, high precision and high stability in a dual-mode. Finally, the photothermal performance of the magnetic composite nanomaterial is used to kill the detected bacteria by photothermal sterilization, so that the hazard control at the back end of detection is completed.

The invention relates to a primer probe group, a multiplex fluorescent quantitative PCR method and a kit for simultaneously detecting six pathogenic bacteria, and belongs to the technical field of molecular biology, the primer probe group comprises primer sequences and probe sequences shown in SEQ ID No.1-18 in a table 1; the 5'end of each probe sequence is modified with a reporter group, and the 3 'end of each probe sequence is modified with a quenching group; the reporter group is Cy5, FAM and VIC, and the quenching group is Eclipse. The invention provides a method for simultaneously detecting six pathogenic bacteria including vibrio parahaemolyticus, salmonella, staphylococcus aureus, escherichia coli O157: H7, listeria monocytogenes and shigella in the same reaction system by combining a multiple real-time fluorescent quantitative PCR (Polymerase Chain Reaction) technology. The multiplex real-time fluorescent quantitative PCR kit provided by the invention is used for detecting the six pathogenic bacteria, can improve the detection sensitivity and specificity, greatly improves the detection efficiency, and overcomes the defects of complex steps, long detection period and incapability of adapting to large-scale rapid detection in the conventional detection method.

This invention discloses a quadruple Taqman qPCR reagent for detecting foodborne pathogens in aquatic products. The foodborne pathogens are Vibrio parahaemolyticus, Vibrio vulnificus, Shigella, and Staphylococcus aureus. The reagent includes specific primers and Taqman probes corresponding to the four foodborne pathogens. This invention provides a quadruple Taqman qPCR reagent with high efficiency, accuracy, sensitivity, and speed, offering an accurate, rapid, efficient, and economical method for detecting the above four foodborne pathogens in the quality and safety testing of aquatic products and their processed products. This is of great significance for ensuring the safety of aquatic products for consumption in my country.

Primers and primer sets for amplification of Vibrio parahaemolyticus DNA and methods of detecting Vibrio parahaemolyticus using the primers and primer sets.

The present application relates to oligosaccharide composition and its use. The present application particularly relates to the use of oligosaccharide composition in the preparation of products for preventing and / or treating aquaculture diseases caused by Vibrio parahaemolyticus, Vibrio harveyi and Streptococcus agalactiae, wherein the oligosaccharide composition is obtained by degrading polygalacturonan or pectin by a polygalacturonase as shown in SEQ ID No: 1, under the conditions of a polygalacturonase concentration of 0.045 mg / mL to 0.055 mg / mL, a polygalacturonan concentration of 7% to 9%, and a degradation time of 12 hours to 20 hours. The present application also relates to the use of oligosaccharide composition in aquaculture. The oligosaccharide composition of the present application has good bacteriostatic effect and disease prevention and control effect in aquaculture.

The invention discloses construction and application of a vibrio parahaemolyticus capsular polysaccharide synthesis gene cluster and lipopolysaccharide synthesis HldE mutant strain, and belongs to the technical field of molecular biology and genetic engineering.According to the construction method, an overlapping extension PCR technology is adopted for constructing a kanamycin resistance gene knockout fusion fragment, the fusion fragment is cloned into a suicide plasmid pDS132, and the suicide plasmid pDS132 is used for preparing the HldE mutant strain of the vibrio parahaemolyticus capsular polysaccharide synthesis gene cluster and lipopolysaccharide synthesis HldE mutant strain. A recombinant suicide plasmid is introduced into vibrio parahaemolyticus through conjugational transfer, and sucrose-induced double-exchange homologous recombination and kanamycin resistance screening are utilized to efficiently obtain a capsular polysaccharide synthetic gene cluster and an HldE gene knockout mutant strain. The invention further discloses an application of the mutant strain in evaluating the exposure of the outer membrane protein, and discloses the influence of a capsule and a lipopolysaccharide layer on the accessibility of an outer membrane protein antibody. The method is easy and convenient to operate and high in screening efficiency, and an effective tool is provided for vibrio outer membrane protein function research, vaccine target screening and pathogenic mechanism analysis.

This invention discloses a molecular marker for programmed cell death protein in Litopenaeus vannamei and its application. The molecular marker includes molecular marker A, molecular marker B, molecular marker C, and molecular marker D located on the 6-like gene sequence of the programmed cell death protein in Litopenaeus vannamei. This invention utilizes these molecular markers as functional markers for Litopenaeus vannamei's resistance to Vibrio parahaemolyticus, thereby improving the disease resistance breeding of Litopenaeus vannamei. Specifically, genomic DNA is extracted from the muscle tissue of the Litopenaeus vannamei to be tested, and then used as template DNA for PCR amplification and purification of the PCR amplification products. The obtained products are then sequenced to determine the genotypes of molecular markers A, B, C, and D, thereby selecting individuals with superior genotypes for Litopenaeus vannamei breeding, promoting the research and application of disease-resistant aquaculture of Litopenaeus vannamei.

The invention provides a method for simultaneously and rapidly detecting vibrio parahaemolyticus and multiple drug-resistant genes thereof, which comprises the following steps: preparing vibrio parahaemolyticus immunomagnetic beads by coupling biotinylated vibrio parahaemolyticus polyclonal antibodies with streptavidin nano magnetic beads, and carrying out immunocapture on the vibrio parahaemolyticus. According to the present invention, the RPA-coupled micro-fluidic detection system is designed, the capture condition and the nucleic acid extraction process are optimized, the reasonable RPA-coupled micro-fluidic detection system is designed, the toxR gene of the vibrio parahaemolyticus and the three most common drug-resistant genes floR, sul1 and qnrA are synchronously detected, and the important significance is provided for solving the drug resistance problem of marine product pathogenic microorganisms and ensuring the human health.

This invention discloses the endolysin LysC75 of Vibrio parahaemolyticus phage and its applications. The amino acid sequence of endolysin LysC75 is shown in SEQ ID NO.2. The bactericide provided by this invention, with endolysin as the main active substance, can efficiently lyse Vibrio parahaemolyticus, with an effective bactericidal concentration of ≥0.2 mg / mL. When the bactericide with an endolysin concentration of 1 mg / mL is applied for 120 min, it can reduce the concentration of high-level Vibrio parahaemolyticus from 10... 8 CFU / mL dropped to 10 5 CFU / mL. A concentration of 1 mg / mL of this fungicide is effective against ≤10 3 The bactericidal effect of CFU / mL on Vibrio parahaemolyticus is 100%, and it is effective against 10... 6 -10 4 The sterilization rate of CFU / mL strains can reach over 90%, and for 10 9 -10 6 The bactericidal rate of CFU / mL strains can reach over 90%. This bactericide has high specificity against Vibrio parahaemolyticus and high lysis efficiency, providing an important alternative strategy for the control of drug-resistant Vibrio parahaemolyticus.

The application belongs to the technical field of microbial fermentation, and provides Lactobacillus paracasei subsp. paracasei with bacteriostatic and fermentation improvement characteristics, as well as a bacteriocin and application thereof. The classification name of the Lactobacillus paracasei subsp. paracasei is Lactobacillus paracasei subsp. paracasei, which has been preserved in the Guangdong Microbial Culture Collection Center (GDMCC) on June 25, 2025, the address of the preservation center is Building 59, 5th Floor, 100, Martyrs' Road, Guangzhou, and the preservation number is GDMCC No: 66588. The YCL29 bacteriocin produced by the application exhibits a significant inhibitory effect on Vibrio parahaemolyticus, and the starter prepared by the strain can be used to improve the flavor quality and metabolic regulation of fermented fish tea, thereby providing a new solution for developing a local starter with the functions of inhibiting raw material source pathogenic bacteria and regulating fermentation quality.

The invention discloses Chinese yam skin fermentation liquor as well as a preparation method and application thereof. The invention provides a plant lactobacillus Z165 strain with good environmental tolerance and safety in order to overcome the defect of lack of a strain for Chinese yam skin fermentation at present. By utilizing the plant lactobacillus Z165 strain, the Chinese yam peel can be effectively fermented, the antioxidant activity and the probiotic property of the obtained Chinese yam peel fermentation liquor are improved, and compared with Chinese yam peel slurry before fermentation or single plant lactobacillus Z165 strain, the antibacterial range and the activity of the obtained Chinese yam peel fermentation liquor are increased, so that the anti-oxidative activity of the Chinese yam peel fermentation liquor is improved, and the anti-oxidative activity of the Chinese yam peel fermentation liquor is improved. Various pathogenic bacteria such as escherichia coli, staphylococcus aureus, listeria monocytogenes and vibrio parahaemolyticus can be inhibited. The method is not only beneficial to the development and utilization of waste Chinese yam peel resources, but also beneficial to the development of natural antibacterial products.

The invention belongs to the technical field of biological medicine, and particularly relates to a pinocembrin and aminoglycoside medicine synergistic antibacterial composition and application thereof.The pinocembrin and aminoglycoside medicine are compounded, the sensitivity of the aminoglycoside medicine can be recovered, the toxic and side effects of antibacterial medicine can be remarkably reduced, and the antibacterial effect is good. The antibacterial activity on vibrio parahaemolyticus is improved, and a relatively good antibacterial effect is displayed. The pinocembrin and aminoglycoside drug compounded composition obtained by the invention has the advantages of low toxicity and high curative effect, and provides a new treatment strategy for clinical treatment of vibrio parahaemolyticus infectious diseases.

This invention provides a strain of *Bacillus desertica* XT23 resistant to *Vibrio parahaemolyticus*, its culture method, and its applications, belonging to the field of functional microbial technology. A strain of *Bacillus desertica* (… Bacillus inaquae Bacillus desertis strain XT23, with accession number GDMCC No: 67779, exhibits significant inhibitory effects against Vibrio parahaemolyticus, achieving an inhibition rate of 66.67%, demonstrating good potential for biocontrol. Optimization of the culture medium composition and fermentation process significantly increased bacterial biomass and the yield of antimicrobial active substances. The fermentation cycle was stable and reproducible, which is beneficial for further enhancing its activity against Vibrio parahaemolyticus.

The application discloses a method for identifying survival state of Vibrio parahaemolyticus based on split crRNA and application. The method comprises the following steps: incubating a to-be-detected sample containing target Vibrio parahaemolyticus mRNA with a split crRNA scaffold chain, an ssDNA activator, a Cas12a protein and a reporter probe at room temperature to obtain a reaction product; wherein the target Vibrio parahaemolyticus mRNA, the split crRNA scaffold chain and the ssDNA activator form a ternary complex, and the ternary complex can activate the Cas12a protein to cut the reporter probe and generate a fluorescence signal; and detecting the fluorescence signal intensity of the reaction product, so as to identify the survival state of Vibrio parahaemolyticus according to the fluorescence signal intensity. The method has the advantages of high sensitivity, good specificity and no need for nucleic acid amplification, and is suitable for efficient and reliable identification of live Vibrio parahaemolyticus.

The invention discloses a double-antibody sandwich ELISA kit for rapidly detecting vibrio parahaemolyticus and application of the double-antibody sandwich ELISA kit, an optimal pair 6D11 and 12B1 is obtained through preparation of monoclonal antibodies and screening of different antibody pairs, and a double-antibody sandwich ELISA method for the vibrio parahaemolyticus is established by taking the monoclonal antibody 6D11 as a coating antibody and taking 12B1-HRP as a detection antibody. The method has relatively high sensitivity (1.37 * 10 < 4 > CFU / mL) and relatively good specificity on vibrio parahaemolyticus, and has cross reaction on nine tested vibrio parahaemolyticus strains. And no cross reaction (27 / 29) exists on other 27 common intragenus and extragenus bacteria of vibrio, the detection limit of vibrio parahaemolyticus in seafood after enrichment for 6 hours is 10 CFU / mL, and the detection result is consistent with the detection result of a selective flat plate of a national standard method. The method provides an accurate, reliable and rapid analysis means for detection of vibrio parahaemolyticus in marine products.

The invention relates to the technical field of microorganisms, in particular to a penicillium oxalicum strain and preparation and application of a fermentation crude extract of the penicillium oxalicum strain, penicillium oxalicum D10 is obtained from mangrove forest rhizosphere soil, and detection shows that the fermentation crude extract of the penicillium oxalicum strain has a strong bacteriostatic effect on key vibrio pathogens. The minimum inhibitory concentrations (MIC) of the compound on vibrio parahaemolyticus, vibrio frenesii and vibrio alginolyticus are respectively 31.25 [mu] g / mL, 7.81 [mu] g / mL and 0.49 [mu] g / mL through determination by a trace double dilution method. The activity of the crude extract on vibrio alginolyticus is particularly prominent, more obvious high efficiency is achieved, active substances of the crude extract possibly derive from novel secondary metabolites and are different from an existing antibacterial mechanism, the problem of drug resistance is expected to be overcome, and high-quality germplasm resources are provided for later preparation of microecological preparation products.