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Preparation method of astragalus polysaccharide chitosan nanoparticles and application thereof

A technology of chitosan nanoparticles and astragalus polysaccharides, which is applied in the direction of medical preparations with non-active ingredients, medical preparations containing active ingredients, and pharmaceutical formulas, can solve the problem of no resistance to Edwardsiella catfish, degradation, and fast release speed. , Aquaculture fish inconvenience and other problems, to achieve the effect of reducing the number of administration and dosage, improving stability and reducing degradation

Pending Publication Date: 2020-01-10
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Astragalus polysaccharide is one of the main active components of Astragalus membranaceus. It has various pharmacological effects on the human body, such as immune regulation, anti-oxidation, anti-stress, liver and kidney protection, antibacterial, antiviral, etc. At present, astragalus polysaccharide has been widely used It is used in livestock and poultry production, and has also achieved good results in the prevention and control of some aquatic animal diseases, but there is no relevant report on the resistance to Edwardsiella catfish
In addition, the current conventional dosage forms of astragalus polysaccharides are degraded and released quickly, and most of the drugs have been degraded and metabolized before reaching the site of action. Therefore, it is necessary to increase the dosage and frequency of administration, which is very inconvenient for aquaculture fish.

Method used

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  • Preparation method of astragalus polysaccharide chitosan nanoparticles and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] 1) dissolving chitosan in 1% (v / v) acetic acid solution, making concentration after stirring and swelling is the chitosan acetic acid solution of 1.5mg / mL;

[0021] 2) adding astragalus polysaccharide to the chitosan acetic acid solution, stirring and dissolving, then adjusting the pH of the mixed solution to 5, and the mass ratio of chitosan and astragalus polysaccharide in the mixed solution is 6:1;

[0022] 3) Dissolve sodium tripolyphosphate with deionized water to make a sodium tripolyphosphate solution with a mass concentration of 1.5 mg / mL, and then add it dropwise to the mixed solution in step 2) while stirring, until the solution produces light blue When the color opalescent, stop dropping, continue to stir for 1h, obtain astragalus polysaccharide chitosan nanoparticle suspension;

[0023] 4) Divide the astragalus polysaccharide chitosan nanoparticle suspension into frozen bottles, pre-freeze at -70°C for 2 hours, and then vacuum freeze-dry at -50°C, and the ob...

Embodiment 2

[0025] 1) chitosan is dissolved in 1% (v / v) acetic acid solution, and after stirring and swelling, making concentration is the chitosan acetic acid solution of 3mg / mL;

[0026] 2) adding astragalus polysaccharide to the chitosan acetic acid solution, stirring and dissolving, then adjusting the pH of the mixed solution to 3, and the mass ratio of chitosan and astragalus polysaccharide in the mixed solution is 9:1;

[0027] 3) Dissolve sodium tripolyphosphate with deionized water to make a sodium tripolyphosphate solution with a mass concentration of 1 mg / mL, and then add it dropwise to the mixed solution in step 2) while stirring, until the solution turns light blue Stop the dropwise addition when opalescent, and continue to stir for 2 hours to obtain the astragalus polysaccharide chitosan nanoparticle suspension;

[0028] 4) Divide the astragalus polysaccharide chitosan nanoparticle suspension into frozen bottles, pre-freeze at -60°C for 3 hours, and then vacuum freeze-dry at ...

Embodiment 3

[0030] 1) dissolving chitosan in 1% (v / v) acetic acid solution, making concentration after stirring and swelling is the chitosan acetic acid solution of 0.5mg / mL;

[0031] 2) adding astragalus polysaccharide to the chitosan acetic acid solution, stirring and dissolving, then adjusting the pH of the mixed solution to 6, and the mass ratio of chitosan and astragalus polysaccharide in the mixed solution is 3:1;

[0032] 3) Dissolve sodium tripolyphosphate with deionized water to make a sodium tripolyphosphate solution with a mass concentration of 3 mg / mL, and then add it dropwise to the mixed solution in step 2) while stirring, until the solution turns light blue Stop the dropwise addition when opalescent, and continue to stir for 0.5h to obtain the astragalus polysaccharide chitosan nanoparticle suspension;

[0033] 4) Divide the astragalus polysaccharide chitosan nanoparticle suspension into freezing bottles, pre-freeze at -70°C for 3 hours, and then vacuum freeze-dry at -30°C,...

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Abstract

The invention discloses a preparation method of astragalus polysaccharide chitosan nanoparticles. The astragalus polysaccharide chitosan nanoparticles prepared by the method have good prevention and treatment effects on Edwardsiella catarrhalis. According to the preparation method, the astragalus polysaccharide chitosan nanoparticles are prepared by applying an ionic crosslinking method for the first time; the degradation of the medicine before reaching an acting part is reduced, the release speed of the medicine is reduced, the release time is prolonged, and the stability and in-vivo availability of astragalus polysaccharide are improved, so that the curative effect is improved, the administration frequency and dosage are reduced, and the product is more conveniently applied to fish culture.

Description

technical field [0001] The invention belongs to the field of pharmacy, and relates to a preparation method of astragalus polysaccharide-chitosan nanoparticles. The invention also relates to a new application of astragalus polysaccharide-chitosan in preventing and treating Edwardsiella catfish. Background technique [0002] Pelteobagrus fulvidraco belongs to Siluriformes, Bagridae, and Pelteobagrus. It is widely distributed in major water systems in my country, especially in lakes, ponds, and streams in the middle and lower reaches of the Yangtze River. distributed. As an important freshwater economic fish in my country, yellow catfish is delicious and tender, with excellent flavor and taste. It is favored by consumers and the market demand is increasing year by year. In recent years, it has been widely cultivated in China. However, with the continuous expansion of the artificial breeding area of ​​the yellow catfish, the accompanying disease problems are also becoming more a...

Claims

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Application Information

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IPC IPC(8): A61K31/715A61K9/19A61K47/36A61P31/04
CPCA61K31/715A61K9/19A61K47/36A61P31/04
Inventor 刘小玲陈会杰
Owner HUAZHONG AGRI UNIV
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