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Vaccine for preventing red head disease of Pseudobagrus fulvidraco

A technology for yellow catfish and vaccine, which is applied in the directions of medical preparations containing active ingredients, antibody medical ingredients, drug combinations, etc., can solve problems such as economic loss, influence on the quality of aquatic products, unclearness, etc., and achieves low cost and good effect. Effect

Active Publication Date: 2012-12-12
TONGWEI AGRI DEV CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the unclear etiology, once the disease occurs in production, drugs are used in a large encirclement method, which not only leads to an increase in treatment costs, but also leads to drug resistance due to the abuse of drugs. The treatment has brought great difficulties. At the same time, due to excessive drug use, the antibiotic residues in the fish body have exceeded the standard, which has an impact on the quality of aquatic products, resulting in serious economic losses and seriously hindering the healthy development of yellow catfish farming in my country.

Method used

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  • Vaccine for preventing red head disease of Pseudobagrus fulvidraco
  • Vaccine for preventing red head disease of Pseudobagrus fulvidraco
  • Vaccine for preventing red head disease of Pseudobagrus fulvidraco

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Embodiment one pathogenic bacteria Isolation and Identification of Edwardsiella ictaluri

[0025] 1 Materials and methods

[0026] 1.1 Test fish

[0027] Naturally occurring yellow catfish was collected from the Meishan yellow catfish farm in Sichuan; healthy yellow catfish was provided by Tongwei Co., Ltd. Sichuan Company, weighing 60.5±4.3g.

[0028] 1.2 Main reagents

[0029] Medium: TSA, TSB, TIS and BHI are self-made according to conventional methods.

[0030] Bacterial biochemical trace identification tube: purchased from Hangzhou Tianhe Biological Reagent Co., Ltd.

[0031] Taq DNA polymerase: purchased from Dalian Bao Biological Company.

[0032] PCR primers: synthesized by Dalian Bao Biological Company.

[0033] Drug-sensitive paper: purchased from Hangzhou Tianhe Biological Reagent Co., Ltd.

[0034] 1.3 Isolation and identification of bacteria

[0035] 1.3.1 Isolation of pathogenic bacteria: Sampling the brain and kidney of naturally infected fish by...

Embodiment 2

[0067] Embodiment two Comparative Study on the Standard Strain of Edwardsiella ictaluri and the CHNGY01 Strain Isolated by the Present Invention

[0068] 1 Materials and methods

[0069] 1.1 Source of bacteria

[0070] Strain CHNGY01 was isolated and identified from Tongwei Institute of Pharmaceutical Sciences from a yellow catfish farm in Meishan, Sichuan Edwardsiella, the deposit number is CCTCC NO: M209176.

[0071] The standard strain of Edwardsiella is ATCC 33202.

[0072] 1.2 Main reagents:

[0073] Bacterial biochemical trace identification tube: purchased from Hangzhou Tianhe Microbiological Reagent Co., Ltd.

[0074] Medium: Brain Heart Infusion (BHI) was purchased from Beijing Luqiao Technology Co., Ltd.

[0075] 1.3 Bacteria solution preparation

[0076] Before the test, use an inoculation loop to take the bacterial liquid from the glycerol bacterial liquid of the preserved strains, inoculate it in the brain heart infusion (BHI) broth medium, incubate at ...

Embodiment 3

[0087] Bacterial strain CHNGY01 of the present invention and Comparative Study on Inactivated Whole Bacteria Prepared by Edwardsiella Standard Strain (ATCC33202)

[0088] The "red head disease" of yellow catfish is a serious infectious disease that has occurred in recent years. The previous research has confirmed that its pathogen is Edwardsiella. In this experiment, the separation The comparative study on the immune effect of the inactivated vaccine prepared by Edwardsiella and the standard strain ATCC33202 on the red-head disease of yellow catfish provides a reliable basis for better immune prevention and treatment of red-head disease of yellow catfish.

[0089] 1. Materials and methods

[0090] 1.1 Strains

[0091] Yellow catfish Isolation and identification of Edwardsiella (CHNGY01) from yellow catfish naturally infected with red head disease

[0092] Edwardsiella Standard Strain (ATCC33202) ATCC Corporation

[0093] 1.2 Experimental fish

[0094]Healthy yell...

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Abstract

The invention provides application of Edwardsiella ictaluri to the preparation of fish vaccines and a fish vaccine. The fish vaccine comprises effective dose of immunogenic inactivated Edwardsiella ictaluri and pharmaceutically acceptable auxiliary materials or carriers. The vaccine or combined vaccine of the invention can pertinently prevent and treat red head disease of Pseudobagrus fulvidraco with good effect and is a low-cost and environmental-friendly prevention method.

Description

technical field [0001] The invention relates to a fish vaccine, in particular to a vaccine for preventing yellow catfish red head disease. Background technique [0002] Yellow catfish (Pelteobagrus fulvidraco), commonly known as yellow curd, belongs to the genus Pelteobagrus of the family Bagridae of the order Siluriformes. It has the characteristics of tender meat, delicious taste, no intermuscular spines, rich nutrition, low fat and high protein, and is very popular among consumers. Therefore, the cultivation of yellow catfish has developed rapidly throughout the country. increase yearly. However, in recent years, a kind of "red head disease" characterized by hyperemia, bleeding, redness, and even rupture of the head skin and the formation of open ulcers has occurred in various yellow catfish breeding areas. It is highly contagious and has the characteristics of high mortality. . At present, the etiology of the disease is unclear. Some farmers think it is a parasitic di...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K39/00A61P31/00
Inventor 肖丹罗远会方芳黄冠军耿毅邓龙君康琦刘衍鹏
Owner TONGWEI AGRI DEV CO LTD
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