Edwardsiella ictaluri colloidal gold immunochromatographic strip and preparation method thereof

A technology of immunochromatography test paper and colloidal gold, which is applied in the direction of analysis materials, measuring devices, instruments, etc., can solve the problem of no application of colloidal gold immunochromatography test strips, and achieve the effect of rapid detection, easy operation and high sensitivity

Active Publication Date: 2015-02-18
DALIAN OCEAN UNIV +1
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, since there is no monoclonal antibody against Edwardsiella spp. that is suitable for colloidal gold immunochromatographic test strips, microbiological detection techniques, immunofluorescence techniques, enzyme-linked immunosorbent assays, bacterial colloidal gold immunochromatographic test strips have not been used in the detection of Edwardsiella

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Edwardsiella ictaluri colloidal gold immunochromatographic strip and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0029] Edwardsiella colloidal gold immunochromatography test strip structure of the present invention is identical with prior art, has base plate 5, on base plate 5, is stuck with sample pad 4, gold standard pad 3, The nitrocellulose membrane 2 and the water-absorbing pad 1 overlap each other. The difference from the prior art is that the colloidal gold-labeled Edwardsiella monoclonal antibody is sprayed on the gold label pad 3, and the detection line a coated with the Edwardsiella polyclonal antibody is on the nitrocellulose membrane 2, and there is a goat anti-mouse The quality control line b composed of IgG, the Edwardsiella monoclonal antibody is prepared by immunizing mice with hybridoma cells with the preservation number CGMCC No. 7305, and purifying the ascites of the mice.

[0030] The hybridoma cell secreting the anti-Edwardia monoclonal antibody is prepared as follows:

[0031] 1. Preparation of bacterial liquid

[0032] Edwardsiella catarrhalis (ATCC 33202) was c...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses an edwardsiella ictaluri colloidal gold immunochromatographic strip which can rapidly detect edwardsiella ictaluri in aquatic animals and a preparation method thereof. The edwardsiella ictaluri colloidal gold immunochromatographic strip comprises a bottom plate, a sample pad, a conjugate pad, a nitrocellulose membrane and a water absorption pad are sequentially stuck on the bottom plate from one end from top to bottom in a stepped manner, a colloidal gold-labeled edwardsiella ictaluri monoclonal antibody is sprayed on the conjugate pad, a detection line a coated with an edwardsiella ictaluri polyclonal antibody and a quality control line b constituted by goat anti-mouse IgG are arranged on the nitrocellulose membrane, and the edwardsiella ictaluri monoclonal antibody is prepared by immunizing a mouse with a hybridoma cell strain Ei-5D11 of an anti-edwardsiella ictaluri monoclonal antibody with the collection number of CGMCC No. 7305, obtaining ascites of the mouse, and purifying.

Description

technical field [0001] The invention belongs to the technical field of rapid immunological detection of pathogenic bacteria in aquatic animals, in particular to an Edwardsiella colloidal gold immunochromatographic test strip capable of rapidly detecting Edwardsiella catalis in aquatic animals and a preparation method thereof. Background technique [0002] Edwardsiella ( Edwardsiella ictaluri) belonging to Enterobacteriaceae ( Enterobacteriaceae ), Edwardsiella ( Edward Siella ) is a pathogenic bacterium that causes Edwardsiculosis in a variety of fish. It is known that its infected hosts include channel catfish, yellow catfish, bearded catfish, European catfish, rainbow trout, black perch, etc., with acute onset, high morbidity and death Features such as high rate. At present, the detection methods for Edwardsiella mainly include traditional microbiological detection technology, immunofluorescence technology, enzyme-linked immunosorbent assay, bacterial agglutination m...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/577G01N33/569
Inventor 李强张显昱李华叶仕根黄华
Owner DALIAN OCEAN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap