Pseudobagrus-fulvidraco-richardson-origin transgenic vectors constructed with elements of pseudobagrus fulvidraco richardson [beta]-actin promoter and growth hormone gene, and application thereof

A technology of actin and yellow catfish, which is applied in the direction of using vectors to introduce foreign genetic material, recombinant DNA technology, etc.
CN102191270BInactive Publication Date: 2014-04-16NANJING UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
NANJING UNIV
Publication Date
2014-04-16
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a [beta]-actin promoter, a growth hormone gene and a 3<,>-untranslated region (3<,> UTR) of pseudobagrus fulvidraco richardson, and a pseudobagrus-fulvidraco-richardson-origin transgenic vector constructed with the [beta]-actin promoter, the growth hormone gene, and the 3<,> UTR as elements. According to the invention, sequences of the pseudobagrus fulvidraco richardson [beta]-actin promoter, sequences of exon 1, sequences of partial exon 2, amino acid data-encoding sequences of the growth hormone gene, and sequences of the 3<,> UTR are acquired. The pseudobagrus-fulvidraco-richardson-origin transgenic vector is constructed with the obtained [beta]-actin promoter, the obtained growth hormone gene, and the obtained 3<,> UTR as elements. According to the invention, the transgenic vector is constructed through driving the growth hormone gene expression of pseudobagrus fulvidraco richardson by the [beta]-actin promoter of its own. With the technical scheme of the present invention, biosafety problems of faced by transgenic fish can be effectively avoided. Therefore, the invention has great practical significance and application prospects in the genetic breeding and artificial fertilizing of pseudobagrus fulvidraco richardson.
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Description

technical field

[0001] The present invention relates to the cloning, recombination and promoter activity analysis and application of the promoter of the β-actin of the yellow catfish, the growth hormone gene and the 3'-untranslated region sequence, and constructs the original transgene of the yellow catfish as an element The carrier belongs to the field of genetic engineering. Background technique

[0002] Yellow catfish (Pseudobagrus fulvidraco Richardson) is commonly known as Angzi, yellow wax ding. Belongs to Siluriformes, Cullidae, Peltridae. The fish is a warm-water fish that lives on the bottom. The individual is usually about 30-100 grams, and it is widely distributed in inland waters of my country. Its body surface is smooth and phosphorus-free, the meat is tender, without intermuscular spines, the protein content is 15.37%, the total amino acid content is 14.19%, the essential amino acid index reaches 73.34, the lysine content is high, the taste is delicious, and ...

Claims

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