Taxus chinensis var mairei cultivation method
A technology of southern yew and its cultivation method, which is applied in the cultivation field of southern yew, can solve the problems of slow growth rate, long growth cycle, poor regeneration ability, etc., and achieve the effects of high rooting rate, short cultivation time and low cost
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[0023] 1. Tissue culture of Taxus chinensis
[0024] ①Collect the branches of the southern yew in the same year, wash them repeatedly, and carry out hydroponics. When the shoots grow to 1-2cm, cut them off with scissors and put them in a beaker. Cover the mouth of the beaker with gauze, and pour clean water for 2.2 hours; In alcohol with a concentration of 75%, take it out after 30 seconds, rinse it with sterile water for 3 times, put it in 84 disinfectant solution with a concentration of 4% for 7 minutes, and wash it with sterile water for 5 times; Bacteria operation, cut off the leaves on the shoots, and cut the stems of the shoots into 1.2 cm long stems with internodes, inoculate the stems in selenium-enriched MS medium at a temperature of 25°C 16 hours of light per day and an initial culture of 12 days in an environment with a light intensity of 750lux. The selenium-enriched MS medium is prepared by adding 1 / 100,000 inorganic selenium to the traditional MS medium;
[0025...
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