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Taxus chinensis var mairei cultivation method

A technology of southern yew and its cultivation method, which is applied in the cultivation field of southern yew, can solve the problems of slow growth rate, long growth cycle, poor regeneration ability, etc., and achieve the effects of high rooting rate, short cultivation time and low cost

Inactive Publication Date: 2014-07-02
HUNAN LYUBO AGRI & FORESTRY DEV CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the natural environment, southern yew generally has the problems of slow growth rate, long growth cycle, poor regeneration ability, and low survival rate. Therefore, for a long time, there has not been a large-scale southern yew raw material forest cultivation base in the world.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0023] 1. Tissue culture of Taxus chinensis

[0024] ①Collect the branches of the southern yew in the same year, wash them repeatedly, and carry out hydroponics. When the shoots grow to 1-2cm, cut them off with scissors and put them in a beaker. Cover the mouth of the beaker with gauze, and pour clean water for 2.2 hours; In alcohol with a concentration of 75%, take it out after 30 seconds, rinse it with sterile water for 3 times, put it in 84 disinfectant solution with a concentration of 4% for 7 minutes, and wash it with sterile water for 5 times; Bacteria operation, cut off the leaves on the shoots, and cut the stems of the shoots into 1.2 cm long stems with internodes, inoculate the stems in selenium-enriched MS medium at a temperature of 25°C 16 hours of light per day and an initial culture of 12 days in an environment with a light intensity of 750lux. The selenium-enriched MS medium is prepared by adding 1 / 100,000 inorganic selenium to the traditional MS medium;

[0025...

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PUM

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Abstract

The invention provides a taxus chinensis var mairei cultivation method. The taxus chinensis var mairei cultivation method comprises the following steps: firstly carrying out tissue culture on taxus chinensis var mairei so as to obtain test-tube plantlets, hardening off the test-tube plantlets, and then carrying out cuttage. The taxus chinensis var mairei cultivation method has the beneficial effects that 1. selenium is added to a tissue culture medium and nutrient soil, and the growth of the taxus chinensis var mairei can be effectively promoted in a tissue cultivation process and in a cuttage process; 2. the tissue cultivation method is simple to operate, the technical scheme is mature, the cultivation time is short, the cost is low, the survival rate is high, the rooting percentage is high, and the bottle-out transplantation can be realized in short time; 3. the tissue cultivation method is capable of maintaining good characters of original varieties and lays a good foundation for taxus chinensis var mairei breeding and agricultural modernization; 4. the cuttage method is simple in operation steps, high in survival rate and applicable to most of regions in Southern China and has a good application effect.

Description

technical field [0001] The invention relates to the field of plant cultivation, in particular to a method for cultivating the southern yew. Background technique [0002] Southern yew is an evergreen tree, the branchlets turn yellow-green or light reddish brown in autumn, the leaves are strip-shaped, dioecious, and the seeds are flat and round. The seeds are used to extract oil and can also be used as medicine. It is a shallow-rooted plant with inconspicuous main roots and well-developed lateral roots. It is recognized as an endangered natural and rare anti-cancer plant in the world. It is an ancient tree species left over from Quaternary glaciers and has a history of 2.5 million years on the earth. In the natural environment, southern yew generally has the problems of slow growth, long growth cycle, poor regeneration ability, and low survival rate. Therefore, for a long time, there has not been a large-scale cultivation base of southern yew raw material forests in the w...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01G1/00
Inventor 杨江波
Owner HUNAN LYUBO AGRI & FORESTRY DEV CO LTD
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