82results about How to "Maintain traits" patented technology

The invention discloses a synchronous denitration process based on wet ammonia process flue gas desulfurization. The problems such as high operating cost and equipment investment cost, complex process and high energy consumption existing in an existing process are solved. According to the technical scheme, the process comprises the following steps: pressurizing flue gas, feeding the flue gas into a concentrating tower, allowing the flue gas to contact and react with a concentrated solution in the tower, feeding the flue gas out of the concentrating tower into an absorption tower to contact and react with absorption liquid in the tower, feeding a small amount of reacted concentrated solution at the bottom of the concentrating tower into an ammonium sulfate crystallization system, and adding a complexing agent EDTA-Fe (II) or adding EDTA-Na salt and ferrous sulfate according to a molar ratio of 1:1 into the absorption liquid in the absorption tower, so that the concentration of the EDTA-Fe (II &III) in the absorption liquid is 0.015-0.05mol/L; and feeding a small amount of absorption liquid in the absorption tower into the concentrating tower to be mixed with the concentrated solution. The synchronous denitration process disclosed by the invention has the advantages of simplicity, low operating cost and equipment investment, capacity of performing high-efficiency synchronous desulfurization and denitration and low energy consumption.

The invention provides a taxus chinensis var mairei cultivation method. The taxus chinensis var mairei cultivation method comprises the following steps: firstly carrying out tissue culture on taxus chinensis var mairei so as to obtain test-tube plantlets, hardening off the test-tube plantlets, and then carrying out cuttage. The taxus chinensis var mairei cultivation method has the beneficial effects that 1. selenium is added to a tissue culture medium and nutrient soil, and the growth of the taxus chinensis var mairei can be effectively promoted in a tissue cultivation process and in a cuttage process; 2. the tissue cultivation method is simple to operate, the technical scheme is mature, the cultivation time is short, the cost is low, the survival rate is high, the rooting percentage is high, and the bottle-out transplantation can be realized in short time; 3. the tissue cultivation method is capable of maintaining good characters of original varieties and lays a good foundation for taxus chinensis var mairei breeding and agricultural modernization; 4. the cuttage method is simple in operation steps, high in survival rate and applicable to most of regions in Southern China and has a good application effect.

A tissue culture method for clematis Multi-Blue includes such steps as disinfecting the stem tip of clematis multi-blue by alcohol or corrosive sublimate, flushing with aseptic water, primary culture, secondary culture, rooting culture and reproducing embryoids.

The invention provides a recombinant human collagen and a medical nanometer fibrous membrane thereof. Besides, the invention also provides a preparation method for the medical nanometer fibrous membrane. The method comprises the following steps: mixing and reacting recombinant human collagen, polyethylene oxide and carbodiimide with chitosan and then spinning.

The invention discloses a cuttage seedling raising method for twigs of ilex chinensis Sims. According to the method, nursery stocks of ilex chinensis Sims are bred by utilizing the cuttage of the twigs of fruiting ilex chinensis Sims, the genetic characteristics of excellent mother plants of ilex chinensis Sims can be kept, and rapid breeding and cultivation of ilex chinensis Sims are achieved. The method comprises the steps of selection of mother trees, management, protection and cultivation of the mother trees, collection of cuttings, treatment of the cuttings, cuttage of the cuttings, infusion feeding for scions and management after cuttage, wherein the robust and disease-free fruiting nursery stocks are used as the mother trees, a cutting orchard is established, the twigs are cut for sprouting promotion, semi-lignified branches are selected as the cuttage scions, the cuttings are subjected to rooting-promotion and non-destructive treatment, the scions are supplied with nutrients invitro after cuttage, through tests, the rooting survival rate of the cuttings is 96%, and a large number of nursery stocks of ilex chinensis Sims can be quickly bred. The unique methods and structures of pretreatment of the scions and infusion feeding for the scions are critical, the problem of difficult breeding of seeds of ilex chinensis Sims is solved, the breeding speed is high, and the method is simple.

The invention provides a magnolia liliiflora tissue culture method. The magnolia liliiflora tissue culture method is performed under open conditions, and the method comprises the following steps: performing sterilization and bacteriostasis on a magnolia liliiflora tissue culture field, selecting an explant, sterilizing and disinfecting the explant, performing primary culture on the explant, performing multiplication culture and performing rooting culture. The magnolia liliiflora tissue culture method is performed under open conditions and can be performed under the open conditions, and the cost is saved; according to sterilization of the magnolia liliiflora, the explant is not contaminated by bacteria, and the culture survival rate is improved; the explant is soaked by 2 percent of PVP, and the 2 percent of PVP is added into a culture medium, so that browning of the explant is lightened, and the survival rate is improved. The magnolia liliiflora tissue culture method is simple in operation, short in time, low in cost, high in survival rate and high in rooting rate, bottle transplanting in a short time can be realized, desirable traits of the original variety can be kept, and a foundation is laid for seed stock breeding and industrialized production.

The invention discloses an isolated adipose protectant which comprises normal saline, DMEM solution, L-glutamine, penicillin and streptomycin according to the proportion of 500ml to 500ml to (146.1mg-584.4mg) to 125000U to 125000U. The invention further discloses a preparation method and application of the protectant. The protectant has effect of effectively reducing adipose tissue culture stem cell contamination rate. Growth rate of the stem cells during culture of the adipose tissue is higher than that of stem cells growing without protective liquid, and culture time of primary cells can be shortened, culture efficiency is improved, and cost is saved.

A detoxification method for an edible fungus strain comprises the following steps: making a potato dextrose agar (PDA) culture medium in a conventional method, utilizing chloramphenicol to process the surface of the culture medium, adding the to-be-detoxified strain, and performing conventional culture; when hyphae grow to 1/2, adding a secondary culture medium in the conventional method to perform culture, extracting a primordium tissue to add the PDA culture medium, and performing culture in the conventional method; when the hyphae grow to 1/2, picking mycelia at the 2-3mm positions of tips of the hyphae, adding the PDA culture medium, and performing culture in the conventional method; adopting a primordium separation method and a tip separation method to perform cyclic operation for 2-4 times, and achieving the aim of detoxification. Original biological characteristics and production traits of the detoxified strain can be recovered completely, stress resistance and disease resistance are obviously reinforced, the morbidity is reduced by over 60% in comparison with conventional strains, the seed production survival rate of original seeds and cultivated seeds can be improved to over 95%.

The invention discloses a method for preparing ageratum oral liquid. The active ingredients of most of prescription medicinal crops are extracted by using a membrane press filtration, cyclic extraction and separation integrated technology in place of a reflux method, a decoction method and a warm immersion method. A process flow can be simplified, the production period can be greatly shortened, energy can be saved, environmental protection and waste residue utilization can be facilitated, and the recovery rate of the active ingredients can be greatly increased.

The invention discloses a medical disinfecting and sterilizing ultrasonic coupling agent and a preparation method thereof. The ultrasonic coupling agent comprises 0.1 to 2 percent of R-polysaccharide, 2 to 7 percent of propylene glycol, 2 to 7 percent of glycerol, 0.6 to 1.5 percent of crosslinking (polyacrylic acid) resin, and 82.8 to 95.3 percent of distilled water; and in addition, triethanolamine is added in an amount which is 0.8 to 1.2 percent based on the total weight of the above materials. The preparation method comprises the following steps: preparing mixed solution of propylene glycol and glycerol; completely dissolving the R-polysaccharide in the prepared mixed solution; preparing solution of crosslinking (polyacrylic acid) resin; adding the prepared mixed solution containing the R-polysaccharide into the solution of crosslinking (polyacrylic acid) resin to obtain a gelatinous polymer, supplementing distilled water and stirring the solution uniformly; and slowly dripping solution of triethanolamine to neutralize the obtained ultrasonic coupling agent. In the invention, the problem that effective disinfection cannot be realized in a short time is solved and the preparation process is simple.

The invention discloses a beautifying ultrasonic coupling agent and a preparation method and application thereof, and belongs to the technical field of ultrasonic beautifying. The beautifying ultrasonic coupling agent is prepared from 0.4-2% of gel, 5-10% of a moisturizing agent, 5-15% of beautifying plant essence, 0.4-0.8% of an antioxidant and 72.2-89.2% of water. The beautifying ultrasonic coupling agent selects various natural plant formulas, an ultrasonic probe can be isolated from skin, ultrasonic energy is transmitted into the deep skin, and cell regeneration is stimulated; meanwhile, the beautifying ultrasonic coupling agent has rich beautifying plant essence components which are guided into the skin through the ultrasonic energy, the exponentially improved beautifying effect is generated under the mutual effect of the ultrasonic energy and the beautifying plant essence, and the problems that the beautifying effect obtained when ultrasonic wave is independently used for beautifying or only make-up appliances are used for beautifying is not ideal are solved.

The invention relates to a method for improving quality of cotton fibers by overexpressing GhCAD6 gene, belonging to the technical field of biology. The method comprises the following steps: constructing a plant overexpression vector pCAMINBIA-2300-GhCAD6 from styrone dehydrogenase gene GhCAD6 in cotton fibers, transforming the vector into agrobacterium LB4404 by agrobacterium mediation, and integrating into cotton to obtain the trans-styrone dehydrogenase gene cotton plant. The field and molecular detection analysis detects that the lignin content in cotton fibers obviously increases in different development periods, the fibrocyte wall thickness obviously decreases in the cotton secondary wall rapid deposition stage, and the ripe cotton fiber surface becomes more compact and smooth. The method can maximally enhance the length of the cotton fibers by 16.935%, enhance the specific strength by 25.10% and enhance the fiber yield by 3.80%, thereby greatly improving the quality of the cotton fibers.

The invention provides a lymphatic endothelial cell culture medium. The culture medium comprises a basic culture medium and an additive, and the additive comprises an insulin growth factor, a basic fibroblast growth factor, an epidermal growth factor, a vascular endothelial growth factor, vitamin C, transferrin, bovine serum albumin, cortisol, penicillin and streptomycin. The cell culture medium provided by the invention effectively promotes proliferation of lymphatic endothelial cells. According to the invention, the growth factors and the components are matched and added, properties of primary lymphatic endothelial cells can be effectively maintained, and after expanding for eight generations in vitro, the cells can still maintain original properties to a large extent, so that the survival time is prolonged. The components added in the culture medium are easy to obtain, preparation is convenient, and cost for culturing endothelial cells is reduced.

The invention discloses a method for extracting laminarin from kelp. The method comprises the steps as follows: raw material treatment, ultrasonic treatment, enzymolysis, decolorization, deproteinization and drying. During raw material treatment, enzymolysis is performed after ultrasonic treatment, the characters of proteoglycan protein can be well kept while raw material gelling and denaturationof proteoglycan protein are caused by long-term high-temperature heating treatment in the prior art, and the method can effectively improve the laminarin extraction efficiency as compared with the prior art.

The invention discloses an in vitro preservation culture medium for cassava embryogenic callus. A formula of the in vitro preservation culture medium is that an improved GD culture medium is added with 0.6-0.8mg/L of 2,4-D, 6-7mg/L of LTIBA, 20-25g/L of saccharose and 6.0-6.5g/L of agar. The in vitro preservation culture medium is placed under conditions with a temperature of 13-17 DEG C, illumination of 100-1200Lx and illumination time of 16-18h/d to perform culturing. The culture medium is adopted to carry out in vitro preservation on the cassava embryogenic callus, has embryogenic callus multiplication amount which is 1.0-1.2 times that of a traditional culture medium, cell activity which is 2-4 times that of the traditional culture medium and the best preserving effect when subculture time is prolonged to 120d. The method is adopted to carry out in vitro preservation for cassava embryogenic callus, so that basis is provided for storing cassava germplasm resources.

The invention discloses a high-efficiency preparation method of human skin tissue pluripotent stem cells. The method comprises the following steps: preparation of coating bottle: putting a coating bottle in a 4-DEG C refrigerator, and storing for later use; and preparation of skin stem cells: taking a cheek or chin skin tissue, removing the fat tissue, putting the tissue block in a sterile plate, digesting over night, digesting corium layer small blocks, filtering the cell suspension by a filter, keeping the adherent cells, culturing in an incubator, repeatedly beating upon the bottle bottom by using a suction tube until the cells shed, carrying out bottle subculture on the cells according to the ratio of 1:3, and continuing culturing by 2-5 generations. The method has the advantages of high cell yield, high differentiation efficiency and the like.

The invention discloses a preparation method of a yogurt starter culture, and belongs to the technical field of foods. According to the invention, fruit juice is added, and various fruits contain components which are beneficial to the human bodies so as to further increase the efficacy of yogurt. Meanwhile, various sugars are added into a nutrient solution, and a sugar content is high so as to notonly provide nutrient fermentation with a better nutrient environment and improve the activity of strains, but also accelerate a fermentation rate. Besides, the two strains adopted by the invention promote the growth of all parties. Meanwhile, defatted milk powder and an antioxidant are added to form a carrier protective agent, so that the carrier protective agent can enter the interior of cellsand prevent freezing injuries inside and outside the cells. A mixture is subjected to pre-freezing, and gets into a solid state so as to avoid the inactivation of active macromolecules in a process ofvacuum freeze-drying and maintain the original characteristics of the strains. The method solves problems of some existing yogurt starter cultures that a flavor is not heavy enough and sourness is too strong, which result in a poor mouth feel and the need of adding sweeteners.