82results about How to "Maintain traits" patented technology

The invention discloses a synchronous denitration process based on wet ammonia process flue gas desulfurization. The problems such as high operating cost and equipment investment cost, complex process and high energy consumption existing in an existing process are solved. According to the technical scheme, the process comprises the following steps: pressurizing flue gas, feeding the flue gas into a concentrating tower, allowing the flue gas to contact and react with a concentrated solution in the tower, feeding the flue gas out of the concentrating tower into an absorption tower to contact and react with absorption liquid in the tower, feeding a small amount of reacted concentrated solution at the bottom of the concentrating tower into an ammonium sulfate crystallization system, and adding a complexing agent EDTA-Fe (II) or adding EDTA-Na salt and ferrous sulfate according to a molar ratio of 1:1 into the absorption liquid in the absorption tower, so that the concentration of the EDTA-Fe (II &III) in the absorption liquid is 0.015-0.05mol / L; and feeding a small amount of absorption liquid in the absorption tower into the concentrating tower to be mixed with the concentrated solution. The synchronous denitration process disclosed by the invention has the advantages of simplicity, low operating cost and equipment investment, capacity of performing high-efficiency synchronous desulfurization and denitration and low energy consumption.

The invention discloses a method for semi-lignified twig cutting breeding of Aquilaria sinensis, which comprises: collecting wide high-quality Aquilaria sinensis seeds, sowing the seeds for raising seedlings and building a cutting orchard with high-quality seedlings; in the following year, trimming the seedlings in the cutting orchard at the place which is more than or equal to 15 centimeters away from the ground, and applying 6-BA on the trimmed seeding stems for promoting the germination of twigs; collecting materials when the twigs grow to a semi-lignified state by cutting the twigs to 10 to 15 centimeters with three top leaves left and each leaf having 2 / 5 leaf area; cutting the cuttingwood with a flat cut, obliquely cutting the lower end of the cuttingwood at a position as close to leaf segment as possible; cleaning the cutting wood, disinfecting, plugging the lower part of the cutting wood into prepared plant growth regulator treatment solution, inserting the cutting wood into a medium, performing post-cuttage management and obtaining Aquilaria sinensis seedlings. The method is low in cost, ensures high survival rate, high seeding quality and quick propagation, relieves seed shortage situation, keeps the excellent hereditary characters of the high-quality trees, is easy to promote and has a promising development prospect.

The invention provides a taxus chinensis var mairei cultivation method. The taxus chinensis var mairei cultivation method comprises the following steps: firstly carrying out tissue culture on taxus chinensis var mairei so as to obtain test-tube plantlets, hardening off the test-tube plantlets, and then carrying out cuttage. The taxus chinensis var mairei cultivation method has the beneficial effects that 1. selenium is added to a tissue culture medium and nutrient soil, and the growth of the taxus chinensis var mairei can be effectively promoted in a tissue cultivation process and in a cuttage process; 2. the tissue cultivation method is simple to operate, the technical scheme is mature, the cultivation time is short, the cost is low, the survival rate is high, the rooting percentage is high, and the bottle-out transplantation can be realized in short time; 3. the tissue cultivation method is capable of maintaining good characters of original varieties and lays a good foundation for taxus chinensis var mairei breeding and agricultural modernization; 4. the cuttage method is simple in operation steps, high in survival rate and applicable to most of regions in Southern China and has a good application effect.

A tissue culture method for clematis Multi-Blue includes such steps as disinfecting the stem tip of clematis multi-blue by alcohol or corrosive sublimate, flushing with aseptic water, primary culture, secondary culture, rooting culture and reproducing embryoids.

The invention relates to a quick-frozen dumpling in soup which can prevent soup from blending, the method for preparing the skin of the dumpling in soup is to putting the powder of 100 units for preparing the dumpling block into basin, adding edible glue 0.1-30 units, mixing them uniformly, adding water of 15-40 units to make flour dough, then choosing the stuffing material for dumpling, making dumpling according to the ratio of 0.5:1-3:1 between skin and stuffing, quick freezing under temperature not below five Deg C below zero, and storing. Because of the containing of edible glue, the dumpling is characterized by preventing soup from blending, the shape maintaining during boiling, and the taste equal to that of not frozen.

The invention discloses a method for cutting seedling-raising of Alnus trabeculosa. The method is characterized by comprising the following steps: (1), collection and treatment of a stalk for cutting; (2), arrangement of a cutting bed; and (3), cutting on the cutting bed. The cutting method provided by the invention has the advantages that the rooting rate of Alnus trabeculosa is high, original properties of plants can be retained, propagation of Alnus trabeculosa is accelerated, and the market demand for garden greening is satisfied.

The invention provides a recombinant human collagen and a medical nanometer fibrous membrane thereof. Besides, the invention also provides a preparation method for the medical nanometer fibrous membrane. The method comprises the following steps: mixing and reacting recombinant human collagen, polyethylene oxide and carbodiimide with chitosan and then spinning.

The invention discloses a cuttage seedling raising method for twigs of ilex chinensis Sims. According to the method, nursery stocks of ilex chinensis Sims are bred by utilizing the cuttage of the twigs of fruiting ilex chinensis Sims, the genetic characteristics of excellent mother plants of ilex chinensis Sims can be kept, and rapid breeding and cultivation of ilex chinensis Sims are achieved. The method comprises the steps of selection of mother trees, management, protection and cultivation of the mother trees, collection of cuttings, treatment of the cuttings, cuttage of the cuttings, infusion feeding for scions and management after cuttage, wherein the robust and disease-free fruiting nursery stocks are used as the mother trees, a cutting orchard is established, the twigs are cut for sprouting promotion, semi-lignified branches are selected as the cuttage scions, the cuttings are subjected to rooting-promotion and non-destructive treatment, the scions are supplied with nutrients invitro after cuttage, through tests, the rooting survival rate of the cuttings is 96%, and a large number of nursery stocks of ilex chinensis Sims can be quickly bred. The unique methods and structures of pretreatment of the scions and infusion feeding for the scions are critical, the problem of difficult breeding of seeds of ilex chinensis Sims is solved, the breeding speed is high, and the method is simple.

The invention relates to a method for rapid breeding by pinellia ternate stems, and belongs to the field of traditional Chinese medicine resources. The method has a short breeding period. The method comprises the following steps of a, inoculation and b, induced culture. The method utilizes non-medicinal parts of pinellia ternate to carry out tissue culture, has a fast breeding rate and a low production cost, and is suitable for large-scale, fast and normalized breeding of pinellia ternate seeds. The invention also provides a culture box for pinellia ternate breeding. The culture box comprises a box body and a box cover. The box body and the box cover are quadrate. The box cover is provided with an air port. An air port cover is arranged on the air port. The air port is in a threaded connection relationship with the air port cover. The culture box for pinellia ternate breeding can fully utilize a space, is conducive to air circulation and can improve breeding effects.

The invention provides a magnolia liliiflora tissue culture method. The magnolia liliiflora tissue culture method is performed under open conditions, and the method comprises the following steps: performing sterilization and bacteriostasis on a magnolia liliiflora tissue culture field, selecting an explant, sterilizing and disinfecting the explant, performing primary culture on the explant, performing multiplication culture and performing rooting culture. The magnolia liliiflora tissue culture method is performed under open conditions and can be performed under the open conditions, and the cost is saved; according to sterilization of the magnolia liliiflora, the explant is not contaminated by bacteria, and the culture survival rate is improved; the explant is soaked by 2 percent of PVP, and the 2 percent of PVP is added into a culture medium, so that browning of the explant is lightened, and the survival rate is improved. The magnolia liliiflora tissue culture method is simple in operation, short in time, low in cost, high in survival rate and high in rooting rate, bottle transplanting in a short time can be realized, desirable traits of the original variety can be kept, and a foundation is laid for seed stock breeding and industrialized production.

The invention discloses a wet flue gas ammonia desulfurization-based synchronous denitration system, and aims at solving the problems that an existing system is high in operation cost, high in equipment investment cost, complicated in process and high in energy consumption. According to the technical scheme, the synchronous denitration system comprises a concentration tower and an absorption tower, which are connected with each other through a flue gas pipeline; a circulating hole is formed in the bottom of the absorption tower, and is communicated with the upper part of the absorption tower through an absorption liquid circulating spray line; a drainage hole in the bottom of the absorption tower is connected with the concentration tower via a pipe; a liquid outlet in the bottom of the concentration tower is connected with a first settling pond through a first de-ironing reaction tank; a clear liquid outlet in the upper section of the first settling pond is connected with an ammonium sulfate crystallization system. The wet flue gas ammonia desulfurization-based synchronous denitration system has the advantages of low operation cost, low equipment investment and low energy consumption, and can be used for carrying out desulfurization and denitration efficiently and synchronously.

The invention discloses an isolated adipose protectant which comprises normal saline, DMEM solution, L-glutamine, penicillin and streptomycin according to the proportion of 500ml to 500ml to (146.1mg-584.4mg) to 125000U to 125000U. The invention further discloses a preparation method and application of the protectant. The protectant has effect of effectively reducing adipose tissue culture stem cell contamination rate. Growth rate of the stem cells during culture of the adipose tissue is higher than that of stem cells growing without protective liquid, and culture time of primary cells can be shortened, culture efficiency is improved, and cost is saved.

The invention relates to a medical ultrasonic coupling agent with disinfection and sterilization functions. The weight percentage of the ingredients is: 0.2-2% of 2,4,4'-trichloro-2'-hydroxydiphenyl ether, 4-7% of glycerol, Cross-linked polyacrylic acid resin 0.6-1.5%, sodium hydroxide 0.8-1.2%, distilled water 89.0-94.0%. The preparation steps are as follows. In the first step, 2,4,4'-trichloro-2'-hydroxydiphenyl ether and glycerol are weighed by weight percentage, and 2,4,4'-trichloro-2'- The hydroxydiphenyl ether is completely dissolved in the glycerin solution and left in place; in the second step, the cross-linked polyacrylic acid resin is weighed by weight percentage and dissolved in distilled water; the solution obtained in the first step is added into the second step under stirring to obtain The solution was then supplemented with distilled water and sodium hydroxide solution to make a gel. The invention can sterilize the ultrasonic probe during the inspection process, reducing cross infection; the preparation process is simple, and the environment is not polluted.

A detoxification method for an edible fungus strain comprises the following steps: making a potato dextrose agar (PDA) culture medium in a conventional method, utilizing chloramphenicol to process the surface of the culture medium, adding the to-be-detoxified strain, and performing conventional culture; when hyphae grow to 1 / 2, adding a secondary culture medium in the conventional method to perform culture, extracting a primordium tissue to add the PDA culture medium, and performing culture in the conventional method; when the hyphae grow to 1 / 2, picking mycelia at the 2-3mm positions of tips of the hyphae, adding the PDA culture medium, and performing culture in the conventional method; adopting a primordium separation method and a tip separation method to perform cyclic operation for 2-4 times, and achieving the aim of detoxification. Original biological characteristics and production traits of the detoxified strain can be recovered completely, stress resistance and disease resistance are obviously reinforced, the morbidity is reduced by over 60% in comparison with conventional strains, the seed production survival rate of original seeds and cultivated seeds can be improved to over 95%.

The invention discloses a method for preparing ageratum oral liquid. The active ingredients of most of prescription medicinal crops are extracted by using a membrane press filtration, cyclic extraction and separation integrated technology in place of a reflux method, a decoction method and a warm immersion method. A process flow can be simplified, the production period can be greatly shortened, energy can be saved, environmental protection and waste residue utilization can be facilitated, and the recovery rate of the active ingredients can be greatly increased.

The invention discloses an extraction method for farmland soil microplastics, which comprises the following steps: adding a flotation solution into a pretreated soil sample, uniformly stirring, and standing to obtain a precipitation layer and a non-precipitation layer which are distributed in a layered manner; attaching the surface of the metal container attached with the oil film to the surface of the non-precipitation layer for at least 10 seconds, and then taking out the metal container; or, the extracting solution layer within 1 cm below the liquid level of the non-precipitation layer flows through the surface of the metal container to which the oil film is attached for later use; leaching the oil film of the metal container with ethanol for at least two times, and collecting leacheate; and after ethanol in the leacheate is volatilized, identifying to obtain the farmland soil micro-plastic. The micro-plastic in the farmland soil can be simply, quickly, efficiently and accurately extracted, and the micro-plastic can be conveniently observed and identified by using a microscope and an infrared spectrum. The invention further discloses extraction equipment for the farmland soil micro-plastic, human intervention is reduced, and the effective extraction equipment is provided for simply, quickly, efficiently and accurately extracting the micro-plastic in the farmland soil.

The invention relates to a technical method for rapid asexual propagation and anti-enhancement of hybrid bollgard F1, which comprises the main steps of: firstly, identifying perennial cotton germplasm resources capable of naturally overwintering, and resisting fusarium wilt resistance and greensickness; adopting the perennial cotton germplasm resources as rootstock, scioning and grafting with the hybrid bollgard F1, and cultivating perennial root to obtain grafted plants; and secondly, carrying out large-scale propagation of hybrid bollgard F1 seedlings by using the method, and directly using the hybrid bollgard F1 seeldings for agricultural production. According to the technical method for rapid asexual propagation and anti-enhancement of the hybrid bollgard F1, field cotton hybrid seed production is free, the seed production process of the hybrid bollgard F1 is simplified, the seed purity of the hybrid bollgard F1 is improved, and the disease resistance of the hybrid bollgard F1 is enhanced. The method can be used for large-scale propagation of hybrid bollgard F1 seedlings with obviously improved anti-enhancement property, and is applied to agricultural production.

The invention discloses a sterilizing medicinal ultrasonic coupling agent and a preparation method thereof. The sterilizing medicinal ultrasonic coupling agent contains 0.05 to 2 percent of 2,4,4'-trichloro-2'-hydroxydiphenyl ether, 0.1 to 2 percent of R-polysaccharide, 2 to 7 percent of propylene glycol, 2 to 7 percent of glycerol, 0.6 to 1.5 percent of cross-linking polyacrylic resin and 80.5 to 95.25 percent of distilled water; and in addition, triethanolamine which accounts for 0.8 to 1.2 percent of the total weight of the previous mixture is added into the mixture. The preparation method comprises the following steps of: preparing mixed solution of the propylene glycol and the glycerol; fully dissolving the 2,4,4'-trichloro-2'-hydroxydiphenyl ether and the R-polysaccharide into the prepared mixed solution; preparing cross-linking polyacrylic resin solution; adding the prepared mixed solution into the cross-linking polyacrylic resin solution to form a gelatinous macromolecular substance; and dripping triethanolamine solution into the gelatinous macromolecular substance to obtain neutral ultrasonic coupling agent. The sterilizing medicinal ultrasonic coupling agent thoroughly solves the problem that effective sterilization cannot be realized in short time, and is suitable for sterilizing the skin and the mucosa.

The invention discloses a medical disinfecting and sterilizing ultrasonic coupling agent and a preparation method thereof. The ultrasonic coupling agent comprises 0.1 to 2 percent of R-polysaccharide, 2 to 7 percent of propylene glycol, 2 to 7 percent of glycerol, 0.6 to 1.5 percent of crosslinking (polyacrylic acid) resin, and 82.8 to 95.3 percent of distilled water; and in addition, triethanolamine is added in an amount which is 0.8 to 1.2 percent based on the total weight of the above materials. The preparation method comprises the following steps: preparing mixed solution of propylene glycol and glycerol; completely dissolving the R-polysaccharide in the prepared mixed solution; preparing solution of crosslinking (polyacrylic acid) resin; adding the prepared mixed solution containing the R-polysaccharide into the solution of crosslinking (polyacrylic acid) resin to obtain a gelatinous polymer, supplementing distilled water and stirring the solution uniformly; and slowly dripping solution of triethanolamine to neutralize the obtained ultrasonic coupling agent. In the invention, the problem that effective disinfection cannot be realized in a short time is solved and the preparation process is simple.

The invention discloses a beautifying ultrasonic coupling agent and a preparation method and application thereof, and belongs to the technical field of ultrasonic beautifying. The beautifying ultrasonic coupling agent is prepared from 0.4-2% of gel, 5-10% of a moisturizing agent, 5-15% of beautifying plant essence, 0.4-0.8% of an antioxidant and 72.2-89.2% of water. The beautifying ultrasonic coupling agent selects various natural plant formulas, an ultrasonic probe can be isolated from skin, ultrasonic energy is transmitted into the deep skin, and cell regeneration is stimulated; meanwhile, the beautifying ultrasonic coupling agent has rich beautifying plant essence components which are guided into the skin through the ultrasonic energy, the exponentially improved beautifying effect is generated under the mutual effect of the ultrasonic energy and the beautifying plant essence, and the problems that the beautifying effect obtained when ultrasonic wave is independently used for beautifying or only make-up appliances are used for beautifying is not ideal are solved.

The invention relates to a method for improving quality of cotton fibers by overexpressing GhCAD6 gene, belonging to the technical field of biology. The method comprises the following steps: constructing a plant overexpression vector pCAMINBIA-2300-GhCAD6 from styrone dehydrogenase gene GhCAD6 in cotton fibers, transforming the vector into agrobacterium LB4404 by agrobacterium mediation, and integrating into cotton to obtain the trans-styrone dehydrogenase gene cotton plant. The field and molecular detection analysis detects that the lignin content in cotton fibers obviously increases in different development periods, the fibrocyte wall thickness obviously decreases in the cotton secondary wall rapid deposition stage, and the ripe cotton fiber surface becomes more compact and smooth. The method can maximally enhance the length of the cotton fibers by 16.935%, enhance the specific strength by 25.10% and enhance the fiber yield by 3.80%, thereby greatly improving the quality of the cotton fibers.

The invention discloses a triaxial free rock-soil dilatometer. A telescopic petal mold cylinder is arranged in the middle of a pressure chamber, the outer side of a sample is wrapped with a rubber membrane and connected into the telescopic petal mold cylinder in a sleeved mode, a bottom plate and a bottom permeable stone are sequentially stacked on a base, the bottom of the sample is supported onthe bottom permeable stone, and a top permeable stone and a top plate are sequentially pressed on the top of the sample; the top cover is provided with a top cover valve and connected with a piston through a piston spring, the bottom of the piston is in contact connection with the top plate, the top dial indicator is connected to the top displacement rod in a pressed mode through a top dial indicator support, and the upper portion and the lower portion of the telescopic petal mold barrel are symmetrically sleeved with displacement rings.The bottom of the petal mold cylinder is slidably connected to the base. A bottom plate water passing valve, a top plate water passing valve and a base water passing valve are arranged on the side face of the base and communicated with the bottom plate, thetop plate and the water tank respectively. The triaxial free rock-soil dilatometer is simple in structure, safe and stable, can well protect the sample when the sample is soaked in water and saturated through the telescopic petal mold cylinder, can flexibly adapt to expansion deformation of the sample, and is high in detection efficiency and accuracy.

The invention provides a lymphatic endothelial cell culture medium. The culture medium comprises a basic culture medium and an additive, and the additive comprises an insulin growth factor, a basic fibroblast growth factor, an epidermal growth factor, a vascular endothelial growth factor, vitamin C, transferrin, bovine serum albumin, cortisol, penicillin and streptomycin. The cell culture medium provided by the invention effectively promotes proliferation of lymphatic endothelial cells. According to the invention, the growth factors and the components are matched and added, properties of primary lymphatic endothelial cells can be effectively maintained, and after expanding for eight generations in vitro, the cells can still maintain original properties to a large extent, so that the survival time is prolonged. The components added in the culture medium are easy to obtain, preparation is convenient, and cost for culturing endothelial cells is reduced.

The invention discloses a method for extracting laminarin from kelp. The method comprises the steps as follows: raw material treatment, ultrasonic treatment, enzymolysis, decolorization, deproteinization and drying. During raw material treatment, enzymolysis is performed after ultrasonic treatment, the characters of proteoglycan protein can be well kept while raw material gelling and denaturationof proteoglycan protein are caused by long-term high-temperature heating treatment in the prior art, and the method can effectively improve the laminarin extraction efficiency as compared with the prior art.

The invention discloses an in vitro preservation culture medium for cassava embryogenic callus. A formula of the in vitro preservation culture medium is that an improved GD culture medium is added with 0.6-0.8mg / L of 2,4-D, 6-7mg / L of LTIBA, 20-25g / L of saccharose and 6.0-6.5g / L of agar. The in vitro preservation culture medium is placed under conditions with a temperature of 13-17 DEG C, illumination of 100-1200Lx and illumination time of 16-18h / d to perform culturing. The culture medium is adopted to carry out in vitro preservation on the cassava embryogenic callus, has embryogenic callus multiplication amount which is 1.0-1.2 times that of a traditional culture medium, cell activity which is 2-4 times that of the traditional culture medium and the best preserving effect when subculture time is prolonged to 120d. The method is adopted to carry out in vitro preservation for cassava embryogenic callus, so that basis is provided for storing cassava germplasm resources.

The invention discloses a high-efficiency preparation method of human skin tissue pluripotent stem cells. The method comprises the following steps: preparation of coating bottle: putting a coating bottle in a 4-DEG C refrigerator, and storing for later use; and preparation of skin stem cells: taking a cheek or chin skin tissue, removing the fat tissue, putting the tissue block in a sterile plate, digesting over night, digesting corium layer small blocks, filtering the cell suspension by a filter, keeping the adherent cells, culturing in an incubator, repeatedly beating upon the bottle bottom by using a suction tube until the cells shed, carrying out bottle subculture on the cells according to the ratio of 1:3, and continuing culturing by 2-5 generations. The method has the advantages of high cell yield, high differentiation efficiency and the like.

The invention discloses a method for cutting seedling-raising of Alnus trabeculosa. The method is characterized by comprising the following steps: (1), collection and treatment of a stalk for cutting; (2), arrangement of a cutting bed; and (3), cutting on the cutting bed. The cutting method provided by the invention has the advantages that the rooting rate of Alnus trabeculosa is high, original properties of plants can be retained, propagation of Alnus trabeculosa is accelerated, and the market demand for garden greening is satisfied.

The invention discloses a preparation method of a yogurt starter culture, and belongs to the technical field of foods. According to the invention, fruit juice is added, and various fruits contain components which are beneficial to the human bodies so as to further increase the efficacy of yogurt. Meanwhile, various sugars are added into a nutrient solution, and a sugar content is high so as to notonly provide nutrient fermentation with a better nutrient environment and improve the activity of strains, but also accelerate a fermentation rate. Besides, the two strains adopted by the invention promote the growth of all parties. Meanwhile, defatted milk powder and an antioxidant are added to form a carrier protective agent, so that the carrier protective agent can enter the interior of cellsand prevent freezing injuries inside and outside the cells. A mixture is subjected to pre-freezing, and gets into a solid state so as to avoid the inactivation of active macromolecules in a process ofvacuum freeze-drying and maintain the original characteristics of the strains. The method solves problems of some existing yogurt starter cultures that a flavor is not heavy enough and sourness is too strong, which result in a poor mouth feel and the need of adding sweeteners.

The invention discloses a sterilizing medicinal ultrasonic coupling agent and a preparation method thereof. The sterilizing medicinal ultrasonic coupling agent contains 0.05 to 2 percent of 2,4,4'-trichloro-2'-hydroxydiphenyl ether, 0.1 to 2 percent of R-polysaccharide, 2 to 7 percent of propylene glycol, 2 to 7 percent of glycerol, 0.6 to 1.5 percent of cross-linking polyacrylic resin and 80.5 to 95.25 percent of distilled water; and in addition, triethanolamine which accounts for 0.8 to 1.2 percent of the total weight of the previous mixture is added into the mixture. The preparation method comprises the following steps of: preparing mixed solution of the propylene glycol and the glycerol; fully dissolving the 2,4,4'-trichloro-2'-hydroxydiphenyl ether and the R-polysaccharide into the prepared mixed solution; preparing cross-linking polyacrylic resin solution; adding the prepared mixed solution into the cross-linking polyacrylic resin solution to form a gelatinous macromolecular substance; and dripping triethanolamine solution into the gelatinous macromolecular substance to obtain neutral ultrasonic coupling agent. The sterilizing medicinal ultrasonic coupling agent thoroughly solves the problem that effective sterilization cannot be realized in short time, and is suitable for sterilizing the skin and the mucosa.

The invention discloses an adherent cell scanning electron microscope carrier which comprises a scanning electron microscope sample support (1). A base plate (3) of a tissue culture (TC) surface cell culture dish is fixed on the scanning electron microscope sample support (1) through an electric conduction adhesive layer (2), a cell layer (4) is coated on the base plate (3) of the TC surface cell culture dish, a conductive film (5) is arranged on the cell layer (4), and a conductive strip (6) is connected with the conductive film (5) and the electric conductive adhesive layer (2) in a crossed mode. According to the preparation method of the adherent cell scanning electron microscope carrier directly cultures cells by means of the TC surface cell culture dish, ethanol is replaced with tert butyl alcohol to be used as dehydrating agent, the adherent cell scanning electron microscope carrier is prepared by means of the base plate of the TC surface cell culture dish which is coated with the cell layer, and therefore, the adherent cell scanning electron microscope carrier and the preparation method have the advantages of being simple, rapid, convenient to use and the like.