Lymphatic endothelial cell culture medium, and preparation method and application thereof

A technology for endothelial cells and culture medium, which is applied in the direction of cell culture active agents, biochemical equipment and methods, animal cells, etc., can solve problems such as high price, and achieve the effects of easy acquisition, promotion of proliferation, and prolongation of survival time.

Pending Publication Date: 2020-03-27
NANTONG UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] At present, the formulations of endothelial medium sold in the market are confidential and

Method used

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  • Lymphatic endothelial cell culture medium, and preparation method and application thereof
  • Lymphatic endothelial cell culture medium, and preparation method and application thereof

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Embodiment Construction

[0031] In order to make the technical problems, technical solutions, and advantages to be solved by the present invention clearer, the following will describe in detail with reference to specific embodiments.

[0032] The present invention provides a lymphatic endothelial cell culture medium, which includes a basic medium and additives. The additives include insulin growth factor, basic fibroblast growth factor, epidermal growth factor, vascular endothelial growth factor, vitamin C, and Ferritin, bovine serum albumin, cortisol, penicillin and streptomycin.

[0033] The lymphatic endothelial cell culture medium adopts the following formula:

[0034] Fetal bovine serum 50ml;

[0035] Insulin growth factor 20μg;

[0036] Basic fibroblast growth factor 10μg;

[0037] Epidermal growth factor 5μg;

[0038] Vascular endothelial growth factor 0.5μg;

[0039] Vitamin C 1mg;

[0040] Transferrin 5g;

[0041] Bovine serum albumin 10g;

[0042] Cortisol 0.2mg;

[0043] Penicillin 1×10 5 U;

[0044] Strept...

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Abstract

The invention provides a lymphatic endothelial cell culture medium. The culture medium comprises a basic culture medium and an additive, and the additive comprises an insulin growth factor, a basic fibroblast growth factor, an epidermal growth factor, a vascular endothelial growth factor, vitamin C, transferrin, bovine serum albumin, cortisol, penicillin and streptomycin. The cell culture medium provided by the invention effectively promotes proliferation of lymphatic endothelial cells. According to the invention, the growth factors and the components are matched and added, properties of primary lymphatic endothelial cells can be effectively maintained, and after expanding for eight generations in vitro, the cells can still maintain original properties to a large extent, so that the survival time is prolonged. The components added in the culture medium are easy to obtain, preparation is convenient, and cost for culturing endothelial cells is reduced.

Description

Technical field [0001] The invention belongs to the technical field of cell culture, and specifically relates to a lymphatic endothelial cell culture medium and a preparation method and application thereof. Background technique [0002] Lymphatic endothelial cells (LECs) are a single layer of flat epithelium lining the inner surface of lymphatic vessels, which have the functions of selective barrier, hemostasis, anticoagulation and transportation. LECs not only participate in physiological processes such as maintaining body fluid balance, regulating lymphocyte recirculation and immune response, but also closely related to pathological processes such as inflammation and tumor metastasis. [0003] In recent years, the research on LECs has gradually increased, and the in vitro expansion and culture of the cells has become the most basic link in the research and application process. However, in the current study, LECs are all primary cultures, and there is often mixed cells during the...

Claims

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Application Information

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IPC IPC(8): C12N5/078
CPCC12N5/0634C12N2501/105C12N2501/115C12N2501/11C12N2501/165C12N2500/38C12N2500/24C12N2501/998C12N2501/39
Inventor 李杏玉曹晓蕾陆锦标邵晓轶秦婧吴圆圆
Owner NANTONG UNIVERSITY
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