Method for rapid breeding by pinellia ternate stems

A pinellia, rapid technology, applied in the field of traditional Chinese medicine, can solve the problems of unfavorable large-scale production, etc., and achieve the effects of favorable air circulation, fast breeding speed and simple operation

Inactive Publication Date: 2013-12-04
SOUTHWEST UNIVERSITY FOR NATIONALITIES +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The breeding cycle of this breeding method generally takes 7-16 months, and only 1-2 times of breeding can be carried out a year, which is not conducive to large-scale production

Method used

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  • Method for rapid breeding by pinellia ternate stems
  • Method for rapid breeding by pinellia ternate stems
  • Method for rapid breeding by pinellia ternate stems

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Example 1 Breeding by the method of the present invention

[0048] Sterilization and sterilization of the culture container: use an autoclaveable plant tissue culture box (the material is polypropylene, the culture box is a 25 × 25cm square box, and the lid has 1 to 3 openable and closable ventilation holes). The body and the box cover form a box with an overall height of 4.5cm, which can be sterilized by autoclaving.

[0049] Preparation of medium: The medium used in the present invention is a liquid MS induction medium without any auxin, which is shown in Table 1.

[0050] Inoculation: Select strong virus-free seedlings that have been cultured for 30 days. In a sterile workbench, cut off the petioles and pearl buds of the virus-free seedlings, cut the petioles and pearl buds into 3-5 segments, and inoculate them evenly into in the above induction medium. After 2 weeks of culture, the segments were cut into about 5 to 10 small segments. The cut sections were planted...

Embodiment 2

[0053]Embodiment 2 adopts the method of the present invention to carry out breeding

[0054] Sterilization and sterilization of the culture container: A plant tissue culture box that can be sterilized by autoclaving is used. The box is composed of a box body and a box cover.

[0055] Media Preparation: The media was liquid MS induction medium without any auxin, which is shown in Table 1.

[0056] Inoculation: Select strong virus-free seedlings that have been cultured for 40 days. In a sterile workbench, cut off the petioles and pearl buds of the virus-free seedlings, cut the petioles and pearl buds into 3 to 5 segments, and inoculate them evenly. in the above induction medium. After 1.5 weeks of culture, the segments were cut into 5-10 small segments. Small segments were inoculated at a density of 1.0 × 1.0 cm, and 240 segments were placed in each culture box, for a total of 10 boxes.

[0057] Induction culture: The culture box inoculated with avirulent cut seedlings of Pin...

Embodiment 3

[0059] Example 3 Breeding by the method of the present invention

[0060] Sterilization and sterilization of the culture container: A plant tissue culture box that can be sterilized by autoclaving is used. The box is composed of a box body and a box cover.

[0061] Media Preparation: The media was liquid MS induction medium without any auxin, which is shown in Table 1.

[0062] Inoculation: Select strong tissue culture detoxified seedlings with a seedling age of 35 days. In a sterile workbench, cut off the petioles and buds of the detoxified seedlings. Inoculated into the above induction medium. After 1 week of culture, the segments were cut into 5-10 small segments. The cut sections were planted at a density of 1.3 × 1.3 cm, and 230 sections were placed in each culture box, for a total of 10 boxes.

[0063] Induction culture: The culture box inoculated with avirulent cut seedlings of Pinellia sinensis was treated for 5 weeks under a total dark photoperiod of 24 hours a day...

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Abstract

The invention relates to a method for rapid breeding by pinellia ternate stems, and belongs to the field of traditional Chinese medicine resources. The method has a short breeding period. The method comprises the following steps of a, inoculation and b, induced culture. The method utilizes non-medicinal parts of pinellia ternate to carry out tissue culture, has a fast breeding rate and a low production cost, and is suitable for large-scale, fast and normalized breeding of pinellia ternate seeds. The invention also provides a culture box for pinellia ternate breeding. The culture box comprises a box body and a box cover. The box body and the box cover are quadrate. The box cover is provided with an air port. An air port cover is arranged on the air port. The air port is in a threaded connection relationship with the air port cover. The culture box for pinellia ternate breeding can fully utilize a space, is conducive to air circulation and can improve breeding effects.

Description

technical field [0001] The invention relates to a rapid breeding method using Pinellia stems, belonging to the field of traditional Chinese medicine. Background technique [0002] Pinellia is a perennial herb of Araceae, an important Chinese medicinal material, which has the functions of drying dampness and resolving phlegm, and stomach and relieving vomiting. The traditional Pinellia is based on the production of virus-free vaccines in the laboratory, and is completed by transferring in a greenhouse, a net room, and a field culture. The reproductive cycle of this type of reproduction generally takes 7 to 16 months, and only 1 to 2 reproductions can be performed a year, which is not conducive to large-scale production. SUMMARY OF THE INVENTION [0003] The technical problem of the present invention is to provide a method for rapid breeding using Pinellia stems, and the method has a short reproduction cycle. [0004] The technical scheme of the present invention is to uti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 曾锐刘海萍张海先李琼任桂友王爽
Owner SOUTHWEST UNIVERSITY FOR NATIONALITIES
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