The Method of Rapid Breeding by Utilizing Pinellia Stems

A kind of Pinellia, fast technology, applied in the field of traditional Chinese medicine, can solve problems such as unfavorable large-scale production, and achieve the effects of favorable air circulation, fast breeding speed and short cultivation period

Inactive Publication Date: 2016-01-20
SOUTHWEST UNIVERSITY FOR NATIONALITIES +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The breeding cycle of this breeding method generally takes 7-16 months, and only 1-2 times of breeding can be carried out a year, which is not conducive to large-scale production

Method used

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  • The Method of Rapid Breeding by Utilizing Pinellia Stems
  • The Method of Rapid Breeding by Utilizing Pinellia Stems
  • The Method of Rapid Breeding by Utilizing Pinellia Stems

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Embodiment 1 adopts the method of the present invention to carry out breeding

[0048] Disinfection and sterilization of the culture container: use a plant tissue culture box that can be autoclaved (the material is polypropylene, the culture box is a 25×25cm square box, and the box cover has 1 to 3 openable air holes). The body and the lid form a box with an overall height of 4.5cm, which is autoclaved for use.

[0049] Medium preparation: The medium used in the present invention is a liquid MS induction medium without any auxin, see Table 1.

[0050] Inoculation: Select and cultivate strong virus-free seedlings with a seedling age of 30 days, cut off the petioles and bulbils of the virus-free seedlings in a sterile workbench, cut the petioles and bulbils into 3 to 5 segments, and inoculate them evenly on the in the above-mentioned induction medium. After 2 weeks of culture, callus tissue grew on the segment, and the segment was cut into about 5-10 small segments. Th...

Embodiment 2

[0053]Embodiment 2 adopts the method of the present invention to carry out breeding

[0054] Disinfection and sterilization of the culture container: a plant tissue culture box that can be autoclaved is used. The box is composed of a box body and a box cover. The overall height is 4.5cm, and the length and width are 25cm.

[0055] Medium preparation: the medium is a liquid MS induction medium without any auxin, see Table 1.

[0056] Inoculation: Select and cultivate strong virus-free seedlings with a seedling age of 40 days, cut off the petioles and bulbils of the virus-free seedlings in a sterile workbench, cut the petioles and bulbils into 3 to 5 segments, and inoculate them evenly on the In the induction medium mentioned above. After culturing for 1.5 weeks, callus tissue grew on the segment, and the segment was cut into 5-10 small segments. Small segments were inoculated at a density of 1.0×1.0 cm, and 240 segments were placed in each culture box, 10 boxes in total.

[...

Embodiment 3

[0059] Embodiment 3 adopts the method of the present invention to carry out breeding

[0060] Disinfection and sterilization of the culture container: a plant tissue culture box that can be autoclaved is used. The box is composed of a box body and a box cover. The overall height is 4.5cm, and the length and width are 25cm.

[0061] Medium preparation: the medium is a liquid MS induction medium without any auxin, see Table 1.

[0062] Inoculation: Select strong tissue-cultured virus-free seedlings with a seedling age of 35 days, cut off the petioles and bulbils of the virus-free seedlings in a sterile workbench, cut the petioles and bulbils into 3 to 5 segments, and divide them evenly. Inoculated into the above induction medium. After culturing for 1 week, callus tissue grew on the segment, and the segment was cut into 5-10 small segments. The cut sections were planted at a density of 1.3×1.3cm, and 230 sections were placed in each culture box, 10 boxes in total.

[0063] In...

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Abstract

The invention relates to a method for rapid breeding by pinellia ternate stems, and belongs to the field of traditional Chinese medicine resources. The method has a short breeding period. The method comprises the following steps of a, inoculation and b, induced culture. The method utilizes non-medicinal parts of pinellia ternate to carry out tissue culture, has a fast breeding rate and a low production cost, and is suitable for large-scale, fast and normalized breeding of pinellia ternate seeds. The invention also provides a culture box for pinellia ternate breeding. The culture box comprises a box body and a box cover. The box body and the box cover are quadrate. The box cover is provided with an air port. An air port cover is arranged on the air port. The air port is in a threaded connection relationship with the air port cover. The culture box for pinellia ternate breeding can fully utilize a space, is conducive to air circulation and can improve breeding effects.

Description

technical field [0001] The invention relates to a method for rapid breeding by using pinellia stems, belonging to the field of traditional Chinese medicines. Background technique [0002] Pinellia is a perennial herbaceous plant of Araceae. It is an important traditional Chinese medicinal material, which has the functions of drying dampness, resolving phlegm, harmonizing stomach and relieving vomiting. The traditional pinellia is based on the production of virus-free seedlings in the laboratory, and is completed through transfer to greenhouses, net rooms, and field cultivation. The breeding cycle of this breeding method generally takes 7-16 months, and only 1-2 times of breeding can be carried out a year, which is not conducive to large-scale production. Contents of the invention [0003] The technical problem of the present invention is to provide a method for rapid breeding using pinellia stems, which has a short breeding cycle. [0004] Technical scheme of the present...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 曾锐刘海萍张海先李琼任桂友王爽
Owner SOUTHWEST UNIVERSITY FOR NATIONALITIES
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