Tissue culture method for Clematis Multi Blue

A tissue culture, clematis technology, applied in the field of plant tissue culture, can solve problems such as backward propagation technology

Inactive Publication Date: 2006-11-29
NANJING FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] The purpose of the present invention is to provide a kind of adopting tissue culture method to improve its reproduction coefficient to adapt to the needs of introduction and popularization for the problem that the existing reproduction technology of clematis (Clematis Multi-Blue) is relatively backward.

Method used

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Examples

Experimental program
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Embodiment Construction

[0044] 1. The implementation mode of cluster bud propagation

[0045] 1.1 Take the stem tip of Clematis Multi-Blue and disinfect it with 70% alcohol on the ultra-clean workbench, rinse it with sterile water, then disinfect it with 0.1% mercury liter, rinse it with sterile water and set it aside;

[0046] 1.2 Primary culture: inoculate the sterilized explants into MS+BA0.5mg·L -1 +NAA0.01mg·L -1 , subcultured after 30 days;

[0047] 1.3 Subculture: Transfer the explants from the primary culture to 1 / 2MS+TDZ0.5mg·L -1 +NAA0.01mg·L -1 Subculture was carried out on the culture medium, and cultured for 30 days;

[0048] 1.4 Rooting culture: cut 3.5cm-long clustered shoots and insert them into the rooting medium: 1 / 2MS+NAA0.3mg·L -1 , after 1 week of dark culture, culture under light, light intensity 2000lx, root after 2 weeks.

[0049] 2. Implementation of embryoid body reproduction

[0050] 2.1 Take the stem tip of Clematis Multi-Blue and disinfect it with 70% alcohol on th...

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Abstract

A tissue culture method for clematis Multi-Blue includes such steps as disinfecting the stem tip of clematis multi-blue by alcohol or corrosive sublimate, flushing with aseptic water, primary culture, secondary culture, rooting culture and reproducing embryoids.

Description

1. Technical field [0001] The invention relates to a plant tissue culture method, in particular to a Clematis Clematis Multi-Blue tissue culture method. 2. Background technology [0002] Clematis belongs to Ranunculaceae Ranunculaceae Clematis L. (Wang Wen-Tsai.2000, Notes on the genus Clematis (Ranunculaceae) (I). Acta Phytotaxonomica Sinica.38 (4): 305-336; Wang Wen -Tsai. 2000, Notes on the genus Clematis (Ranunculaceae) (II). Acta Phytotaxonomica Sinica. 38 (5): 401-429; .38(6):497~514; Wang Wen-Tsai.2001, Notes on thegenus Clematis (Ranunculaceae)(IV). Acta Phytotaxonomica Sinica., 39(1):1-19; Wang W-T.2004.A revision of Clematis sect. Aspidanthera s. 1. (Ranunculaceae). Acta. Phytotaxonomica Sinica 42: 1-72, 97-135). It is a kind of vine with high ornamental value and a variety of stress resistance. It has high ornamental value (Brandenburg WA. 2000. Meclatis in Clematis: Yellow Flowering Clematis Species. Systematic Studies in Clematis I. (Ranunculaceae), Inclusive ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00C12N5/04
Inventor 方炎明张启香
Owner NANJING FORESTRY UNIV
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