Method for tissue culture of witch hazel

A tissue culture and witch hazel technology, applied in the field of plant tissue culture, can solve problems such as outdated reproductive technology of witch hazel

Inactive Publication Date: 2012-04-25
NANJING FORESTRY UNIV
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

[0009] The purpose of the present invention is to provide a tissue culture method for Hamamelis hamamelis in view of the relatively outdated problem of the existing propagation technology of Hamamelis hamamelis.

Method used

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Embodiment Construction

[0041] A kind of witch hazel tissue culture method, its culture step is as follows:

[0042] (1) Disinfection treatment of explants

[0043] Select the robust seeds of a single Hamamelis hamamelis plant that matured in the same year, rinse them with tap water for 2 hours, sterilize them with 70% ethanol for 30 seconds on an ultra-clean workbench, rinse them with sterile water for 3 to 4 times, and then dilute them 10 times with a concentration of 0.525 Sterilize with %NaClO for 20 minutes and vacuumize, rinse with sterile water for 5 times, blot dry the water on the seed surface with sterilized filter paper, and peel off the lignified outer testa to be inserted into MS or 1 / 2MS basic medium, sucrose 20 ~30g·L -1 , agar 0.7%, pH 5.7, culture temperature 25°C;

[0044] (2) Aseptic germination of seeds

[0045] The peeled mature embryos were inserted into the MS basic medium, and germinated after dark culture for 2 weeks;

[0046] ① Hardening and transplanting of sterile seed...

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Abstract

The invention discloses a method for tissue culture of witch hazel. The method comprises the following steps of: disinfection of an explant, which is to (1) disinfect seeds of the witch hazel by alcohol and sodium hypochlorite respectively and washing the seeds of the witch hazel by sterilized water to strip episperm of the seeds of the witch hazel; aseptic germination of the seeds(2); adventitious bud propagation culture(3); inductive culture(4); propagation culture(5); strong seedling culture(6); rooting culture(7) and hardening seedling and transplantation(8). By adopting the tissue culture method, the seedling period of the seed of the witch hazel is greatly shortened. Compared with the cottage propagation, the method of the invention can effectively improve the propagation coefficient of the witch hazel. In a growth cycle, the propagation coefficient of the tissue culture method of the invention is 50 to 60 times that of the cottage propagation.

Description

1. Technical field [0001] The invention relates to a plant tissue culture method, in particular to a tissue culture method of witch hazel. 2. Background technology [0002] Hamamelis mollis (Hamaelis mollis Oliv.) is a small tree or shrub belonging to the genus Hamamelis of the family Hamamelis, which is the only species in my country (Flora of China, 1979, Vol. 35, Part II, 73). It is native to the eastern, central and southwestern parts of my country, and is mainly distributed in the mountainous areas of Zhejiang, Fujian, Anhui and other provinces, mostly scattered. Witch hazel is a positive flower and tree, which likes warm and humid climate and is relatively cold-tolerant. [0003] As a flower and tree with ornamental value, witch hazel has an elegant tree shape, with a spherical or oval crown. The flowering period is early spring and February, flowers first and then leaves. The flower shape is peculiar, the fragrance is pleasant, and the leaves turn yellow to red in a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00A01G31/00
Inventor 方炎明张启香
Owner NANJING FORESTRY UNIV
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