Technical method for rapid asexual propagation and anti-enhancement of hybrid bollgard F1

A technology of insect-resistant cotton and technology is applied in the field of promotion and application of hybrid insect-resistant cotton F1, which can solve the problems of low seed production efficiency, unstable experiment, complicated process, etc., achieve a feasible variety promotion model, simplify production procedures, and maintain original The effect of traits

Inactive Publication Date: 2012-06-20
HUBEI PROVINCIAL SEED GRP CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] (2) Disorderly competition in seed production bases makes it difficult for enterprises to guarantee the management and technical level of the bases, which directly affects the quality of cotton seeds
[0005] (3) Since manual detasseling and pollination requires a large amount of concentrated labor, under the current situation of few agricultural workers, high wages, and relatively low efficiency in seed production, the production of hybrid cotton seeds is stuck in a bottleneck, and it is difficult to expand the scale, and it is difficult to guarantee the quality of the seeds
[0007] (1) Cotton tissue culture is limited by the genotype of embryogenesis and plant regeneration;
[0008] (2) The cultivation cycle is long and the process is complicated, mostly at the level of obtaining a small number of plants through indirect, long-term and complicated multiple subcultures;
[0009] (3) The experiment is unstable and the repeatability is poor. low regeneration rate;
[0010] (4) The deformity of cotton seedlings obtained from cotton tissue culture is very serious;
[0011] (5) Lack of a complete cotton tissue culture and efficient plant regeneration system suitable for a wide range of genotypes;
[0012] (6) The transplanting survival rate of regenerated plants is low

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] In 2008, Eza cotton 13F1 and sea-island cotton MD1010 were sown in Nanning, Guangxi. Sea-island cotton MD1010 is a perennial sea-island cotton with a tall plant of about 130-135 cm. Leaves heart-shaped, 5-lobed, deeply lobed. The flowers are light yellow, the anthers are densely packed on the short filaments of the stamen tube, and the stigma is long. The bell is elongated, pointed, three-chambered, and rarely four-chambered. Resistance to blight and Verticillium wilt, basically no disease in naturally severely diseased fields. During the cultivation period, sea-island cotton MD1010 was used as the rootstock, and Eza cotton 13F1 was used as the scion for grafting. After grafting, it was planted and managed according to the local annual cotton cultivation and management methods, and non-F1 grafted cotton seedlings were removed in the year of grafting.

[0029] From 2009 to March 2011, sow the rootstock sea-island cotton MD1010 in the nutrition cup. When it grows true l...

Embodiment 2

[0032] In 2009, in Ezhou City, Hubei Province, according to the method in Example 1 above, sea island cotton MD1010 was used as the rootstock, and Cuza 411F1 was propagated by grafting, and non-F1 hybrids were removed in the year of cultivation. In the winter of the same year, the greenhouse was used to keep the heat, so that the average temperature in the greenhouse was greater than or equal to 10°C. Make it survive the winter safely and allow perennial roots to grow. In 2010, grafted with reference to the method in Example 1 to obtain grafted seedlings of Cuza 411F1, which were transplanted to the local area for planting.

[0033] Through observation and experimentation, compared with Tongza 411F1 grafted seedlings, Tongza 411F1 has a tower-shaped plant, slightly compact, with hairy stems, medium-sized bract leaves, light green leaves, oval bells, and bell tips. Blunt and short, growth period 121.7 days, single boll weight 5.7 grams, large sample clothing percentage 40.86%,...

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PUM

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Abstract

The invention relates to a technical method for rapid asexual propagation and anti-enhancement of hybrid bollgard F1, which comprises the main steps of: firstly, identifying perennial cotton germplasm resources capable of naturally overwintering, and resisting fusarium wilt resistance and greensickness; adopting the perennial cotton germplasm resources as rootstock, scioning and grafting with the hybrid bollgard F1, and cultivating perennial root to obtain grafted plants; and secondly, carrying out large-scale propagation of hybrid bollgard F1 seedlings by using the method, and directly using the hybrid bollgard F1 seeldings for agricultural production. According to the technical method for rapid asexual propagation and anti-enhancement of the hybrid bollgard F1, field cotton hybrid seed production is free, the seed production process of the hybrid bollgard F1 is simplified, the seed purity of the hybrid bollgard F1 is improved, and the disease resistance of the hybrid bollgard F1 is enhanced. The method can be used for large-scale propagation of hybrid bollgard F1 seedlings with obviously improved anti-enhancement property, and is applied to agricultural production.

Description

technical field [0001] The invention relates to the field of popularization and application of hybrid insect-resistant cotton F1, in particular to a technical method for asexual rapid propagation and resistance enhancement of hybrid insect-resistant cotton F1. Background technique [0002] At present, the main types of cotton cultivated are conventional cotton and hybrid cotton. 70% of the cotton area in the Yangtze River Basin is planted with F1, an insect-resistant hybrid cotton that is crossed between varieties. The production of hybrid cotton seeds among existing varieties all adopts artificial detasseling, artificial pollination and hybridization to obtain hybrid F1 seeds. Its main disadvantages are: [0003] (1) For the production of hybrid cotton seeds, parental breeding and hybrid seed production must be carried out separately, and impurities and impurities must be removed every year. [0004] (2) Disorderly competition in seed production bases makes it difficult...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/00
Inventor 袁国保耿月明邓岩王世才郭承亮杨新明
Owner HUBEI PROVINCIAL SEED GRP CO LTD
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