Method for preparing frozen sections of horse, donkey and mule testicles

Abstract

The invention discloses a method for preparing frozen sections of horse, donkey and mule testicles. The method comprises the following steps that after blocky testicle tissues are placed in a Bouin fixing solution to be fixed, the fixed testicle tissues are dehydrated in a sucrose solution, and the dehydrated testicle tissues are embedded, and frozen and sliced to obtain the testicle frozen sections. The method is used for preparing the frozen sections of the horse, donkey and mule testicles, and can denature, solidify and precipitate protein, and keep original morphological structures of cells and tissues to prevent autolysis and decay of the tissues, enzymes in the cells are converted into insoluble substances so as to keep the original structures, the tissues are in a certain hardened state after being fixed, the toughness of the tissues is improved, deformation is not easy, the cell integrity is kept, and the good cell tissue morphological structures can be shown after HE stainingand DAPI nuclear staining.

Description

technical field [0001] The invention relates to the technical field of preparation of pathological slices, in particular to a method for preparing frozen slices of testicles of horses, donkeys and mules. Background technique [0002] Frozen section is a commonly used method of rapid slice preparation in clinical pathological examination. It is a technique for making slices after the tissue is rapidly frozen to a certain extent under low temperature conditions. Due to the simple, fast construction and time-consuming It is short and has significant advantages in the preservation of antigens, and is often used in some laboratories to carry out physiological, pathological and molecular studies at the tissue level. [0003] At present, in the process of making frozen sections, it is necessary to be very careful in the operation and control of the samples. If there are knife marks, ice crystals, incomplete sections, inappropriate thickness, and poor fixation, etc., it will often l...

AI Technical Summary

Problems solved by technology

[0003] At present, in the process of making frozen sections, it is necessary to be very careful in the operation and control of the samples. If there are knife marks, ice crystals, incomplete sections, inappropriate thickness, and poor fixation, etc., it will often lead to cell swelling and deformation, which will affect the observation. slice state

In the immunohistochemical staining of scientific research experiments, frozen sections can better preserve the activity of cell antigens, but because the testis of large animals is generally larger than that of model animals such as mice, and the tissue water content is high, it is easy to be affected by ice crystals when making frozen sections. Slicing results; it is not easy to succeed in making frozen section plans using the organs and tissues of model animals such as mice and rats, and making frozen sections of horse testis

The traditional liquid nitrogen method makes slices, because the horse testis is large in volume and high in water content, it is easy to ice crystals when sampling and quick-freezing, and at the same time it is easy to produce sudden changes in volume and cause overall deformation, which will cause deformation of testicular tissue cells, various types of cells cannot be identified, and the shape of the testis cannot be observed and nucleus

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