41results about How to "Prevent autolysis" patented technology

The invention provides a cell preservation liquid which is composed of sodium chloride, ethylene diamine tetraacetic acid disodium, PEG10000, Proclin300, methanol, and an acetic acid-sodium acetate buffer solution. The cell preservation liquid reduces the use cost, really effectively protects the integrity and stationarity of cells, prevents cell dissolution and expansion, is easy to popularize and strong in compatibility, and saves the sample preparation time.

The invention discloses a pathological specimen processing method and belongs to the field of specimen processing technology. According to the processing method, sampled pathological tissues successively undergo fixation, dehydration, transparency, wax impregnation, embedding, slicing, staining and mounting so as to obtain a pathological specimen. The fixation is to fix the pathological tissues by the use of a fixative for 4-15 h. The fixative is composed of atractylol, ethoxylated acetylenediol, chlorogenic acid, polyvinylpyrrolidone, sodium chloride, ethyl acetate and pure water. The mounting is to carry out mounting on the stained pathological tissues by the use of a mixture of polyacrylamide, polyvinylpyrrolidone and xylene. According to the invention, toxicity of reagents used in the invention is obviously reduced, the fixation effect is good, degradation and transparency degree are easy to control. Then, the tissues are not easy to deform, embrittle or harden, and mounting is complete and staining is remarkable.

The invention provides a cell fixation solution, detection kit and method for human respiratory tract pathogen infection flow cytometry detection. The cell fixation solution is a PBS solution containing formaldehyde with the volume fraction of 0.1-1% and methyl alcohol with the volume fraction of 60-80%, and the pH of the cell fixation solution is 7.2-7.4. The kit comprises the cell fixation solution, a cell permeating agent and at least one kind of monoclonal antibodies of a human respiratory tract pathogen to be detected, each kind of monoclonal antibodies of the human respiratory tract pathogen to be detected is marked with fluorescein, and different human respiratory tract pathogens to be detected are provided with different fluoresceins. The detection process is automatic, labor cost is saved, the result is objective and accurate, single-sample multiple detection can be achieved, and the pathogen infection condition can be better reflected.

The invention discloses a preserving stationary liquid for pathological tissues, and belongs to the technical field of sample processing. The preserving stationary liquid for pathological tissues is prepared from 12 to 15g of menthol, 10 to 20g of glycerin, 5 to 10ml of ethoxylation acetylenediol, 10 to 20g of chlorogenic acid, 30 to 70g of polyvinylpyrrolidone, 30 to 40g of sodium chloride, and 1000ml of pure water. The preserving stationary liquid for pathological tissues has the advantages that the formaldehyde solution is completely replaced; the property is stable, any toxic or side effect is avoided, the softness and hardness are proper after the pathological sample is subject to stationary treatment, the obvious shrinkage and hardening are avoided, the toughness is good, and the obvious deformation and discoloring are avoided; in the stationary process, the stationary liquid has no turbid or settling phenomenon.

The invention relates to a direct drinking water purifying method and a direct drinking water purifying device. The direct drinking water purifying method comprises three-stage treatment steps, namely active carbon adsorption, ultra-filtration film filtration and ultraviolet sterilization in turn. The direct drinking water purifying device comprises a shell, and a water inlet and a water outlet which are connected on the shell; and the device also comprises a first-stage carbon adsorption treatment device, a second-stage ultra-filtration film treatment device and a third-stage ultraviolet treatment device. The method and the device can effectively solve the problems of poor trace pollutant removing effect, secondary pipeline pollution and the like in the conventional drinking water treatment process through a technique with low energy consumption and low cost.

The present invention provides a cobalt-based alloy electroless plating solution comprising a cobalt precursor, a tungsten precursor, a phosphorus precursor, a reducing agent, a complexing agent, a pH regulator and a stabilizer, in which the reducing agent is dimethylamine borane (DMAB) or borohydride and the stabilizer is one or more compounds selected from a group consisting of imidazole, thiazole, triazole, disulfide and their derivatives; and an electroless plating method using the cobalt-based alloy electroless plating solution, as well as a thin film prepared by the same. According to the present invention, the cobalt-based alloy electroless plating solution is stable enough for long-term reuse and prevents deterioration of metal thin-film quality by inhibiting the formation of a precipitate. The present invention further provides an electroless plating method using the cobalt-based alloy electroless plating solution, and a cobalt-based alloy thin film prepared by the same.

The invention relates to a method for producing a high-activity lactic acid bacteria agent by a two-step drying method, belonging to the technical field of microorganisms of preservation or activity maintenance. The method comprises steps of lactobacillus mire preparation and vacuum freeze drying. The method is characterized in that: before the step of vacuum freeze drying, the prepared lactobacillus mire is put in a vacuum drying box to carry out vacuum drying for 4-20 hours under a vacuum degree below 500Pa and a vacuum drying temperature of 25-45 DEG C. The survival rate of thalli in the method is higher than that of thalli of a method which directly carries out freeze drying, and the load and the operation time of the vacuum freeze drying can be reduced. The invention has small equipment loss and low energy consumption and can effectively save cost and improve the production efficiency.

The invention discloses a corn-free pig feed which is prepared from the following raw materials in percentage by weight: 68-64% of wheat middling, 2-3% of pichia pastoris, 3-2% of animal hide glue, 2.9-3.8% of cottonseed meal, 5-6% of corn germ meal, 14-16% of bran, 4% of premix, 0.1-0.2% of choline chloride and 1% of a microorganism additive. Water is added into the raw materials, and the raw materials are stacked and fermented for 5-7 days and are fed to pigs directly. According to the corn-free pig feed, wheat middling takes the place of corn completely, the use amount of bean pulp is reduced, the wheat middling is increased, and cheap raw materials of pichia pastoris and animal hide glue with low values are abandoned, so that the resource is saved, the feed cost is lowered, the mycotoxin is reduced, and the productivity of pigs is increased.

The invention discloses a high-purity extraction method of tremella polysaccharide. The method comprises the following steps: S1, taking tremella, washing the tremella with water, airing and cutting the tremella, and carrying out vacuum drying, grinding and superfine vibration; S2, carrying out leaching for 4h in water at 100 DEG C according to the material-water ratio of 1:40, carrying out heating and stirring for 8h in a boiling water bath, carrying out centrifugation for removing residues, carrying out auxiliary filtration on the supernatant fluid with kieselguhr, carrying out water washing, mixing the filtrates, and then, carrying out stirring in a water bath at 80 DEG C to obtain syrup; S3, adjusting the pH value to be 7 by using 2mol/L NaOH, carrying out filtration with a micro-filtration membrane, and carrying out membrane distillation to obtain raw material fluid; and S4, feeding the raw material fluid into an ultra-filtration device by virtue of an increased pump, carrying out cyclic concentration and separation three times by utilizing an ultra-filtration membrane, and carrying out membrane distillation as well as concentration and separation to obtain a concentrated solution. The micro-filtration membrane is adopted for carrying out filtration to obtain the raw material fluid, and then, the ultra-filtration device is used for carrying out concentration to obtain the tremella polysaccharide which is high in purity, suitable for large-scale production and short in production cycle. Compared with the traditional process equipment, the equipment operating cost is low, the production cost can be effectively reduced, and the economic benefits of enterprises can be improved.

The present document describes an electrophoretic tissue clearing chamber comprising an electrophoresis channel, configured to receive a clarification fluid therethrough; a first clarification fluid inlet, in fluid communication with the electrophoresis channel, configured to be connected to a source of the clarification fluid; a tissue sample holder in fluid communication with the electrophoresis channel, configured to receive a tissue sample to be clarified, and pressurize and homogenously apply the clarification fluid onto the tissue sample; a clarification fluid outlet, in fluid communication with the tissue sample holder, for exit of the clarification fluid from the electrophoretic tissue clearing chamber; and first and a second electrode, opposite one another in the electrophoresis channel, for transmission of an electric field therethrough.

The invention relates to a preparation method of a cell wax block based on a non-bloody cell sample and a cell wax block, and relates to the field of biotechnology. The preparation method comprises the following steps: performing first centrifugation onf a non-bloody cell sample for 5-6min at under a condition of 2,900-3,100r/min; collecting athe first precipitate sample and supernate 0-1.5cm away from the first precipitate sample; performing second centrifugationprecipitation to obtain a second precipitate sample; adding stationary liquid into the second precipitate sample, and mixing uniformly; performing third centrifugation to obtain a third precipitate sample; performing dehydration, transparentizing, wax dipping and embedding on the third precipitate sample. The preparation method provided by the invention has high preparation efficiency and is easy and convenient to operate; the prepared cell wax block has the advantages of permanent storage, many cell components, complete cellstructure and clear high-power background; the diagnosis accuracy is effectively improved.

The present invention relates to a device and a method for collecting a sample of colorectal mucocellular layer excreted immediately following the natural act of defaecation from the surface of the anal area of a human subject, and preservation and analysis of the collected sample for detecting diagnostically informative disease biomarkers.

The invention discloses a method for producing a Bacillus subtilis inoculant by liquid-solid biphasic fermentation, comprising the steps of: inoculating an activated Bacillus subtilis strain in a seedculture medium, and performing shaker culture to obtain a seed liquid; inoculating the seed liquid into a liquid fermentation medium deep in a fermentor, and performing fermentation culture to obtaina liquid fermentation product; inoculating the liquid fermentation product into a porous shallow-tray solid fermentation medium, and performing shallow-tray solid fermentation to obtain a fermentation product of Bacillus subtilis; and drying the fermentation product of Bacillus subtilis under a ventilation condition, and then conducting crushing, so as to obtain the Bacillus subtilis inoculant. The invention utilizes an inexpensive carbon and nitrogen source as a medium and combines the medium with a liquid-solid biphasic fermentation process to produce the Bacillus subtilis inoculant, so that the effective viable bacterial count and spore yield are improved, and the raw material and post-treatment cost of the inoculant are reduced.

The invention discloses a liquid-based cell detection test kit for cervical cancer screening and a use method, and the main points of the technical scheme are as follows: the kit comprises a liquid-based cell cleaning solution and a cell staining solution, and the liquid-based cell cleaning solution comprises a cell preserving solution and sucrose; the cell preserving solution is prepared from thefollowing components: paraformaldehyde, a phosphate buffer solution, ascorbic acid, EDTA-Na2.2H2O, N-acetyl-L-cysteine, 4M NaOH, tween-20 and sterile water, the cell staining solution comprises hematoxylin and an EA50 staining solution, and the hematoxylin comprises the following components: aluminum potassium sulfate, hematoxylin, sodium iodate, acetic acid, absolute ethyl alcohol, glycerol andsterile water; the EA50 staining solution comprises the following components: bright green SF, eosin, methanol, phosphotungstic acid, lithium carbonate, glacial acetic acid and sterile water. The liquid-based cell detection test has the advantages of high cell preservation stability, good cell fixation, preservation of complete cell morphology, prevention of mucus interference and cell aggregation, effective red blood cell lysis and the like; the liquid-based cell detection test is low in price, convenient and easy to obtain, and the liquid-based cell preserving solution low in cost and convenient to popularize and apply.

The invention discloses an Antarctic krill canned food and a making method thereof. The Antarctic krill canned food comprises the following raw materials of 90-98.5%(w/w) of Antarctic krill, 1-4.5%(w/w) of edible oil, 0.05-0.5%(w/w) of chicken essence, 0.1-3.0%(w/w) of table salt, 0.05-0.5%(w/w) of ferrous gluconate, 0.1-0.5%(w/w) of citric acid, 0.1-0.5%(w/w) of Baijiu and 0.1-0.5%(w/w) of oystershell powder. The making method comprises the following steps of (1) soaking frozen Antarctic krill in a citric acid solution for unfreezing; (2) putting the Antarctic krill treated with citric acidin sour saline water, performing blanching, fishing out the blanched Antarctic krill, and performing draining for standby application; (3) heating a pot, adding vegetable oil, when oil temperature reaches 100-145 DEG C, adding Baijiu, performing stir-frying for 0.5-3min, adding the Antarctic krill obtained in the step (2), the ferrous gluconate and the oyster shell powder, performing stir-frying for 1-6min, finally adding the chicken essence, and performing uniform stirring; and (4) performing natural cooling, performing packaging, and performing sterilization. The high-quality Antarctic krillcanned food disclosed by the invention maintains palatable taste of the Antarctic krill, nutrient components are good to preserve, the processing technology is succinct, and industrialized productionis facilitated.

The invention discloses a tissue cell immobilizing solution as well as a preparation method and an application method thereof. The tissue cell immobilizing solution comprises the following componentsin parts by weight: 28-36 parts of formaldehyde, 1-2 parts of ethylene glycol, 1-4 parts of sorbitol, 2-3 parts of isobutanol, 7-9 parts of higher alcohol, 2.5-3.6 parts of NaH2PO4.2H2O, 8.2-9.3 partsof Na2HPO4.12H2O and 400-500 parts of H2O. The preparation method of the tissue cell immobilizing solution comprises the following steps: mixing 2.5-3.6 parts of NaH2PO4.2H2O, 8.2-9.3 parts of Na2HPO4.12H2O and 400-500 parts of H2O to form a solution a; mixing 3/4 of the total amount of the solution a with 28-36 parts of formaldehyde to obtain a solution b; mixing a quarter of the total amount ofthe solution a with 1-2 parts of ethylene glycol, 1-4 parts of sorbitol, 2-3 parts of isobutanol and 7-9 parts of higher alcohol to form a solution c; and uniformly mixing the solution b and the solution c to obtain the tissue cell immobilizing solution. The tissue cell immobilizing solution can well maintain the inherent form of tissue cells, and is more environment-friendly.