High-purity extraction method of tremella polysaccharide

Abstract

The invention discloses a high-purity extraction method of tremella polysaccharide. The method comprises the following steps: S1, taking tremella, washing the tremella with water, airing and cutting the tremella, and carrying out vacuum drying, grinding and superfine vibration; S2, carrying out leaching for 4h in water at 100 DEG C according to the material-water ratio of 1:40, carrying out heating and stirring for 8h in a boiling water bath, carrying out centrifugation for removing residues, carrying out auxiliary filtration on the supernatant fluid with kieselguhr, carrying out water washing, mixing the filtrates, and then, carrying out stirring in a water bath at 80 DEG C to obtain syrup; S3, adjusting the pH value to be 7 by using 2mol/L NaOH, carrying out filtration with a micro-filtration membrane, and carrying out membrane distillation to obtain raw material fluid; and S4, feeding the raw material fluid into an ultra-filtration device by virtue of an increased pump, carrying out cyclic concentration and separation three times by utilizing an ultra-filtration membrane, and carrying out membrane distillation as well as concentration and separation to obtain a concentrated solution. The micro-filtration membrane is adopted for carrying out filtration to obtain the raw material fluid, and then, the ultra-filtration device is used for carrying out concentration to obtain the tremella polysaccharide which is high in purity, suitable for large-scale production and short in production cycle. Compared with the traditional process equipment, the equipment operating cost is low, the production cost can be effectively reduced, and the economic benefits of enterprises can be improved.

Description

technical field [0001] The invention relates to the field of tremella polysaccharide extraction, in particular to a high-purity extraction method of tremella polysaccharide. Background technique [0002] Tremella is rich in tremella polysaccharides (accounting for about 60%-70% of the dry weight of tremella). The acidic polysaccharides contained in tremella have a wide range of physiological activities, can improve the body's immunity, anti-tumor, can scavenge free radicals, and induce the body to produce antibodies And interferon, can treat high blood pressure, hyperlipidemia, diabetes and other medical intractable diseases. [0003] The commonly used tremella polysaccharide extraction methods in China include hot water extraction, acid-base extraction and enzymatic extraction. At present, the extraction methods of tremella polysaccharides are mostly hot water extraction or acid-base extraction, but hot water extraction is time-consuming. It is too long, and the yield is...

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Problems solved by technology

[0003] The commonly used tremella polysaccharide extraction methods in China include hot water extraction, acid-base extraction and enzymatic extraction. At present, the extraction methods of tremella polysaccharides are mostly hot water extraction or acid-base extraction, but hot water extraction is time-consuming. It is too long, and the yield is relatively low, time-consuming and laborious, so it is not suitable for large-scale industrial production. Although the acid-base extraction method has a short extraction time, it will destroy the biological activity of tremella polysaccharides, making the extracted tremella polysaccharides medicinal The effect is greatly reduced. At present, the traditional method of extracting tremella polysaccharides has low purity of tremella polysaccharides