Preserving stationary liquid for pathological tissues

A fixative and tissue technology, applied in the field of specimen processing, can solve the problems that the composition of the processing liquid is not easy to control, and its properties are greatly affected by human and environmental factors, so as to prevent tissue and cell autolysis, have no toxic side effects, and have good toughness Effect

Inactive Publication Date: 2017-05-24
NANYANG MEDICAL COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The specimen preservation treatment solution is prepared from pure traditional Chinese medicine. Due to the complexity of the components of the traditional Chines

Method used

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  • Preserving stationary liquid for pathological tissues
  • Preserving stationary liquid for pathological tissues

Examples

Experimental program
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Effect test

Embodiment 1

[0018] A pathological tissue preservation solution, which is composed of: menthol 13g, glycerin 14g, ethoxylated acetylene glycol 7ml, chlorogenic acid 13g, polyvinylpyrrolidone 40g, sodium chloride 32g, pure water to 1000ml.

[0019] The preparation method of the preservation fixative of the above-mentioned pathological tissues is as follows: take respectively menthol, glycerin, ethoxylated acetylene glycol, chlorogenic acid, polyvinylpyrrolidone and sodium chloride of the above-mentioned weight; Completely dissolve pure water to obtain sodium chloride solution; then add glycerin, menthol, chlorogenic acid and ethoxylated acetylenediol in sequence, and stir and mix for 12 minutes; finally add polyvinylpyrrolidone, and add pure water while stirring When the volume of the mixture is 1000ml, continue to stir at 2000rpm / min until the state of the mixture is uniform.

[0020] In order to understand the application of the pathological tissue preservation fixative of the present inv...

Embodiment 2

[0022] A pathological tissue preservation solution consists of: menthol 14g, glycerin 16g, ethoxylated acetylene glycol 8ml, chlorogenic acid 15g, polyvinylpyrrolidone 50g, sodium chloride 35g, pure water to 1000ml.

[0023] The preparation method of the preservation fixative of the above-mentioned pathological tissues is as follows: take respectively menthol, glycerin, ethoxylated acetylene glycol, chlorogenic acid, polyvinylpyrrolidone and sodium chloride of the above-mentioned weight; Completely dissolve pure water to obtain sodium chloride solution; then add glycerin, menthol, chlorogenic acid and ethoxylated acetylenediol in sequence, stir and mix for 15 minutes; finally add polyvinylpyrrolidone, and add pure water while stirring When the volume of the mixture is 1000ml, continue to stir at 2500rpm / min until the state of the mixture is uniform.

Embodiment 3

[0025] A pathological tissue preservation solution consists of: menthol 12g, glycerin 10g, ethoxylated acetylene glycol 5ml, chlorogenic acid 10g, polyvinylpyrrolidone 30g, sodium chloride 30g, pure water to 1000ml.

[0026] The preparation method of the preservation fixative of the above-mentioned pathological tissues is as follows: take respectively menthol, glycerin, ethoxylated acetylene glycol, chlorogenic acid, polyvinylpyrrolidone and sodium chloride of the above-mentioned weight; Completely dissolve pure water to obtain sodium chloride solution; then add glycerin, menthol, chlorogenic acid and ethoxylated acetylenediol in sequence, and stir and mix for 10 minutes; finally add polyvinylpyrrolidone, and add pure water while stirring When the volume of the mixture is 1000ml, continue stirring at 3000rpm / min until the state of the mixture is uniform.

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Abstract

The invention discloses a preserving stationary liquid for pathological tissues, and belongs to the technical field of sample processing. The preserving stationary liquid for pathological tissues is prepared from 12 to 15g of menthol, 10 to 20g of glycerin, 5 to 10ml of ethoxylation acetylenediol, 10 to 20g of chlorogenic acid, 30 to 70g of polyvinylpyrrolidone, 30 to 40g of sodium chloride, and 1000ml of pure water. The preserving stationary liquid for pathological tissues has the advantages that the formaldehyde solution is completely replaced; the property is stable, any toxic or side effect is avoided, the softness and hardness are proper after the pathological sample is subject to stationary treatment, the obvious shrinkage and hardening are avoided, the toughness is good, and the obvious deformation and discoloring are avoided; in the stationary process, the stationary liquid has no turbid or settling phenomenon.

Description

technical field [0001] The invention belongs to the technical field of specimen processing, and in particular relates to a pathological tissue preservation fixative. Background technique [0002] Biopsy pathological diagnosis is the first diagnosis in surgery and is the gold standard. Doctors can make a correct diagnosis based on the test results of the specimens and formulate the next treatment plan for the disease. The accuracy of pathology reports plays a decisive role in the diagnosis and treatment of diseases to a considerable extent. Correct handling of pathological specimens can not only provide an effective guarantee for pathological diagnosis and clinical diagnosis, but also maintain the patient's body and mind, and prevent disputes caused by improper specimen handling. Existing surveys have shown that the current specimen handling methods in the operating room cannot effectively prevent nursing errors. [0003] The most critical steps in the processing of pathol...

Claims

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Application Information

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IPC IPC(8): A01N1/02
CPCA01N1/0215
Inventor 魏严张婷王伟高霏姜旭
Owner NANYANG MEDICAL COLLEGE
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