The invention discloses a preparation method of giant spiny frog feed. Premna herbs, pumpkins, chicory and soybeans are taken for use, sesame-seed cake dregs, sweet potato starch, blood meal, fish meal and the like are added as ingredients, and then the process including dosing, thickening, extrusion forming, drying and the like is performed, so that the nutrient health care daily feed taking the shape of an earthworm and used for giant spiny frogs can be prepared finally. In consideration of feeding habits of the giant spiny frogs, the finished feed comprises the tasty blood and fish meal with fishy smell as well as the sesame-seed cake dregs; the sweet potato starch, the premna herb leaves, the pumpkin pectin and the mucilage of soybean milk protein are contained in the feed, so that the surface of the matured feed is smooth, the density of the finished product is enhanced, the toughness is good, and the feed is still cohered when falling into water; the ingredients contain high content of nutrient health care components such as protein, amylopectin and various vitamins and microelements; as the daily feed for the giant spiny frogs, the feed is good in palatability, so that the appetite can be promoted, the daily gain is improved, and the disease resistance of the giant spiny frogs can be enhanced. The feed is low in cost, is adequate and balanced in various nutrient substances, is convenient to process, store and feed, and is high in input-output ratio.