Preparation method of microbial pre-fermentation coating multilayer microcapsule
A microbial fermentation and microcapsule technology, applied in the field of microbial technology and biology, can solve problems such as inappropriate microbial packaging, and achieve the effects of convenient reuse, reduced production cost, and good solubility
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[0054] Example 1
[0055] The yeast double-layer microcapsules described in this example are prepared through the following steps:
[0056] (1) Preparation of single-layer microcapsules: Under aseptic conditions, take the aseptically treated sodium alginate solution with a concentration of 1 g / L, add the yeast seed solution in the logarithmic growth phase to be coated, and mix evenly. to 10 6 The density is about cfu / ml; and the mixed solution obtained above is filtered with a sterile filter with a diameter of less than 0.22 μm, and the flow rate is 4 m. 3 / h high-pressure air extrusion is sprayed into the aseptically treated calcium chloride solution with a concentration of 0.1mol / L through a hollow circular drop-shaped nozzle, stirred at a slow speed and solidified for 20min, and the supernatant is filtered out to obtain the required The single-layer microcapsules are washed with sterile water;
[0057] (2) Preparation of double-layered microcapsules: The single-layered m...
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[0060] Example 2
[0061] The yeast double-layer microcapsules described in this example are prepared through the following steps:
[0062] (1) Preparation of single-layer microcapsules: Under aseptic conditions, take the sodium alginate solution with a concentration of 3 g / L after aseptic treatment, add the yeast seed solution to be coated, and mix evenly to 10 6 The density is about cfu / ml; and the mixed solution obtained above is filtered with a sterile filter with a diameter of less than 0.22 μm, and the flow rate is 6 m. 3 / h high-pressure air is sprayed into the aseptically treated calcium chloride solution with a concentration of 0.3mol / L through a hollow circular drop-shaped nozzle, stirred at a slow speed and solidified for 20min, and the supernatant is filtered out to obtain the required monohydrate. layer microcapsules and wash with sterile water;
[0063] (2) Preparation of double-layered microcapsules: adding the single-layered microcapsules obtained in step (1)...
Example Embodiment
[0066] Example 3
[0067] The yeast double-layer microcapsules described in this example are prepared through the following steps:
[0068] (1) Preparation of single-layer microcapsules: Under aseptic conditions, take the sodium alginate solution with a concentration of 1 g / L after aseptic treatment, add the yeast seed liquid to be coated, and mix evenly; The mixed solution is filtered with a sterile filter with a diameter of less than 0.22 μm, and the flow rate is 4 m. 3 / h high-pressure air is sprayed into the aseptically treated calcium chloride solution with a concentration of 0.1 mol / L through a hollow circular drop nozzle, stirred uniformly and solidified, and the supernatant is filtered out to obtain the desired single-layer microcapsules , and wash with sterile water;
[0069] (2) Preparation of double-layered microcapsules: The single-layered microcapsules obtained in step (1) were added to an acidic buffer solution of chitosan with an initial concentration of 3 g / L...
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