Preparation method of cell wax block on the basis of bloody cell specimen and the cell wax block

Abstract

The invention relates to a preparation method of a cell wax block on the basis of a bloody cell specimen and the cell wax block and relates to the technical field of biology. The preparation method includes the steps of: 1) washing the bloody cell specimen with a first immobilizing solution and performing initial centrifugation, collecting a first precipitate sample and adding a second immobilizing solution, uniformly mixing the liquid, and performing secondary centrifugation to prepare a second precipitate; 2) dehydrating the second precipitate, performing transparent treatment, soaking the second precipitate in wax and embedding the second precipitate. The first immobilizing solution is an ethanol-acetic acid solution prepared by mixing ethanol, volume fraction being 24-25.5%, and aceticacid according to the volume ratio of 18-19:1. The preparation method is high in preparation efficiency and has simple operation. The prepared cell wax block can be preserved permanently, contains large quantity of cell components, has complete cell structure, can form clear image under high-magnification background, and can effectively increase accuracy of diagnosis.

Description

technical field [0001] The invention relates to the field of biotechnology, and in particular to a method for preparing a blood cell sample-based cell wax block and the cell wax block. Background technique [0002] Exfoliative cytology examination is one of the effective methods for tumor diagnosis. The small amount of hydrothorax and ascites absorbed during the preparation of ordinary smears cannot represent the status of the entire hydrothorax and ascites. Examine most of the cells in the pleural and ascites in order to observe the morphology of most cells, and the cell wax block is easy to preserve, which greatly improves the sensitivity and specificity of cytopathological diagnosis, and makes up for the limited and insufficient production of conventional cell smears. The inadequacies of multiple stains warrant further research and prospective studies. [0003] However, the fixed time of the existing cell wax block is long, and the cell loss is obvious, which affects the...

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Problems solved by technology

[0003] However, the fixed time of the existing cell wax block is long, and the cell loss is obvious, which affects the diagnosis result. At the same time, due to the existence of a large number of red blood cells in the existing blood cell sample, the quality of the wax block and the diagnosis result are affected. For some difficult cases Immunohistochemical staining of cells will also affect the judgment of positive results