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A rat eyeball tissue cell fixative and its preparation method and application method

A technology of tissue cells and fixation methods, applied in the application, preparation of test samples, preservation of human or animal bodies, etc., can solve problems such as adverse effects on the environment and operators' health, and achieve easy identification and observation. The effect of good application value

Active Publication Date: 2021-11-23
江苏博赛孚医疗科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The use of aldehydes has adverse effects on the environment and the health of operators

Method used

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  • A rat eyeball tissue cell fixative and its preparation method and application method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Prepare tissue cell fixative:

[0042] 3.6 parts of NaH 2 PO 4 2H 2 O, 8.2 parts of Na 2 HPO 4 12H 2 O and 500 parts H 2 O mixed into liquid a;

[0043] Take 3 / 4 of the total amount of liquid a and mix it with 28 parts of formaldehyde to form liquid b;

[0044] Take a quarter of the total amount of liquid a and mix it with 2 parts of ethylene glycol, 1 part of sorbitol, 3 parts of isobutanol and 7 parts of higher carbon alcohol to form c liquid;

[0045] Mix liquid b and liquid c at 60° C. for 30 min to obtain the tissue cell fixative.

[0046] Fixing tissue cells: After the tissue cells are taken, add tissue cell fixative solution 10 times the volume of the tissue cells to the tissue cells, mix and fix, the mixing temperature is room temperature, and the mixing time is 3 hours.

Embodiment 2

[0048] Prepare tissue cell fixative:

[0049] 2.5 parts of NaH 2 PO 4 2H 2 O, 9.3 parts of Na 2 HPO 4 12H 2 O and 400 parts H 2 O mixed into liquid a;

[0050] Take 3 / 4 of the total amount of liquid a and mix it with 36 parts of formaldehyde to form liquid b;

[0051] Take a quarter of the total amount of liquid a and mix it with 1 part of ethylene glycol, 4 parts of sorbitol, 2 parts of isobutanol and 9 parts of higher carbon alcohol to form c liquid;

[0052] Mix liquid b and liquid c at 40° C. for 40 min to obtain the tissue cell fixative.

[0053] Fixing the tissue cells: After the tissue cells are taken, add tissue cell fixative solution 6 times the volume of the tissue cells to the tissue cells, mix and fix, the mixing temperature is room temperature, and the mixing time is 24 hours.

Embodiment 3

[0055] Prepare tissue cell fixative:

[0056] 3.05 parts of NaH 2 PO 4 2H 2 O, 8.75 parts of Na 2 HPO 4 12H 2 O and 450 parts H 2 O mixed into liquid a;

[0057] Take 3 / 4 of the total amount of liquid a and mix it with 32 parts of formaldehyde to form liquid b;

[0058] Take a quarter of the total amount of liquid a and mix it with 1.5 parts of ethylene glycol, 2.5 parts of sorbitol, 2.5 parts of isobutanol and 8 parts of higher alcohols to form c liquid;

[0059] Mix liquid b and liquid c at 45° C. for 35 min to obtain the tissue cell fixative.

[0060] Fixing the tissue cells: After the tissue cells are taken, add tissue cell fixative solution 8 times the volume of the tissue cells to the tissue cells, mix and fix, the mixing temperature is room temperature, and the fixation time is 13.5 hours.

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Abstract

The invention discloses a tissue cell fixative and its preparation method and application method. It comprises the following components in parts by weight: 28-36 parts of formaldehyde, 1-2 parts of ethylene glycol, 1-4 parts of sorbitol, isobutyl alcohol 2-3 parts of alcohol, 7-9 parts of high carbon alcohol, NaH 2 PO 4 2H 2 O 2.5~3.6 parts, Na 2 HPO 4 12H 2 O 8.2~9.3 parts, H 2 O 400-500 parts. The preparation method of tissue cell fixative comprises the following steps: adding 2.5 to 3.6 parts of NaH 2 PO 4 2H 2 O, 8.2 to 9.3 parts of Na 2 HPO 4 12H 2 O and 400-500 parts of H 2 O mixed into a liquid; get three-quarters of the total amount of the a liquid and 28 to 36 parts of formaldehyde and mix it into a b liquid; get a quarter of the total amount of the a liquid and 1 to 2 parts of ethylene glycol, 1-4 parts of sorbitol, 2-3 parts of isobutanol and 7-9 parts of higher carbon alcohol are mixed to form liquid c; liquid b and liquid c are evenly mixed to obtain the tissue cell fixative solution. The tissue cell fixative of the invention can well maintain the inherent shape of the tissue cells and is more environmentally friendly.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a rat eyeball tissue cell fixative and its preparation method and application method. Background technique [0002] In order to prevent the post-mortem changes of tissue cells, prevent the autolysis and corruption of tissue cells, and maintain the inherent shape of tissue cells. Usually a tissue cell fixative is required. [0003] Tissue cell fixative can prevent endogenous lysosomal enzymes from autolyzing their own tissues and cells, inhibit the growth of bacteria and molds, and keep tissue cells in their inherent shape. [0004] Traditional tissue cell fixatives generally use aldehydes. The use of aldehydes has adverse effects on the environment and the health of operators. [0005] Therefore, it is necessary to further improve the existing tissue cell fixative so that it can be more environmentally friendly on the basis of maintaining a good fixation effect. Contents of the ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N1/00G01N1/30
CPCA01N1/00G01N1/30
Inventor 王守立赵飞沈刚
Owner 江苏博赛孚医疗科技有限公司
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