Cell preservation liquid

A technology of preservation solution and cells, applied in the field of cell preservation solution, can solve the problems of aggravating the treatment cost and high price of patients, and achieve the effects of easy promotion, convenient operation and simple use

Active Publication Date: 2014-09-17
ZHENGZHOU KODIA BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the American Thinprep cytologic test cell preservation solution is a special reagent used in conjunction with its instrument (Thinpreper-2000), but it is expensive and increases the cost of treatment for patients

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Embodiment 1, the cell preservation solution preparation method of the present invention is,

[0034] Sodium chloride: 0.12g Disodium edetate: 0.42g;

[0035] PEG10000: 0.15mlProclin300: 0.01ml;

[0036] Methanol: 10.0 g Acetic acid-sodium acetate buffer: 88.0 ml.

[0037] Let the above-mentioned components be evenly stirred and dissolved.

Embodiment 2

[0038] Embodiment 2, the preparation method of the cell preservation solution of the present invention is,

[0039] Sodium chloride: 0.14g Disodium edetate: 0.05g;

[0040] PEG10000: 0.20mlProclin300: 0.15ml;

[0041] Methanol: 30.0 g Acetic acid-sodium acetate buffer: 64.0 ml.

[0042] Let the above-mentioned components be evenly stirred and dissolved.

Embodiment 3

[0043] Embodiment 3, the preparation method of the cell preservation solution of the present invention is,

[0044] Sodium chloride: 0.14g Disodium edetate: 0.30g;

[0045] PEG10000: 0.18mlProclin300: 0.20ml;

[0046] Methanol: 40.0 g Acetic acid-sodium acetate buffer: 70.0 ml.

[0047] Let the above-mentioned components be evenly stirred and dissolved.

[0048] The cell preservation solution prepared by the above examples reduces the cost of use during use, truly and effectively protects the integrity and fixity of cells, avoids cell dissolution and swelling, is easy to promote, has strong compatibility, and saves the preparation of samples time.

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PUM

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Abstract

The invention provides a cell preservation liquid which is composed of sodium chloride, ethylene diamine tetraacetic acid disodium, PEG10000, Proclin300, methanol, and an acetic acid-sodium acetate buffer solution. The cell preservation liquid reduces the use cost, really effectively protects the integrity and stationarity of cells, prevents cell dissolution and expansion, is easy to popularize and strong in compatibility, and saves the sample preparation time.

Description

technical field [0001] The invention relates to the technical field of biological preparations; in particular, it relates to a cell preservation solution. Background technique [0002] At present, normal human living cells have physiological functions, biological activities and complete cell morphology before shedding, which is of great value in biological research and medical testing. If it leaves the human body, the physiological function, biological activity and cell morphology of the exfoliated cells will also change. Therefore, how to effectively preserve the shape and structure of exfoliated cells is a problem that needs to be considered in cytological detection. Mainly from the traditional and routine, there are the following inspection methods: [0003] In the smear made by the traditional cytological examination method Pap smear (cervical smear), there are a large number of overlapping cells, and there are some miscellaneous cells that have no test value, which br...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/00
Inventor 张鹭鹭李小磊李先坤程昀静郭利杰
Owner ZHENGZHOU KODIA BIOTECHNOLOGY CO LTD
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