62 results about "Reticulocyte" patented technology

The invention discloses a reticulocyte recognition and grading system based on a blood smear, and particularly relates to the field of medical cell morphology examination, and the system comprises an image acquisition module, a staining normalization module, an instance segmentation module, an erythrocyte classification module, a reticulocyte grading module and a statistics output module. The image acquisition module adopts an automatic microscope platform to continuously scan the blood smear under a 100-time visual field, acquires a high-quality bright field channel image through an automatic focusing technology, and monitors image definition, contrast and illumination uniformity; the statistical output module is used for calculating the proportion and bluish violet intensity statistics of reticulocytes of each level, and generating a visual quality control result and a diagnostic report; according to the invention, full-automatic processing from samples to reports is realized, interpretability and high classification precision are both considered, subjective difference and omission ratio of manual microscopic examination are significantly reduced, and efficiency and consistency of clinical detection are improved.

Glycated Hb was measured in reticulocytes (RNA-positive cells) and was correlated to a decrease in RNA fluorescence intensity in a Log-linear dependent manner, with no statistically significant differences in slope or in the ordinate of the origin between individuals. The loss of RNA fluorescence intensity in reticulocytes observed by flow cytometry is directly related to the time they spend in circulation and consequently to their lifespan. By setting the time that reticulocytes spend to mature in the blood circulation and because the relation between the decrease in log RNA fluorescence intensity and the increase in log HbA1c fluorescence intensity is linear, the inventors are able to calculate the increase in HbA1c fluorescence intensity depending on the time and thus to determine the mean half-life of a RBC population. This new tool is noninvasive and requires only one single time point measurement. The present invention relates to a method for in vitro determination of the age of red blood cells of a set of red blood cells by using intracellular glycated hemoglobin measurement by flow cytometry and by using a standard curve generated from glycated hemoglobin measurement in reticulocyes.

The purpose of the present invention is to provide a means for making it possible to sort and count reticulocytes and platelets. The blood cells in a test specimen are stained with a fluorescent dye, fluorescence information, first scattered light information, and second scattered light information that are generated by irradiating the stained blood cells with light having a wavelength of from 315 nm to 600 nm and light having a wavelength of from 610 nm to 750 nm are acquired, and the fluorescence information, the first scattered light information, and the second scattered light information are acquired on the basis of the first scattered light information and the second scattered light information, and / or on the basis of the first scattered light information and the second scattered light information, and / or on the basis of the first scattered light information and the second scattered light information. The problem is solved by specifying blood cells other than leukocytes on the basis of the first scattered light information and the fluorescence information, and classifying reticulocytes and platelets from groups other than leukocytes on the basis of the fluorescence information and the second scattered light information.

Disclosed is a method for analyzing blood cells, the method including: acquiring first scattered light information, second scattered light information, and fluorescence information generated by irradiating a measurement sample that contains a particle stained with a fluorescent dye with a light of a first wavelength and a light of a second wavelength; identifying blood cells other than white blood cell from the particles in the measurement sample, with reference to the first scattered light information and the second scattered light information, and / or, with reference to the first scattered light information and the fluorescence information; and classifying the blood cells other than white blood cell into reticulocyte and platelet, with reference to the fluorescence information and the second scattered light information, the first wavelength being 315 nm or longer and 600 nm or shorter, and the second wavelength being 610 nm or longer and 750 nm or shorter.

The invention discloses an image processing method and device for judging suspension red blood cell hemolysis. The method comprises the following steps: acquiring a blood image set; the blood image set comprises a plurality of blood images and corresponding label information; acquiring a blood parameter information set; the blood parameter information set comprises a plurality of blood parameters and corresponding label information; the blood parameters comprise hemoglobin concentration, reticulocyte concentration, indirect bilirubin concentration, lactic dehydrogenase concentration and globin concentration; utilizing the blood image set and the blood parameter information set to construct a blood hemolysis composite detection model; based on the blood hemolysis composite detection model, processing the collected blood image and blood parameters to obtain hemolysis detection result information; the hemolysis detection result information is used for representing erythrocyte hemolysis information of the blood corresponding to the collected blood image and blood parameters.

A method for staining reticulocytes includes staining reagent A and stabilizing reagent B; stabilizing reagent B is used M seconds after staining reagent A is mixed with the sample; M is greater than or equal to 20 seconds; staining reagent A includes staining agent A10, adjusting salt C10, and water; staining agent A10 is neomethylene blue; the concentration of neomethylene blue in staining reagent A is 0.8 × 10⁻⁶. ‑4 g / ml ~ 8.0 × 10 ‑4 g / mL; adjusting salt C10 is used to provide a low osmotic pressure environment and a pH range of 7.0–8.0; stabilizing reagent B includes staining stabilizer B10, adjusting salt D10, and water; the staining stabilizer B10 in stabilizing reagent B includes aldehydes. A unique two-step reagent formulation and method are used to achieve rapid staining of reticulocytes in a step-by-step manner, achieving successful staining in just 20 seconds, and the staining effect can be maintained for a sufficiently long time, exceeding 120 minutes.

Provided are a method for preparing reticulocyte simulating particles and platelet simulating particles, and a reference control. The method for preparing the reticulocyte simulating particles comprises: staining mammalian anucleated red blood cells having a volume of 60-120 fL with a protein fluorescent dye activated by N-hydroxysuccinimide, and fixing the anucleated red blood cells to prepare the reticulocyte simulating particles. The platelet simulating particles are prepared from mammalian anucleated red blood cells having a volume of 2-25 fL, and the steps of preparing the platelet simulating particles are the same as that for the reticulocyte simulating particles. The preparation method comprises: using an protein fluorescent dye activated by N-hydroxysuccinimide to stain mammalian anucleated red blood cells having different volumes, so as to respectively obtain reticulocyte simulating particles and platelet simulating particles.

The present disclosure relates to synthetic Plasmodium reticulocyte binding protein 2b (mRBP2b) antigens that have improved properties such as thermal stability, as well as the use of these antigens to detect a Plasmodium infection in a diagnostic device or for use in a vaccine.

The invention belongs to the technical field of pharmaceutical preparations, and particularly relates to application of a qi-tonifying and blood-maintaining preparation in preparation of a medicine for treating thalassemia. Researches show that the preparation for tonifying qi and maintaining blood is used for treating thalassemia and can effectively improve the anemia state and iron metabolism disorder of patients. Specifically, the medicine can significantly improve the hemoglobin level, increase the red blood cell count and the red blood cell average volume, and improve the serum iron content, the ferritin content and the reticulocyte proportion. The results show that the qi-tonifying and blood-maintaining preparation has the effect of promoting the bone marrow hematopoiesis function and can regulate and optimize the iron metabolism process. The invention provides an economical, effective and convenient treatment scheme for the thalassemia patient.

This invention relates to erythroid cells, such as reticulocyte cells, engineered to express a variant protein which comprises one or more mutations which increase the level of the variant protein in the cytosol of the cells. The invention also relates to erythroid cells, such as reticulocyte cells, genetically engineered to overexpress antioxidant proteins and / or an intrinsically disordered proteins (IDPs) in their cytosol and variants of such proteins which facilitate their overexpression in the cytosol of cells. Modification of the cells in this way enhances their storage, whether by refrigeration, freezing, lyophilisation or another storage method. The cells of the invention can be used to deliver therapeutic proteins or polypeptides.

This invention relates to antibodies for the treatment or prevention of malaria.

Disclosed are a blood analysis system, method, device, and computer storage medium. The blood analysis system includes: a sample collection device for collecting a blood sample and transferring the blood sample to a sample preparation device; a sample preparation device for processing the blood sample using a reagent to prepare a sample solution to be tested; an impedance detection device for detecting the sample solution to be tested to obtain an impedance pulse signal of cells in the sample solution to be tested; and a data processing device for performing the following steps: obtaining a blood cell histogram of the sample solution to be tested based on the impedance pulse signal, inputting the blood cell histogram into a pre-trained deep learning neural network model to obtain blood cell parameters output by the deep learning neural network model, wherein the blood cell parameters are at least one of the following: a reticulocyte parameter, a reticulocyte platelet parameter, and a red blood cell fragment parameter; and the deep learning neural network model is obtained by training blood training samples labeled with blood cell parameters using a machine learning algorithm.

The invention relates to a reticulocyte recognition AI training method and a calculation processing and storage device. The method comprises the following steps: dyeing blood to obtain a blood sample; cell nucleus substances of reticulocytes in the blood sample are dyed; flatly spreading the blood sample, and suspending red blood cells in the liquid to present a natural state; shooting the blood sample to obtain a picture; carrying out AI training by using the obtained pictures to obtain a reticulocyte AI feature data set A; according to the method, reticulocytes in a suspension state are dyed, so that the reticulocytes are different from erythrocytes, AI training is carried out by using a dyed picture, and an obtained AI feature data set A can effectively support AI detection for identifying the reticulocytes in the blood in the suspension state, and the blood cells are tiled and imaged, so that the identification accuracy of the reticulocytes in the blood in the suspension state is improved. And the AI identification and measurement of reticulocytes in a new-mode blood detection technology are effectively supported.

To provide means that enables classification and counting of reticulocytes and platelets.SOLUTION: The above challenge is cleared by following the steps of; staining blood cells in a sample with a fluorescent dye; acquiring fluorescence information, first scattered light information, and second scattered light information generated by irradiating the stained blood cells with light of a wavelength in a range of 315 nm to 600 nm, inclusive, and light of a wavelength in a range of 610 nm to 750 nm, inclusive; identifying blood cells other than white blood cells on the basis of the first scattered light information and the second scattered light information and / or on the basis of the first scattered light information and the fluorescence information; and classifying reticulocytes and platelets from populations other than white blood cells on the basis of the fluorescence information and the second scattered light information.SELECTED DRAWING: Figure 21B

The present invention relates to a method of maturity classifying reticulocytes from a whole blood sample, comprising: staining the sample with a supravital agglutinating dyeing reagent or a fluorescent agglutinating dye; illuminating the stained sample with a light beam to detect reticulocytes; determining for each reticulocyte the parameters of (i) a fraction (Λ) of the reticulum area (Ar) to the whole cell area (Ac); and (ii) a fraction (Γ) of the perimeter of the reticulum (Ur) to the reticulum area (Ar); and maturity classifying a reticulocyte into 1 of 4 major maturity classes according to the values determined for Λ and Γ.

The present invention relates to the use of light microscopy for reticulocyte maturity classification of stained reticulocytes. In particular, the present invention relates to a method for reticulocyte maturity classification from a whole blood sample, comprising: staining the sample with a supravital agglutination stain reagent or a fluorescent agglutination dye; illuminating the stained sample with a light beam to detect reticulocytes; determining for each reticulocyte the parameters (i) the fraction of reticular area (Ar) relative to the whole cell area (Ac) (Λ) and (ii) the fraction of reticular perimeter (Ur) relative to the reticular area (Ar) (Γ); and classifying the reticulocyte maturity into one of four main maturity classes according to the determined Λ and Γ values.

A method for analyzing blood cells includes acquiring first scattered light information, second scattered light information, and fluorescence information generated by irradiating a measurement sample that contains a particle stained with a fluorescent dye with a light of a first wavelength and a light of a second wavelength. The method includes identifying blood cells other than white blood cell from the particles in the measurement sample, with reference to the first scattered light information and the second scattered light information, and / or with reference to the first scattered light information and the fluorescence information. The method includes classifying the blood cells other than white blood cell into reticulocyte and platelet, with reference to the fluorescence information and the second scattered light information The first wavelength can be 315 nm or longer and 600 nm or shorter, and the second wavelength can be 610 nm or longer and 750 nm or shorter.

Provided are a method for preparing reticulocyte simulating particles and platelet simulating particles, and a reference control. The method for preparing the reticulocyte simulating particles comprises: staining mammalian anucleated red blood cells having a volume of 60-120 fL with a protein fluorescent dye activated by N-hydroxysuccinimide, and fixing the anucleated red blood cells to prepare the reticulocyte simulating particles. The platelet simulating particles are prepared from mammalian anucleated red blood cells having a volume of 2-25 fL, and the steps of preparing the platelet simulating particles are the same as that for the reticulocyte simulating particles. The preparation method comprises: using an protein fluorescent dye activated by N-hydroxysuccinimide to stain mammalian anucleated red blood cells having different volumes, so as to respectively obtain reticulocyte simulating particles and platelet simulating particles. The fluorescence and volume direction thereof in the scatter diagram are similar to the scatter diagram distribution of reticulocytes, reticulated platelets and platelets in fresh blood. The prepared simulating particles have good stability and do not interfere with the counting and differentiation of other cell particles.

The application discloses a reticulocyte identification and grading system based on blood smears, and particularly relates to the field of medical cell morphology inspection, and comprises an image acquisition module, a staining normalization module, an instance segmentation module, an erythrocyte classification module, a reticulocyte grading module and a statistical output module; the image acquisition module: adopts an automatic microscope platform to continuously scan blood smears under a 100-fold field of view, collects high-quality bright field channel images through automatic focusing technology, and monitors image definition, contrast and illumination uniformity; the statistical output module: calculates the proportion of reticulocytes at all levels and blue-purple intensity statistics, and generates visual quality control results and diagnosis reports; the application realizes full-automatic processing from samples to reports, takes into account interpretability and high classification accuracy, significantly reduces subjective differences and missed detection rates of manual microscopy, and improves the efficiency and consistency of clinical detection.

The present invention provides the use of 18β-glycyrrhetinic acid for improving the hematopoietic capacity of renal anemia. The present invention discovers and verifies for the first time that 18β-glycyrrhetinic acid stimulates the production of endogenous EPO, thereby improving the body's hematopoietic capacity. Short-term supplementation of 18β-glycyrrhetinic acid can significantly increase the level of erythropoietin, thereby promoting the production of reticulocytes. Long-term supplementation of 18β-glycyrrhetinic acid can improve indicators such as red blood cell count and alleviate the symptoms of renal anemia. The present invention provides a natural, safe, and highly effective active ingredient that improves the body's hematopoietic capacity, provides a scientific basis for basic research on drugs for the clinical treatment and prevention of renal anemia, and has significant application value.

The invention relates to a blood cell analyzer and a blood cell analysis method. The method comprises the following steps: mixing a part of a sucked blood sample to be detected, a diluent and a fluorescent staining agent in a first reaction tank to prepare a first determination sample, and obtaining first optical information generated after particles in the first determination sample are irradiated by light; mixing the other part of the blood sample to be detected with a hemolytic agent in a second reaction tank to prepare a second determination sample, and obtaining colorimetric optical information of the second determination sample; obtaining a reticulocyte detection result, a platelet detection result, an erythrocyte detection result and a first leukocyte detection result of the to-be-detected blood sample based on the first optical information, wherein the first leukocyte detection result at least comprises a lymphocyte percentage, a mononuclear cell percentage and a granulocyte percentage; and determining the hemoglobin concentration of the to-be-detected blood sample based on the colorimetric method optical information. According to the application, the reagent cost required for obtaining the detection result of the to-be-detected blood sample can be reduced.

1. The name of the product of this design: Fluorescence Blood Cell Analyzer (CF900). 2. Purpose of the product of this design: for blood sample counting, five-category classification of white blood cells, measurement of hemoglobin concentration, measurement of reticulocytes, measurement of nucleated red blood cells, measurement of body fluid cells and measurement of erythrocyte sedimentation rate in clinical examination. 3. The design key point of this appearance design product lies in the shape. 4. The picture or photo that best illustrates the design points: Stereoscopic diagram 1.

This invention relates to erythroid cells, such as reticulocyte cells, engineered to express a variant protein which comprises one or more mutations which increase the level of the variant protein in the cytosol of the cells. The invention also relates to erythroid cells, such as reticulocyte cells, genetically engineered to overexpress antioxidant proteins and / or an intrinsically disordered proteins (IDPs) in their cytosol and variants of such proteins which facilitate their overexpression in the cytosol of cells. Modification of the cells in this way enhances their storage, whether by refrigeration, freezing, lyophilisation or another storage method. The cells of the invention can be used to deliver therapeutic proteins or polypeptides.

The invention discloses a fluorescent dye composition and application thereof in biological sample dyeing, a reticulocyte detection reagent comprises a dye reagent and a diluent, and an anionic surfactant and an anionic compound contained in a buffer solution are utilized to reduce the thickness of a cell membrane and accelerate the rate that a fluorescent dye permeates into cells, so that the detection sensitivity of the reticulocyte is improved. Meanwhile, non-specific binding of the dye and red blood cells can be inhibited, several kinds of non-nucleic acid specific dyes are added, and a large number of binding competitive relations existing in a reaction system are adjusted by controlling the proportion of the added main dye to the added auxiliary dye and increasing reaction kinetics between nucleic acid and the dyes; the possibility of combining non-specific binding sites of a blood mixture through competition of secondary staining and primary staining is achieved, the fluorescence background is reduced, RBC fragments and low-fluorescence PLT are effectively distinguished, and the counting accuracy of the PLT is improved; high-fluorescence RET and leukocytes can be effectively distinguished, and RET classification counting accuracy is improved.

The invention specifically discloses a method and a system for predicting thrombocytopenic purpura, electronic equipment and a storage medium, and relates to the technical field of biological medicines. The invention provides a new prediction model (including platelet count, reticulocyte percentage, indirect bilirubin, broken red blood cells and a ratio of lactic dehydrogenase to normal high value) to help clinicians to early identify TTP patients, improve the TTP diagnostic rate, reduce the misdiagnosis rate, and improve the accuracy of TTP diagnosis on the basis of improving the diagnostic rate. The method has important guiding significance in the aspects of early treatment of patients, improvement of prognosis and the like.

The invention provides a preparation method of polyethylene glycol amphotericin B micelles, and belongs to the field of biological medicine manufacturing. The method comprises the following steps: reacting methoxy polyethylene glycol with lactide at 110-130 DEG C for 6-12 hours to obtain a block copolymer; the segmented copolymer and 2-(diisopropylamino) ethylamine are subjected to a reaction for 12-24 h, and a terpolymer is obtained; the terpolymer and 4-(hydroxymethyl) phenylboronic acid pinacol ester are subjected to a reaction for 12-36 h, and a quadripolymer is obtained; co-loading the quadripolymer and amphotericin B to obtain a micelle core; extracting a denucleated reticulated erythrocyte membrane from whole blood; and wrapping the outer surface of the micelle core with the denucleated reticulated erythrocyte membrane to obtain the polyethylene glycol amphotericin B micelle. Through the step-by-step design of intelligent response drug loading and bionic delivery camouflage, the dual purposes of'curative effect improvement and toxicity reduction 'of amphotericin B are achieved from three dimensions of accurate drug release, effective focus enrichment and normal tissue protection.

To provide means of performing blood cell analysis using a reagent that can be commonly used for classification of white blood cells and classification of reticulocytes and platelets, and can be used for blood cell analysis using multiple types of light.SOLUTION: The above challenge is cleared by staining blood cells in a sample using a cyanine-based fluorescent dye that can be excited by light of a wavelength in a range of 315 nm to 600 nm, inclusive, and can bind to nucleic acids.SELECTED DRAWING: Figure 18

A method, just requiring simple pretreatment, of concentration measurement for reticulocytes or nucleated erythrocytes includes a staining step, a removal step, and a measurement step. In the staining step, reticulocytes or nucleated erythrocytes among red blood cells contained in a blood specimen are stained. In the removal step, the blood specimen is mixed with a surfactant to extract hemoglobin from the red blood cells. In the measurement step, using the blood specimen having undergone the staining step and the removal step, the concentration of the reticulocytes or nucleated erythrocytes contained in the blood specimen is measured.

A method for dilution of a blood sample for analysis and to an apparatus for implementation of this method is provided.In the method, an aliquoting device is used, making it possible to carry out a single collection, to form a first dilution in a chamber, to collect a portion of the first dilution in order to form a second dilution in another chamber, to count the blood cells in the first and the second chamber, to carry out a differentiation based on the first dilution, to rinse the first chamber, to form a third dilution based on a quantity of first-dilution liquid remaining in the aliquoting device, then to carry out a differentiation of reticulocytes based on this third dilution.

The present application relates to a blood cell analyzer and a blood cell analysis method. One part of a suctioned blood sample to be detected, a diluent and a fluorescent coloring agent are mixed in a first reaction tank to prepare a first measurement sample, and first optical information generated by particles in the first measurement sample when said particles are irradiated by light is obtained; the other part of said blood sample and a hemolytic agent are mixed in a second reaction tank to prepare a second measurement sample, and colorimetric method optical information of the second measurement sample is obtained; a reticulocyte detection result, a platelet detection result, an erythrocyte detection result and a first leukocyte detection result of said blood sample are obtained on the basis of the first optical information, the first leukocyte detection result at least comprising a lymphocyte percentage, a monocyte percentage and a granulocyte percentage; and the hemoglobin concentration of said blood sample is determined on the basis of the colorimetric method optical information. The present application allows for reduction of the reagent costs required for obtaining the detection result of a blood sample to be detected.