3 results about "Erythroblast" patented technology

The present disclosure provides techniques for detecting blood disorders using cell-free DNA in a blood sample, e.g., using plasma or serum. For example, an analysis can target one or more differentially methylated regions specific to a particular blood cell lineage (e.g., erythroblasts). Methylation levels can be quantified from the analysis to determine the amount of methylated or unmethylated DNA fragments in the cell-free mixture of the blood sample. The methylation levels can be compared to one or more cutoff values, e.g., corresponding to a normal range for the particular blood cell lineage, as part of determining a level of a blood disorder.

A method, just requiring simple pretreatment, of concentration measurement for reticulocytes or nucleated erythrocytes includes a staining step, a removal step, and a measurement step. In the staining step, reticulocytes or nucleated erythrocytes among red blood cells contained in a blood specimen are stained. In the removal step, the blood specimen is mixed with a surfactant to extract hemoglobin from the red blood cells. In the measurement step, using the blood specimen having undergone the staining step and the removal step, the concentration of the reticulocytes or nucleated erythrocytes contained in the blood specimen is measured.

The present disclosure, in various aspects and embodiments, provides methods for generating hematopoietic lineages for cell therapy, including erythroid progenitor cells, progenitor erythroblasts, granulocyte-macrophage progenitor cells (GMPs), and megakaryocyte erythroid progenitor cells (MEPs), and erythroid cells. In various embodiments, the invention provides for efficient ex vivo processes for developing such hematopoietic lineages, including but not limited to progenitor erythroblast cells and erythroblast cell lineages, from human induced pluripotent stem cells (iPSCs). Cells generated according to the disclosure in various embodiments are functional and / or more closely resemble the corresponding lineage isolated from peripheral blood or bone marrow. The present invention also provides isolated cells and cell compositions produced by the methods disclosed herein, as well as methods for cell therapy.