A method for separating and identifying nucleated red blood cells in blood

A nuclear red blood cell and blood technology, applied in the field of blood separation, identification and detection, can solve the problems of high cost, cumbersome operation and high technical requirements
CN101122601BInactive Publication Date: 2011-11-30孙艳萍
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
孙艳萍
Publication Date
2011-11-30
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a method for separating and identifying nucleated erythrocytes in blood, which comprises the following steps: (1) preparing reagents; (2) preparing anti-degumming glass slides; Operation method to obtain nucleated red blood cells in the blood. The method for separating and identifying nucleated red blood cells in blood of the present invention can effectively identify nucleated red blood cells, all 40 samples can detect nucleated red blood cells, the detection rate is 100%, and 2×106 to 6×106 nucleated red blood cells were found in each case ml. The present invention is easy to identify nucleated red blood cells after benzidine staining, and can quickly obtain single nucleated red blood cells by skilled micromanipulation, laying a good preliminary foundation for applying single nucleated red blood cells to gene analysis.
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Description

technical field

[0001] The invention relates to the technical field of blood separation, identification and detection, in particular to a method for separating and identifying nucleated red blood cells in blood. Background technique

[0002] Separation of blood samples into various cellular components requires adjustment of blood sample handling / processing techniques accordingly. A conventional method of separating a whole blood sample into its various cellular components is by centrifugation. While effective, this method is labor intensive, relatively inefficient and requires manual control of the cellular fraction of the blood sample.

[0003] The separation of nucleated red blood cells is the beginning of genetic analysis, and it is also one of the more difficult steps. Scholars from various countries have tried various methods to separate nucleated red blood cells, but have not found a direct, reliable and efficient way to separate them.

[0004] Attempts to carry out...

Claims

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