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Method for inducing direct transdifferentiation of umbilical cord mesenchymal stem cells into erythroblasts

A qualitative stem cell and transdifferentiation technology, applied in the field of stem cells, can solve the problems of limited quantity, difficulties in erythroblasts, low efficiency of directed differentiation, etc., and achieve the effect of sufficient sources and easy access

Active Publication Date: 2016-12-07
黄兵 +1
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Problems solved by technology

This method solves the difficulties in the production of erythroblasts capable of synthesizing adult hemoglobin and denucleation (such as embryonic stem cells and induced pluripotent stem cells), quantity limitations (clinical-grade mature erythrocytes cannot be obtained), and directed differentiation in the existing artificial hematopoietic technology. (Differentiate into a higher proportion of other types of blood cells) Low efficiency technical bottle diameter problem

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  • Method for inducing direct transdifferentiation of umbilical cord mesenchymal stem cells into erythroblasts
  • Method for inducing direct transdifferentiation of umbilical cord mesenchymal stem cells into erythroblasts
  • Method for inducing direct transdifferentiation of umbilical cord mesenchymal stem cells into erythroblasts

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Embodiment Construction

[0039] Attached below picture The specific embodiment of the present invention is described in detail:

[0040] Such as figure 1 As shown, a method for inducing mesenchymal stem cells to transdifferentiate into erythroblasts, comprising the following steps:

[0041] S1, isolating and purifying homogeneous umbilical cord mesenchymal stem cells;

[0042] S2, selecting small RNA molecules that can be used for stem cell epigenetic regulation;

[0043] S3, preparation of small nucleic acid polypeptide nanoparticles and cell transfection;

[0044] S4, expansion and culture of erythroblasts after transfection.

[0045] Wherein, a chemotactic fluid is added in step S1, and the chemotactic fluid refers to the chemotactic SDF-SDF- 1 / CXCL12, HGF, EGF, bFGF, PDGF-BB and IGF-1 six chemokines.

[0046] The small RNA molecules described in step S2 are multiple, and the multiple small RNA molecules include sequences of ANTI-181b, ANTI-155, ANTI-10b, ANTI-24, ANTI-221 and siR-EID3 molec...

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Abstract

The invention discloses a method for inducing direct transdifferentiation of mesenchymal stem cells into erythroblasts, and discloses a method for rapidly inducing the direct transdifferentiation of the mesenchymal stem cells (MSCs) into the erythroblasts (EBCs). The method comprises the following steps: S1, separating and purifying the homogeneous umbilical cord mesenchymal stem cells; S2, selecting small RNA molecules applicable to the epigenetic regulation of the stem cells; S3, preparing small nucleic acid polypeptide nanoparticles and conducting cell transfection; and S4, conducting amplification culture on the transfected erythroblasts. According to the method disclosed by the invention, neonatal umbilical cord mesenchymal stem cells, which are easily acquired, sufficient in source, free from ethical and moral problems and safe and effective, are taken as an induced object and six small RNAs molecules are transfected, so that the large-scale and rapid direct transdifferentiation of the stem cells into the erythroblasts under the action of a special serum-free medium is achieved; and the technical difficulties in the prior art of the artificial hematopoietic technology on the limitation of the source and the quantity as well as directional differentiation of the erythroblasts are solved.

Description

technical field [0001] The invention relates to the technical field of stem cells, in particular to a method for inducing umbilical cord mesenchymal stem cells to directly transdifferentiate into erythroblasts. Background technique [0002] Umbilical cord mesenchymal stem cells (UC-MSCs) have high differentiation potential and can differentiate in multiple directions. It has broad clinical application prospects in tissue engineering of bone, cartilage, muscle, tendon, ligament, nerve, liver, endothelium and cardiac muscle. The results of flow cytometry showed that the adherent UC-MSCs all expressed CD166, CD44, CD29, CD105, CD90, CD73, low expression of CD106, no expression of hematopoietic cell phenotype CD38, CD34, CD45 and endothelial cell phenotype CD31, Nor does it express HLA-DR: It has been reported that UC-MSCs were isolated from human umbilical cord, and the cell content and proliferation ability were superior to those of bone marrow MSCs and adipose MSCs, and thei...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/078C12N5/0775
Inventor 黄兵殷勤伟
Owner 黄兵
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