Method for transdifferentiation of somatic cells into mammary epithelial cells by using small-molecule compound through in-vitro induction

A technology of mammary gland epithelial cells and small molecule compounds, which is applied in the field of cell transdifferentiation, can solve the problems of lack of lactation function and limited proliferation ability of mammary gland epithelial cells, and achieve the effect of avoiding the lack of lactation function and the limited proliferation ability in vitro
CN110951673AActive Publication Date: 2020-04-03GUANGXI UNIV +1
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
GUANGXI UNIV
Publication Date
2020-04-03

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Abstract

The invention provides a method for transdifferentiation of somatic cells into mammary epithelial cells by using a small-molecule compound through in-vitro induction. Expression of TGFbeta R1 and related loci thereof is inhibited, and transdifferentiation of somatic cells into mammary epithelial cells through in-vitro induction is achieved. By adopting the method, the blank of a technique for transdifferentiation of fibroblast into mammary epithelial cells through induction of the small-molecule compound is made up, and a research platform for research of in-vitro research on mammary gland bioreactors, mammary development and differentiation, breast cancer and ransdifferentiation of fibroblast into other types of functional cells is provided.
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Description

technical field

[0001] The invention belongs to the technical field of cell transdifferentiation, and in particular relates to a method for inducing somatic cell transdifferentiation into mammary gland epithelial cells in vitro by using a small molecule compound. Background technique

[0002] Mammary gland epithelial cells are an in vitro model for studying mammary gland growth and development, lactation mechanism, and verifying the effectiveness of mammary gland tissue-specific expression vectors. At present, most primary mammary epithelial cells are cultured by collagenase digestion and tissue block culture. The purer epithelial cells can be obtained by digesting mammary gland tissue with collagenase and then undergoing density gradient centrifugation. The tissue block culture method is simple in operation, saves tissue samples, and avoids the adverse effects of digestion and centrifugation on cells. However, it takes a long time for cells to grow out of the tissue block...

Claims

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