76 results about "Ion Channel Protein" patented technology

A method to prepare viruses lacking ion channel activity is provided.

Assays for screening potential drugs or agents that can interact and potentially bind to cation channel proteins, and potentially have uses in treating conditions related to the function of cation channel proteins is provided, along with prokaryotic cation channel proteins mutated to mimic eukaryotic cation channels, which can then be used in assays of the present invention.

The invention provides methods for identifying modulators of ion channels without the use of recombinant cell lines over-expressing the ion channel proteins or the use of detection labels.

The invention features an optically-controlled biological device that includes a biological component comprising a non-excitable cell expressing a light-gated ion channel protein and capable of forming gap junction channels with a target cell, and an optical stimulation unit.

The present invention provides delivery systems for and methods of delivering ion channel protein genetic material to cardiac cells in areas adjacent to where an electrode is to be positioned in a patient's heart to improve or correct the signal to noise ratio of cardiac signals, such as the P-wave. More specifically, there is provided a system and method for delivering sodium ion channel proteins or nucleic acid molecules encoding sodium ion channel proteins to a site in the heart adjacent to an electrode to increase the expression of the same, thereby enhancing the cardiac signal amplitude and enabling improved sensing of cardiac signals by an implanted pacemaker.

The present invention relates to a method for labeling a membrane-localized protein by introducing a biotin target sequence tag into at least one loop domain of a membrane-localized protein. The method of the invention is useful for labeling an ion channel protein such as CFTR or mutants thereof. A method for identifying an agent which corrects protein misfolding of a membrane-localized protein is also provided.

The invention provides methods for identifying modulators of ion channels without the use of recombinant cell lines over-expressing the ion channel proteins or the use of detection labels.

Focal and segmental glomerulosclerosis (FSGS) is a kidney disorder of unknown etiology and up to 20% of patients on dialysis have this diagnosis. A large family with hereditary FSGS carries a missense mutation in the TRPC6 gene on chromosome 11q, encoding the ion channel protein Transient Receptor Potential Cation Channel 6. The missense mutation is a P112Q substitution, which occurs in a highly conserved region of the protein, enhances TRPC6-mediated calcium signals in response to agonists such as angiotensin II, and alters the intracellular distribution of TRPC6 protein. Previous work has emphasized the importance of cytoskeletal and structural proteins in proteinuric kidney diseases. Our findings suggest a novel mechanism for glomerular disease pathogenesis.

An ion channel drug screening method includes the following steps of affusing cells with photosensitive ion channel protein into a chip; performing light treatment to the cells with photosensitive ion channel protein, which is affused into the chip; recording a reference transmembrane electric signal; administration; recording the transmembrane electric signal after administration; analyzing the reference transmembrane electric signal before administration and an administration transmembrane electric signal after administration and judging the affect of a drug to ion channels. Controlling the ion channels by light, the ion channel drug screening method can uniformly control the switches of the ion channels of the cell and facilitate accurately knowing the affect of the drug to the ion channels, thereby improving the success ratio of ion channel drug screening. In addition, the invention also provides an ion channel drug screening device.

Humanized recombinant and monoclonal antibodies specific for the ectodomain of the influenza virus M2 ion channel protein are disclosed. The antibodies of the invention have anti-viral activity and may be useful as anti-viral therapeutics and/or prophylactic/vaccine agents for inhibiting influenza virus replication and for treating individuals infected with influenza.

The invention discloses a BmCP274 promoter of bombyx mori cuticular protein. The promoter consists of nucleotide sequences shown in SEQ ID No.1, can drive foreign proteins to specifically express at a front silk gland of the bombyx mori and can be used as a specific promoter at the front silk gland of the silkworm. The invention further discloses a recombinant expression vector containing the promoter, which provides a powerful tool for a next-step study on regulating ion channel protein expression at the front silk gland of the silkworm to affect the behavior of the bombyx mori and improve the performance of the silk fiber.

The invention relates to a non-invasive near-infrared optically-controlled nano-material for repairing damaged nerves. The non-invasive near-infrared optically-controlled nano-material converts near-infrared light into visible light and/or ultraviolet light. The non-invasive near-infrared optically-controlled nano-material is an up-converting fluorescent nano-material. The invention discloses newuse of the up-converting fluorescent nano-material. In a repairing process of neurons, electrical stimulation does not need to be applied to the neurons, animals do not need to be surgically implantedwith invasive fibers, and near-infrared light with high tissue penetrability is used for stimulating the up-converting fluorescent nano-material in organisms; the converted up-converting fluorescencecan activate photosensitive ion channel protein on the neuronal cell membrane, stimulate the neuronal cells to produce membrane potential changes and enhance the stimulation of the neural circuits, thus repairing the damaged neurons.

The invention discloses a cycloalkane amine compound of a general formula I or II, or pharmaceutically acceptable salt thereof, or substituted group defined in the description; the cycloalkane amine compound of the general formula I or II, or pharmaceutically acceptable salt can be used for preparing medicine for preventing and treating flue; as the M2 ion channel protein inhibitor, the compound can stop copy of the flue virus, and treat influenza, specifically the influenza A.

The invention provides recombined corynebacterium glutamicum, can be used for effectively solving the technical problem that the conventional corynebacterium glutamicum is low in foreign protein expression quantity. The peptidoglycan endonucleases gene mepA, cytomembrane surface anion protein gene porB and endoproteinase gene clpC in the corynebacterium glutamicum are knocked out, meanwhile ACB transporter gene Ncg10909 are over expressed. Moreover the invention further provides a preparation method for the recombined corynebacterium glutamicum and application thereof.

According to the novel human sensory neuron induction culture system provided by the invention, the combination of the small-molecule inhibitor LY2157299 and the growth factor is added into the serum-free basal culture medium, so that compared with a serum-containing induction method, the efficiency of converting pluripotent stem cells into sensory neurons is greatly improved, and the induction efficiency of the pluripotent stem cells is improved. In addition, the expression of various ion channel proteins is obviously improved, so that various induced pluripotent stem cells with different sources are successfully induced into sensory neurons.

The present invention relates to synthetic membranes and provides a solid supported membrane comprising: a support; a chromium layer; a gold layer; an alkyl monolayer, a lipid monolayer; and an ion channel protein, wherein the alkyl monolayer and the lipid monolayer form a bilayer, and further wherein a functionally reconstituted ion channel is supported, and methods for forming them. The invention further provides methods and applications of the membranes in rapid screening for pharmacological agents and as bioelectric smart sensors.

The invention relates to a method for screening hERG potassium ion channel agonist and detecting toxicity. Particularly, the invention firstly relates to a fusion protein, which contains a fragment (which is used as the N end of the fusion protein, is from a position between 1st to 85th amino acid residues of the N end of the nemathelminth ERG family potassium ion channels UNC-103 protein and has the length of 75 to 85 amino acid residues), hERG or a fragment at least containing S1-S6 transmembrane domains and cyclic nucleotide binding domains, and a fragment (which is used as the C end of the fusion protein, is from a position between 590th to 829th of amino acid residues of the C end of the UNC-103 protein and has the length of 220 to 240 amino acid residues). The invention also relates to a polynucleotide sequence coding the fusion protein, a relevant transgenic nemathelminth, a relevant screening method and application. The inventor builds an in-vivo hERG-intracellular-transport-influence-recognizable compound molecule screening method for screening hERG inhibitors or LQTS (long QT syndrome) relevant channel mutant functional correcting agents for the first time; the novel path and method are provided for hERG toxicity detection and LQTS treatment medicine screening.

The invention belongs to the technical field of tobacco gene engineering, and particularly relates to a tobacco chloride ion channel protein gene NtCLC-F and application thereof. The base sequence of the gene is as shown in SEQ ID NO.1. The tobacco chloride ion channel protein NtCLC-F is composed of 726 amino acid residues, wherein the 181st-500th amino acids are conserved chloride ion channel protein structural domains. The protein is related to the chloride ion content of plant leaves, and after the expression of the protein is reduced, the chloride ion content of the leaves is obviously increased. Preliminary research on a specific tobacco chloride ion channel NtCLC-F shows that the specific tobacco chloride ion channel NtCLC-F is highly related to the content of the tobacco chloride ions, and after the gene is silenced, the content of the tobacco chloride ions is obviously increased. Based on the characteristic, a certain application basis and reference can be provided for cultivation of a new tobacco variety with the chlorine ion content regulated and controlled.

The invention discloses a method for increasing the intracellular heme content of escherichia coli, and belongs to the field of metabolic engineering. The method comprises the following steps: knocking out a gene mscS for encoding a small-conductivity mechanical sensitive ion channel protein, knocking out a gene aroG for encoding 3-deoxy-arabinoheptulose-7 phosphate synthase, or overexpressing a gene hemA for encoding glutamyl-tRNA reductase in escherichia coli. The constructed recombinant bacteria are cultured in an LB culture medium, the heme content can reach 47.6 [mu]mol.L<-1> and is remarkably improved compared with that of a control strain, and the recombinant bacteria have wide application value.

The invention discloses eggplant potassium ion channel protein SmAKT1 as well as an encoding gene and an application thereof. The protein SmAKT1 has the amino acid sequence shown in SEQ ID NO.2, and anucleotide sequence encoding the gene of the protein is shown in SEQ ID NO.1. The gene has expression in roots, stems, leaves, flowers, fruits and sepals, and the expression quantity in a root systemis notably higher than that in other tissue. Under low-potassium and salt stress treatment, the expression quantity of the gene in the leaves and the root system has the trend of first rising and then dropping. The gene function of the protein SmAKT1 is also identified, the gene SmAKT1 is imported in a target defect type yeast strain or an arabidopsis thaliana mutant, and the stress resistance ofthe gene to low-potassium stress and salt stress can be improved. A theoretical basis is provided for cultivation of the low-potassium stress and salt stress resistant eggplants with a gene engineering technology in the future, and the gene has great application value.